Effect of osteoclast activating factor from human leukocytes on bone metabolism.
Open accessAbstract
The effects of osteoclast activating factor (OAF) released by normal human peripheral blood leukocytes cultured with phytohemagglutinin have been examined in organ culture. Like parathyroid hormone (PTH), OAF causes a rapid increased in the release of previously incorporated 45Ca from fetal rat bone after brief or continuous exposure; the bones also lose stable calcium and collagen content. The resorption response to OAF also resembles that of PTH in having a steep dose response curve and being only transiently inhibited by calcitonin and partially inhibited by increasing medium phosphate concentration. OAF-stimulated resorption was inhibited more effectively by cortisol than was PTH stimulation. The response to maximally effective doses of OAF was not enhanced by PTH or prostaglandin E2, but submaximal doses gave additive effects. Both OAF and PTH inhibit collagen synthesis in fetal rat calvaria at the concentrations that stimulate bone resorption.
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Cytokines such as IL-1 and prostaglandins such as PGE2 can affect bone.
“locally produced cytokines such as IL-1 and prostaglandins such as prostaglandin E 2 (PGE 2 ) can affect bone”
From Pathogenesis of osteoporosis: concepts, conflicts, and prospects (2005) · cites this paper as [82]
The Discussion reports that osteoclast-activating factor (OAF) secreted by human leukocytes stimulates bone resorption in fetal rat bone cultures, and that PGE2 can also affect resorption. It does not identify OAF as IL-1 or establish that PGE2 is locally produced. The claim's specific IL-1 and local-production details are therefore unsupported by this copy. (LLM, conf=0.84)
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Molecular Theology, Immunophilosophy, and Autoimmune Disease
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Studies on the mechanisms by which 2-chloroadenosine stimulates bone resorption in tissue culture
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Kidney and Bone: Physiological and Pathophysiological Relationships
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Metabolic Bone Disease and Paget's Disease in the Elderly
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- Authors
- Raisz, L. G., Luben, R. A., Mundy, G. R., Dietrich, J. W., Horton, J. E., & Trummel, C. L.
- Affiliations
- National Institutes of Health (US)
- Venue
- Journal of Clinical Investigation
- Published
- 1975-08-01
- Volume
- 56
- Issue
- 2
- Pages
- 408-413
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC436600
- OpenAlex
- W2101357889
- Cited by
- 152
- References
- 14
- Citations by year
- 2021: 1 · 2019: 2 · 2017: 1 · 2016: 1 · 2015: 2 · 2013: 1
- FWCI
- 4.10
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
- Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
- Keywords
- osteoclast activating factor, bone resorption, bone metabolism, parathyroid hormone, calcitonin, cortisol, collagen synthesis, calcium release, leukocytes, phytohemagglutinin, prostaglandin E2, dose-response relationship, calvaria
- MeSH terms
- Animals, Bone and Bones, Bone Resorption, Calcitonin, Calcium, Calcium Radioisotopes, Collagen, Dose-Response Relationship, Drug, Female, Fetus, Humans, Indomethacin, Leukocytes, Organ Culture Techniques, Parathyroid Hormone, Phosphates, Pregnancy, Prostaglandins E, Time Factors, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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