The DOI matches its Crossref record; 56 of the 57 references it cites were found in a registry or an index; none of the 74 sources its claims cite is checked for support yet.
Effects of cigarette smoking and its cessation on lipid metabolism and energy expenditure in heavy smokers.
Open accessAbstract
The relationship between thermogenic and potentially atherogenic effects of cigarette smoking (CS) and its cessation was investigated. Heavy smokers (n = 7, serum cotinine > 200 ng/ml, > 20 cigarettes/d) were maintained on isoenergetic, constant diets for 2 wk, 1 wk with and 1 wk without CS. Stable isotope infusions with indirect calorimetry were performed on day 7 of each phase, after an overnight fast. CS after overnight abstention increased resting energy expenditure by 5% (not significant vs. non-CS phase; P = 0.18). CS increased the flux of FFA by 77%, flux of glycerol by 82%, and serum FFA concentrations by 73% (P < 0.02 for each), but did not significantly affect fat oxidation. Hepatic reesterification of FFA increased more than threefold (P < 0.03) and adipocyte recycling increased nonsignificantly (P = 0.10). CS-induced lipid substrate cycles represented only 15% (estimated 11 kcal/d) of observed changes in energy expenditure. De novo hepatic lipogenesis was low (< 1-2 g/d) and unaffected by either acute CS or its chronic cessation. Hepatic glucose production was not affected by CS, despite increased serum glycerol and FFA fluxes. Cessation of CS caused no rebound effects on basal metabolic fluxes. In conclusion, a metabolic mechanism for the atherogenic effects of CS on serum lipids (increased hepatic reesterification of FFA) has been documented. Increased entry of FFA accounts for CS-induced increases in serum FFA concentrations. The thermogenic effect of CS is small or absent in heavy smokers while the potentially atherogenic effect is maintained, and cessation of CS does not induce a rebound lipogenic milieu that specifically favors accrual of body fat in the absence of increased food intake.
Overview
CoChat Summary
TL;DR
In seven heavy smokers on a metabolic ward, acute cigarette smoking increased lipolysis and hepatic FFA reesterification but produced only a small, nonsignificant rise in resting energy expenditure. Smoking cessation did not induce a rebound lipogenic or reduced-fat-oxidation state that would favor body-fat gain in the absence of increased food intake.
Key findings
- Acute smoking after overnight abstinence increased resting energy expenditure by 4.6% (64.4±3.5 to 67.3±3.9 kcal/h), which was not statistically significant versus the non-smoking phase (P = 0.18).
- Acute smoking increased FFA flux by 77%, glycerol flux by 82%, and serum FFA concentrations by 73% (P < 0.02 for each), without significantly affecting fat oxidation.
- Hepatic reesterification of FFA increased more than threefold (0.59±0.48 to 2.03±0.54 µmol/kg per min; P < 0.03), while adipocyte recycling increased nonsignificantly (P = 0.10); lipid substrate cycles accounted for only 15% (estimated 11 kcal/d) of observed changes in energy expenditure.
- De novo hepatic lipogenesis was low (<1–2 g/d) and unaffected by acute smoking or cessation; hepatic glucose production was not affected, and cessation caused no rebound effects on basal metabolic fluxes.
Methods
- Seven heavy smokers (serum cotinine >200 ng/ml, >20 cigarettes/d) completed a 2-wk inpatient metabolic ward study with randomized 1-wk smoking and 1-wk non-smoking phases on a constant, weight-maintaining diet.
- On day 7 of each phase, after an overnight fast, subjects received stable isotope infusions ([1-13C]acetate, [d5]-glycerol, [1,2,3,4-13C]palmitate, and [6,6-d2]-glucose) with indirect calorimetry; they smoked one cigarette every 30 min from 9:00 a.m. to noon in the smoking phase or did not smoke.
- Rates of appearance were calculated by isotope dilution; FFA reesterification was calculated using the Wolfe/Klein method, and fractional de novo lipogenesis was measured by mass isotopomer distribution analysis; comparisons used two-factor repeated measures ANOVA.
Limitations
- The paper states that the 1-wk smoking and non-smoking periods were not long enough for differences in fasting serum lipoproteins to become apparent.
- The paper states that the absolute DNL method may underestimate DNL if tissues oxidize endogenous fat stores that did not traverse the circulation, and that the technique relies on several assumptions.
- Evident from the text: the sample was very small (n = 7) and restricted to heavy smokers, so findings may not generalize to light smokers; withdrawal scores were not measured.
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Integrity signals
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Statistics
Verification
Claims the paper makes
0 Valid · 0 Not valid · 39 Not checked · 103 Own results and interpretations
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Cessation of CS caused no rebound effects on basal metabolic fluxes.”
§ Effects of Cigarette Smoking and Its Cessation on Lipid Metabolism and Energy Expenditure in Heavy Smokers > Abstract
plainn = 7- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“REE was not significantly different after an overnight fast in the CS and non-CS phases (64.4±3.5 and 64.1±4.3 kcal/h, respectively; Fig. 2).”
§ Effects of Cigarette Smoking and Its Cessation on Lipid Metabolism and Energy Expenditure in Heavy Smokers > Results
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“CS-induced lipid sub- strate cycles represented only 15% (estimated 11 kcal/d) of observed changes in energy expenditure.”
§ Effects of Cigarette Smoking and Its Cessation on Lipid Metabolism and Energy Expenditure in Heavy Smokers > Abstract
hedgedstates a causen = 7- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“During the non-CS week, basal levels were undetectable (< 1.0 ng/ml).”
§ Effects of Cigarette Smoking and Its Cessation on Lipid Metabolism and Energy Expenditure in Heavy Smokers > Results
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: unclear — It is unclear whether the paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Hepatic reesterification of FFA increased more than threefold (P < 0.03)”
§ Effects of Cigarette Smoking and Its Cessation on Lipid Metabolism and Energy Expenditure in Heavy Smokers > Abstract
plainstates a causen = 7 ·- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
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- It may state two separate claims as one. 27
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Citations
Cites
Randle, P. J., Garland, P. B., Hales, C. N., & Newsholme, E. A. (1963) · The Lancet · doi:10.1016/s0140-6736(63)91500-9 · Cited by 5,093
Pharmacologic Aspects of Cigarette Smoking and Nicotine Addiction
Oates, J. A., Wood, A. J. J., & Benowitz, N. L. (1988) · New England Journal of Medicine · doi:10.1056/nejm198811173192005 · Cited by 969
Self-citationSmoking Cessation and Severity of Weight Gain in a National Cohort
Williamson, D. F., Madans, J., Anda, R. F., Kleinman, J. C., Giovino, G. A., & Byers, T. (1991) · New England Journal of Medicine · doi:10.1056/nejm199103143241106 · Cited by 623
Fredrickson, D. S., & Gordon, R. S. (1958) · Physiological Reviews · doi:10.1152/physrev.1958.38.4.585 · Cited by 568
Effect of Severe Burn Injury on Substrate Cycling by Glucose and Fatty Acids
Wolfe, R. R., Herndon, D. N., Jahoor, F., Miyoshi, H., & Wolfe, M. (1987) · New England Journal of Medicine · doi:10.1056/nejm198708133170702 · Cited by 467
Carlson, L. A., & Orö, L. (1962) · Acta Medica Scandinavica · doi:10.1111/j.0954-6820.1962.tb07203.x · Cited by 437
Increased 24-Hour Energy Expenditure in Cigarette Smokers
Hofstetter, A., Schütz, Y., Jéquier, E., & Wahren, J. (1986) · New England Journal of Medicine · doi:10.1056/nejm198601093140204 · Cited by 376
Measurement of de novo hepatic lipogenesis in humans using stable isotopes.
Hellerstein, M. K., Christiansen, M., Kaempfer, S., Kletke, C., Wu, K. K., Reid, J., Mulligan, K. M., Hellerstein, N. S., & Shackleton, C. H. L. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115206 · Cited by 361
Self-citationRandle, P. J. (1986) · Biochemical Society Transactions · doi:10.1042/bst0140799 · Cited by 311
THE METABOLIC EFFECTS OF EPINEPHRINE AND RELATED AMINES
Ellis, S. (1956) · Pharmacological Reviews · doi:10.1016/s0031-6997(25)06998-4 · Cited by 297
Research works printed but not linked: 2
- E. S. Katkin and S. B. Manuck, editors. Jai Press Inc., Greenwich, Cc. 97-129.
- Hellerstein, M. K., R. A. Neese, and J.-M. Schwarz. 1993. Measurement of absolute rates of hepatic de novo lipogenesis and re-esterification of free fatty acids in humans. Am. J. Physiol. In press.
Cited by
Pharmacology of Nicotine: Addiction and Therapeutics
Benowitz, N. L. (1996) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pa.36.040196.003121 · Cited by 589
Self-citationCigarette Smoking, Nicotine, and Body Weight
Audrain-McGovern, J., & Benowitz, N. L. (2011) · Clinical Pharmacology & Therapeutics · doi:10.1038/clpt.2011.105 · Cited by 571
Self-citationBenowitz, N. L., & Gourlay, S. G. (1997) · Journal of the American College of Cardiology · doi:10.1016/s0735-1097(97)00079-x · Cited by 529
Self-citationCigarette smoking and cardiovascular disease: pathophysiology and implications for treatment
Benowitz, N. L. (2003) · Progress in Cardiovascular Diseases · doi:10.1016/s0033-0620(03)00087-2 · Cited by 440
Self-citationStrawford, A., Antelo, F., Christiansen, M., & Hellerstein, M. K. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00093.2003 · Cited by 364
Self-citation
Metadata
- Authors
- Hellerstein, M. K., Benowitz, N. L., Neese, R. A., Schwartz, J. M., Hoh, R., Jacob, P., Hsieh, J., & Faix, D.
- Affiliations
- San Francisco General Hospital (US)
- Venue
- Journal of Clinical Investigation
- Published
- 1994-01-01
- Volume
- 93
- Issue
- 1
- Pages
- 265-272
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC293761
- OpenAlex
- W2113048216
- Semantic Scholar
- bf19d755f26608e0b1c7f1b52d3d661afabf17ff
- Cited by
- 155
- References
- 57
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 1 · 2023: 7 · 2022: 7 · 2021: 4 · 2020: 5 · 2019: 6 · 2018: 3 · 2017: 8 · 2016: 3 · 2015: 5 · 2014: 5 · 2013: 5 · 2012: 7
- FWCI
- 12.45
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by-nc-sa)
- Funders
- Deutsches Krebsforschungszentrum
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
- National Institutes of Health (RR-00083)
- National Institute on Drug Abuse (DA-2277)
- National Center for Research Resources (M01RR000083, RR 00083)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Smoking Behavior and Cessation
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
- Keywords
- cigarette smoking, smoking cessation, lipid metabolism, energy expenditure, fatty acid oxidation, hepatic lipogenesis, hepatic glucose production, serum lipids, atherogenic effects, thermogenesis, heavy smokers
- MeSH terms
- Calorimetry, Cotinine, Energy Metabolism, Fatty Acids, Nonesterified, Glucose, Glycerol, Humans, Liver, Models, Biological, Smoking, Biomarkers, Smoking Cessation, Adipocytes
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