The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
17 beta-Hydroxysteroid dehydrogenase type 2: chromosomal assignment and progestin regulation of gene expression in human endometrium.
Open accessAbstract
The cDNAs for two separate human 17 beta-hydroxysteroid dehydrogenases (17 beta-HSD) have been isolated and sequenced. The well-studied human placental cytosolic 17 beta-HSD (also referred to as estradiol dehydrogenase) preferentially catalyzes the reduction of estrone to estradiol-17 beta and the reduction of the C-20-ketone of progesterone to 20 alpha-dihydroprogesterone. This isoform of the enzyme has been referred to as 17 beta-HSD type 1 and localized to chromosome 17. A second 17 beta-HSD isoform (referred to as type 2) is localized in the endoplasmic reticulum of human trophoblast and is characterized by the preferential oxidation of the C-17 beta-hydroxyl group of C18- and C19-steroids and the C-20 alpha-hydroxyl group of 20 alpha-dihydroprogesterone. In this study, we determined the chromosomal localization of human 17 beta-HSD type 2, the expression of this gene in human endometrium, and the tissue distribution of the mRNA. We found that the human 17 beta-HSD type 2 gene is localized on chromosome 16, 16q24. 17 beta-HSD type 2 mRNA (approximately 1.5 kb) was identified in human endometrial tissues by Northern analysis of total RNA (10 micrograms). The highest levels of 17 beta-HSD type 2 mRNA were found in endometrial tissues obtained during the mid- to late secretory phase of the ovarian cycle (i.e., during the time of high plasma levels of progesterone). 17 beta-HSD type 2 mRNA levels were much greater in glandular epithelium than in the stromal cells isolated from secretory phase endometrium. The levels of 17 beta-HSD type 2 mRNA in secretory phase endometrium were approximately one-tenth that in villous trophoblast tissue from human placenta. We did not detect 17 beta-HSD type 1 mRNA in endometrial tissue by Northern analysis of total (10 micrograms) RNA. These findings are consistent with the view that the progestin-regulated 17 beta-HSD of the glandular epithelium of the human endometrium is primarily, if not exclusively, the product of the 17 beta-HSD type 2 gene. 17 beta-HSD type 2 mRNA was present in human placenta, liver, and small intestine; much smaller amounts, barely detectable by Northern analysis of poly(A)+ RNA, were present in prostate, kidney, pancreas, and colon, but not in heart, brain, skeletal muscle, spleen, thymus, ovary, or testis.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Progesterone via PGR induces retinoic acid production in endometrial stromal cells, which then acts on epithelial cells to induce HSD17B2 expression”
From Endometriosis and nuclear receptors (2019) · cites this paper as Casey et al., 1994
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“Instead, a cDNA encoding microsomal HSD17B2 was cloned (513) and found to be expressed in endometrium, placenta, and other tissues (514).”
From The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders (2011) · cites this paper as [514]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,460
High-Resolution Mapping of Human Chromosome 11 by in Situ Hybridization with Cosmid Clones
Lichter, P., Tang, C.-J. C., Call, K. M., Hermanson, G., Evans, G. A., Housman, D. E., & Ward, D. C. (1990) · Science · doi:10.1126/science.2294592 · Cited by 1,330
Placental Estrogen Biosynthesis During Human Pregnancy1
SIITERI, P. K., & MACDONALD, P. C. (1966) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-26-7-751 · Cited by 479
Self-citationWu, L., Einstein, M., Geissler, W. M., Chan, H. K., Elliston, K. O., & Andersson, S. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)31480-7 · Cited by 437
Self-citationOrigin of Estrogen in Normal Men and in Women with Testicular Feminization*
MacDonald, P. C., Madden, J. D., Brenner, P. F., Wilson, J. D., & Siiteri, P. K. (1979) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-49-6-905 · Cited by 318
Self-citationInduction of Human Endometrial Estradiol Dehydrogenase by Progestins
Tseng, L., & Gurpide, E. (1975) · Endocrinology · doi:10.1210/endo-97-4-825 · Cited by 301
Complete amino acid sequence of human placental 17β-hydroxysteroid dehydrogenase deduced from cDNA
Peltoketo, H., Isomaa, V., Mäentausta, O., & Vihko, R. (1988) · FEBS Letters · doi:10.1016/0014-5793(88)80548-9 · Cited by 294
Siiteri, P. K., & MacDonald, P. C. (1963) · Steroids · doi:10.1016/0039-128x(63)90044-8 · Cited by 244
Self-citationMartel, C., Rhéaume, E., Takahashi, M., Trudel, C., Couët, J., Luu-The, V., Simard, J., & Labrie, F. (1992) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(92)90390-5 · Cited by 231
Flagged first, then by citations
Research works printed but not linked: 1
- [4]4. Baird, D. T. 1976. Content and secretion of steroids by the human ovary in vivo. In Proceedings of the Fifth International Congress of Endocrinology. vol
Cited by
Sorted by citations
Items 201–213 of 213
denDekker, A. D. (2013) · ThinkIR: The University of Louisville's Institutional Repository (University of Louisville) · doi:10.18297/etd/330
The Syndrome of Apparent Mineralocorticoid Excess
White, P. C. (2009) · Elsevier eBooks · doi:10.1016/b978-0-12-449851-8.00017-6
Mature Female Reproductive System Endocrinology
Fazleabas, A. T., & Kim, J. (2002) · CRC Press eBooks · doi:10.3109/9780203219034-11
Modulation of the Androgen Receptor Axis
Mohler, M. L., & Dalton, J. T. (2021) · Wiley eBooks · doi:10.1002/0471266949.bmc055.pub2
Seyidova, C. I., & Yarmolinskaya, M. I. (2024) · Journal of obstetrics and women s diseases · doi:10.17816/jowd632714
Giton, F., Vartanian, A. D., Wyckens, N., Ferecatu, I., Chester, M., Héraud, C., Isik, A., Mathis, C., & Guigon, C. J. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2025.12.16.694587
Pocate-Cheriet, K. (2020) · theses.fr (ABES) · doi:10.70675/8a1fbb23z175dz4bbcz8427z6f4af93f23d1
Engineering the human endometrium at the intersection of development and reproduction
Vélez-Avilés, A. N., Abel, A., Taylor, H. S., & Sözen, B. (2026) · Nature Biomedical Engineering · doi:10.1038/s41551-026-01789-2
Shawar, L., Piper, T., & Eiler, J. M. (2026) · Analytical Chemistry · doi:10.1021/acs.analchem.6c03178
Francone, N. O., Bulun, S. E., Ma, Y., Yilmaz, B., Akin-Olugbade, O. O., Bernardi, L. A., Jungheim, E. S., Feinberg, E. C., Boots, C. E., PhD, M. K., et al. (2026) · Reproductive BioMedicine Online · doi:10.1016/j.rbmo.2026.105982
Metadata
- Authors
- Casey, M. L., MacDonald, P. C., & Andersson, S.
- Affiliations
- The University of Texas Southwestern Medical Center (US)
- Merck & Co., Inc., Rahway, NJ, USA (United States) (US)
- Venue
- Journal of Clinical Investigation
- Published
- 1994-11-01
- Volume
- 94
- Issue
- 5
- Pages
- 2135-2141
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC294662
- OpenAlex
- W2053311013
- Semantic Scholar
- e0c45caf9b177e26120a97b0a2082feb33ac7b43
- Cited by
- 212
- References
- 46
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 3 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 5 · 2019: 6 · 2018: 9 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 3
- FWCI
- 5.90
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5p50hd011149-17)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (5-P50-HD-11149)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- chromosomal localization, endometrium, luteal phase, progesterone regulation, gene expression, trophoblast, endoplasmic reticulum, glandular epithelium, stromal cells
- MeSH terms
- Chromosome Mapping, Chromosomes, Human, Pair 16, Endometrium, Female, Gene Expression Regulation, Humans, Isoenzymes, Placenta, Progestins, RNA, Messenger, 17-Hydroxysteroid Dehydrogenases
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
