Cited by 152Bone Metabolism and DiseasesBone health and osteoporosis researchBone and Dental Protein Studies

Effect of osteoclast activating factor from human leukocytes on bone metabolism.

Open access
Raisz, L G, Luben, R A, Mundy, G R, Dietrich, J W, Horton, J E, Trummel, C L·1975·Journal of Clinical Investigation

Abstract

The effects of osteoclast activating factor (OAF) released by normal human peripheral blood leukocytes cultured with phytohemagglutinin have been examined in organ culture. Like parathyroid hormone (PTH), OAF causes a rapid increased in the release of previously incorporated 45Ca from fetal rat bone after brief or continuous exposure; the bones also lose stable calcium and collagen content. The resorption response to OAF also resembles that of PTH in having a steep dose response curve and being only transiently inhibited by calcitonin and partially inhibited by increasing medium phosphate concentration. OAF-stimulated resorption was inhibited more effectively by cortisol than was PTH stimulation. The response to maximally effective doses of OAF was not enhanced by PTH or prostaglandin E2, but submaximal doses gave additive effects. Both OAF and PTH inhibit collagen synthesis in fetal rat calvaria at the concentrations that stimulate bone resorption.

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Crossref · 10.1172/jci108106

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  • Cytokines such as IL-1 and prostaglandins such as PGE2 can affect bone.

    “locally produced cytokines such as IL-1 and prostaglandins such as prostaglandin E 2 (PGE 2 ) can affect bone”

    From Pathogenesis of osteoporosis: concepts, conflicts, and prospects (2005) · cites this paper as [82]

    The Discussion reports that osteoclast-activating factor (OAF) secreted by human leukocytes stimulates bone resorption in fetal rat bone cultures, and that PGE2 can also affect resorption. It does not identify OAF as IL-1 or establish that PGE2 is locally produced. The claim's specific IL-1 and local-production details are therefore unsupported by this copy. (LLM, conf=0.84)

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Authors
Raisz, L. G., Luben, R. A., Mundy, G. R., Dietrich, J. W., Horton, J. E., & Trummel, C. L.
Affiliations
National Institutes of Health (US)
Venue
Journal of Clinical Investigation
Published
1975-08-01
Volume
56
Issue
2
Pages
408-413
ISSN
0021-9738 (ISSN-L)
OpenAlex
W2101357889
Cited by
152
References
14
Citations by year
2021: 1 · 2019: 2 · 2017: 1 · 2016: 1 · 2015: 2 · 2013: 1
FWCI
4.10
Type
journal article
Language
en
Open access
Locations
  • Journal of Clinical Investigation (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
  • Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
Keywords
osteoclast activating factor, bone resorption, bone metabolism, parathyroid hormone, calcitonin, cortisol, collagen synthesis, calcium release, leukocytes, phytohemagglutinin, prostaglandin E2, dose-response relationship, calvaria
MeSH terms
Animals, Bone and Bones, Bone Resorption, Calcitonin, Calcium, Calcium Radioisotopes, Collagen, Dose-Response Relationship, Drug, Female, Fetus, Humans, Indomethacin, Leukocytes, Organ Culture Techniques, Parathyroid Hormone, Phosphates, Pregnancy, Prostaglandins E, Time Factors, Rats

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