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17 beta-Hydroxysteroid dehydrogenase type 2: chromosomal assignment and progestin regulation of gene expression in human endometrium.
Open accessAbstract
The cDNAs for two separate human 17 beta-hydroxysteroid dehydrogenases (17 beta-HSD) have been isolated and sequenced. The well-studied human placental cytosolic 17 beta-HSD (also referred to as estradiol dehydrogenase) preferentially catalyzes the reduction of estrone to estradiol-17 beta and the reduction of the C-20-ketone of progesterone to 20 alpha-dihydroprogesterone. This isoform of the enzyme has been referred to as 17 beta-HSD type 1 and localized to chromosome 17. A second 17 beta-HSD isoform (referred to as type 2) is localized in the endoplasmic reticulum of human trophoblast and is characterized by the preferential oxidation of the C-17 beta-hydroxyl group of C18- and C19-steroids and the C-20 alpha-hydroxyl group of 20 alpha-dihydroprogesterone. In this study, we determined the chromosomal localization of human 17 beta-HSD type 2, the expression of this gene in human endometrium, and the tissue distribution of the mRNA. We found that the human 17 beta-HSD type 2 gene is localized on chromosome 16, 16q24. 17 beta-HSD type 2 mRNA (approximately 1.5 kb) was identified in human endometrial tissues by Northern analysis of total RNA (10 micrograms). The highest levels of 17 beta-HSD type 2 mRNA were found in endometrial tissues obtained during the mid- to late secretory phase of the ovarian cycle (i.e., during the time of high plasma levels of progesterone). 17 beta-HSD type 2 mRNA levels were much greater in glandular epithelium than in the stromal cells isolated from secretory phase endometrium. The levels of 17 beta-HSD type 2 mRNA in secretory phase endometrium were approximately one-tenth that in villous trophoblast tissue from human placenta. We did not detect 17 beta-HSD type 1 mRNA in endometrial tissue by Northern analysis of total (10 micrograms) RNA. These findings are consistent with the view that the progestin-regulated 17 beta-HSD of the glandular epithelium of the human endometrium is primarily, if not exclusively, the product of the 17 beta-HSD type 2 gene. 17 beta-HSD type 2 mRNA was present in human placenta, liver, and small intestine; much smaller amounts, barely detectable by Northern analysis of poly(A)+ RNA, were present in prostate, kidney, pancreas, and colon, but not in heart, brain, skeletal muscle, spleen, thymus, ovary, or testis.
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“Progesterone via PGR induces retinoic acid production in endometrial stromal cells, which then acts on epithelial cells to induce HSD17B2 expression”
From Endometriosis and nuclear receptors (2019) · cites this paper as Casey et al., 1994
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“Instead, a cDNA encoding microsomal HSD17B2 was cloned (513) and found to be expressed in endometrium, placenta, and other tissues (514).”
From The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders (2011) · cites this paper as [514]
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Cites
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,460
High-Resolution Mapping of Human Chromosome 11 by in Situ Hybridization with Cosmid Clones
Lichter, P., Tang, C.-J. C., Call, K. M., Hermanson, G., Evans, G. A., Housman, D. E., & Ward, D. C. (1990) · Science · doi:10.1126/science.2294592 · Cited by 1,330
Placental Estrogen Biosynthesis During Human Pregnancy1
SIITERI, P. K., & MACDONALD, P. C. (1966) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-26-7-751 · Cited by 479
Self-citationWu, L., Einstein, M., Geissler, W. M., Chan, H. K., Elliston, K. O., & Andersson, S. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)31480-7 · Cited by 437
Self-citationOrigin of Estrogen in Normal Men and in Women with Testicular Feminization*
MacDonald, P. C., Madden, J. D., Brenner, P. F., Wilson, J. D., & Siiteri, P. K. (1979) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-49-6-905 · Cited by 318
Self-citationInduction of Human Endometrial Estradiol Dehydrogenase by Progestins
Tseng, L., & Gurpide, E. (1975) · Endocrinology · doi:10.1210/endo-97-4-825 · Cited by 301
Complete amino acid sequence of human placental 17β-hydroxysteroid dehydrogenase deduced from cDNA
Peltoketo, H., Isomaa, V., Mäentausta, O., & Vihko, R. (1988) · FEBS Letters · doi:10.1016/0014-5793(88)80548-9 · Cited by 294
Siiteri, P. K., & MacDonald, P. C. (1963) · Steroids · doi:10.1016/0039-128x(63)90044-8 · Cited by 244
Self-citationMartel, C., Rhéaume, E., Takahashi, M., Trudel, C., Couët, J., Luu-The, V., Simard, J., & Labrie, F. (1992) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(92)90390-5 · Cited by 231
Wevrick, R., & Willard, H. F. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.23.9394 · Cited by 229
Structure of two in tandem human 17β-hydroxysteroid dehydrogenase genes
Luu-The, V., Labrie, C., Simard, J., Lachance, Y., Zhao, H.-F., Couët, J., Leblanc, G., & Labrie, F. (1990) · Molecular Endocrinology · doi:10.1210/mend-4-2-268 · Cited by 173
Weinstein, R. L., Kelch, R. P., Jenner, M. R., Kaplan, S. L., & Grumbach, M. M. (1974) · Journal of Clinical Investigation · doi:10.1172/jci107526 · Cited by 170
Regulation of interleukin-8 gene expression in human endometrial cells in culture
Arici, A. M., Head, J. R., MacDonald, P. C., & Casey, M. L. (1993) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(93)90168-j · Cited by 157
Self-citationRegulation of the gene for estrogenic 17-ketosteroid reductase lying on chromosome 17cen–q25.
Tremblay, Y., Ringler, G. E., Morel, Y., Mohandas, T. K., Labrie, F., Strauss, J. F., & Miller, W. L. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)47083-x · Cited by 97
ESTRADIOL RECEPTOR AND 17β‐DEHYDROGENASE IN NORMAL AND ABNORMAL HUMAN ENDOMETRIUM*
Tseng, L., Gusberg, S. B., & Gurpide, E. (1977) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1977.tb29416.x · Cited by 97
Labrie, F., Simard, J., Luu-The, V., Pelletier, G., Bélanger, A., Lachance, Y., Zhao, H. F., Labrie, C., Breton, N., de Launoit, Y., et al. (1992) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(92)90368-s · Cited by 88
Lloyd, C. W., Lobotsky, J., Baird, D. T., McCracken, J. A., Weisz, J., PUPKIN, M. J., Zañartu, J., & PUGA, J. (1971) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-32-2-155 · Cited by 79
Estradiol binding and metabolism in human endometrial hyperplasia and adenocarcinoma
Gurpide, E., Gusberg, S. B., & Tseng, L. (1976) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(76)90007-8 · Cited by 76
Casey, M. L., Smith, J. W., Nagai, K., Hersh, L. B., & MacDonald, P. C. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54460-4 · Cited by 74
Self-citationEstrogen metabolism in normal and neoplastic endometrium
Gurpide, E., Tseng, L., & Gusberg, S. B. (1977) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(77)90401-x · Cited by 71
Dynamics of Uptake of Estrogens and Androgens by Human Endometrium
Gurpide, E., & Welch, M. T. (1969) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)63641-5 · Cited by 70
SATYASWAROOP, P. G., WARTELL, D. J., & Mortel, R. (1982) · Endocrinology · doi:10.1210/endo-111-3-743 · Cited by 66
Tseng, L., & Gurpide, E. (1979) · Endocrinology · doi:10.1210/endo-104-6-1745 · Cited by 65
Couture, P., Thériault, C., Simard, J., & Labrie, F. (1993) · Endocrinology · doi:10.1210/endo.132.1.8380373 · Cited by 54
POUTANEN, M. H., ISOMAA, V. V., Kainulainen, K., & Vihko, R. (1990) · International Journal of Cancer · doi:10.1002/ijc.2910460524 · Cited by 48
Blomquist, C. H., Lindemann, N. J., & Hakanson, E. Y. (1985) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(85)90828-8 · Cited by 46
Gast, M. J., Sims, H. F., Murdock, G. L., Gast, P. M., & Strauss, A. W. (1989) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(89)90958-7 · Cited by 45
Pack, B. A., TOVAR, R., Booth, E., & BROOKSf, S. C. (1979) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-48-3-420 · Cited by 45
Chapdelaine, A., MacDonald, P. C., GONZALEZ, O., Gurpide, E., Wiele, R. L. V., & Lieberman, S. (1965) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-25-12-1569 · Cited by 42
Self-citationStrickler, R. C., & Tobias, B. (1980) · Steroids · doi:10.1016/0039-128x(80)90022-7 · Cited by 39
Localization of estradiol 17β dehydrogenase in human endometrium
SCUBLINSKY, A., Marin, C., & Gurpide, E. (1976) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(76)90174-6 · Cited by 34
STUDIES ON THE METABOLISM OF C-21 STEROIDS IN THE HUMAN FOETO-PLANCENTAL UNIT
Palmer, R., Blair, J. A., Eriksson, G., & Diczfalusy, E. R. (1966) · European Journal of Endocrinology · doi:10.1530/acta.0.0530407 · Cited by 34
In vivo aspects of progesterone distribution and metabolism
Little, B., Billiar, R. B., Rahman, S. S., Johnson, W. A., Takaoka, Y., & White, R. J. (1975) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(75)90040-x · Cited by 32
MacDonald, P. C., Chapdelaine, A., GONZALEZ, O., Gurpide, E., Wiele, R. L. V., & Lieberman, S. (1965) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-25-12-1557 · Cited by 32
Self-citationEstrogen receptors in epithelial and stromal cells of human endometrium in culture
Fleming, H., Namit, C., & Gurpide, E. (1980) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(80)90266-6 · Cited by 29
Winqvist, R., Peltoketo, H., ISOMAA, V. V., Grzeschik, K., Mannermaa, A., & Vihko, R. (1990) · Human Genetics · doi:10.1007/bf00194219 · Cited by 26
Progesterone metabolism by proliferative and secretory human endometrium
Collins, J. A., & Jewkes, D. M. (1974) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(74)90546-8 · Cited by 24
STUDIES ON THE METABOLISM OF C-19 STEROIDS IN THE HUMAN FOETO-PLACENTAL UNIT
Benagiano, G., Kincl, F. A., Zielske, F., Wiqvist, N., & Diczfalusy, E. R. (1967) · European Journal of Endocrinology · doi:10.1530/acta.0.0560203 · Cited by 23
Palmer, R., Blair, J. A., Eriksson, G., et al. (1966) · PubMed · Cited by 22
Byrjalsen, I., Thormann, L., Meinecke, B., Riis, B. J., & Christiansen, C. (1992) · PubMed · Cited by 19
Hormone-related enzymatic activities in normal and cancer cells of human endometrium
Holinka, C. F., & Gurpide, E. (1981) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(81)90274-0 · Cited by 19
Apparent immunologic nonidentity of human placental and endometrial 17β-estradiol dehydrogenase
Marovitz, W. F., Loucopoulos, A., SATYASWAROOP, P. G., Gurpide, E., Todd, R., & Naftolin, F. N. (1980) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(80)90081-2 · Cited by 19
THE SUBCELLULAR DISTRIBUTION OF 17β-HYDROXYSTEROID DEHYDROGENASE IN THE HUMAN TERM PLACENTA
Thomas, C. M. G., & Veerkamp, J. H. (1976) · European Journal of Endocrinology · doi:10.1530/acta.0.0820150 · Cited by 13
Induction of estradiol dehydrogenase activity in human uterine endometrium by synthetic steroids
Kitawaki, J., Yamamoto, T., & Okada, H. (1988) · Journal of Endocrinological Investigation · doi:10.1007/bf03349053 · Cited by 11
Steroid secretory characteristics of a virilizing adrenal adenoma in a woman
Bradshaw, K. D., Milewich, L., Mason, J. I., Parker, C. R., MacDonald, P. C., & Carr, B. R. (1994) · Journal of Endocrinology · doi:10.1677/joe.0.1400297 · Cited by 8
Self-citationLloyd, C. W., Lobotsky, J., BAIED, D. T., McCRACKEN, J. A., Weisz, J., PUPKIN, M. J., Zañartu, J., & PUGA, J. (1971) · Obstetrical & Gynecological Survey · doi:10.1097/00006254-197109000-00018 · Cited by 4
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Research works printed but not linked: 1
- [4]4. Baird, D. T. 1976. Content and secretion of steroids by the human ovary in vivo. In Proceedings of the Fifth International Congress of Endocrinology. vol
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Sorted by citations
The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders
Miller, W. L., & Auchus, R. J. (2011) · Endocrine Reviews · doi:10.1210/er.2010-0013 · Cited by 2,458
Bulun, S. E. (2009) · New England Journal of Medicine · doi:10.1056/nejmra0804690 · Cited by 1,772
Functional role of estrogen metabolism in target cells: review and perspectives
Zhu, B. T. (1998) · Carcinogenesis · doi:10.1093/carcin/19.1.1 · Cited by 970
Bulun, S. E., Yilmaz, B. D., Sison, C., Miyazaki, K., Bernardi, L., Liu, S., Kohlmeier, A., Yin, P., Milad, M., & Wei, J. (2019) · Endocrine Reviews · doi:10.1210/er.2018-00242 · Cited by 844
Progesterone action in endometrial cancer, endometriosis, uterine fibroids, and breast cancer
Kim, J. J., Kurita, T., & Bulun, S. E. (2013) · Endocrine Reviews · doi:10.1210/er.2012-1043 · Cited by 520
Metadata
- Authors
- Casey, M. L., MacDonald, P. C., & Andersson, S.
- Affiliations
- The University of Texas Southwestern Medical Center (US)
- Merck & Co., Inc., Rahway, NJ, USA (United States) (US)
- Venue
- Journal of Clinical Investigation
- Published
- 1994-11-01
- Volume
- 94
- Issue
- 5
- Pages
- 2135-2141
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC294662
- OpenAlex
- W2053311013
- Semantic Scholar
- e0c45caf9b177e26120a97b0a2082feb33ac7b43
- Cited by
- 212
- References
- 46
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 3 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 5 · 2019: 6 · 2018: 9 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 3
- FWCI
- 5.90
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5p50hd011149-17)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (5-P50-HD-11149)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Physical Sciences › Chemistry › Organic Chemistry › Steroid Chemistry and Biochemistry
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- chromosomal localization, endometrium, luteal phase, progesterone regulation, gene expression, trophoblast, endoplasmic reticulum, glandular epithelium, stromal cells
- MeSH terms
- Chromosome Mapping, Chromosomes, Human, Pair 16, Endometrium, Female, Gene Expression Regulation, Humans, Isoenzymes, Placenta, Progestins, RNA, Messenger, 17-Hydroxysteroid Dehydrogenases
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