The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Bactericidal properties of murine intestinal phospholipase A2.
Open accessAbstract
We purified a molecule from the murine small intestine that killed both Escherichia coli and Listeria monocytogenes, and identified it as intestinal phospholipase A2 (iPLA2) by NH2-terminal sequencing and enzymatic measurements. The ability of iPLA2 to kill. L. monocytogenes was greatly enhanced by 5 mM calcium, inhibited by EGTA and abolished after reduction and alkylation, suggesting that enzymatic activity was required for iPLA2-mediated bactericidal activity. A mouse-avirulent phoP mutant, S. typhimurium 7953S, was 3.5-fold more susceptible to iPLA2 than its isogenic virulent parent, S. typhimurium 14028S (estimated minimal bactericidal concentrations 12.7 +/- 0.5 micrograms/ml vs. 43.9 +/- 4.5 micrograms/ml P < 0.001). Overall, these findings identify iPLA2 as part of the antimicrobial arsenal that equips Paneth cells to protect the small intestinal crypts from microbial invasion. Because iPLA2 is identical to Type 2 phospholipase A2 molecules found in other sites, including spleen, platelets and inflammatory exudate cells, this enzyme may also contribute to antibacterial defenses elsewhere in the body.
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Self-citationStructure and Properties of a Human Non-pancreatic Phospholipase A2
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Cited by
Paneth cells, antimicrobial peptides and maintenance of intestinal homeostasis
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Biochemistry and Physiology of Mammalian Secreted Phospholipases A2
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Metadata
- Authors
- Harwig, S. S., Tan, L., Qu, X. D., Cho, Y., Eisenhauer, P. B., & Lehrer, R. I.
- Venue
- Journal of Clinical Investigation
- Published
- 1995-02-01
- Volume
- 95
- Issue
- 2
- Pages
- 603-610
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W1992641427
- Cited by
- 254
- References
- 34
- FWCI
- 10.61
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Harwig, S. S., Tan, L., Qu, X. D., Cho, Y., Eisenhauer, P. B., & Lehrer, R. I. (1995). Bactericidal properties of murine intestinal phospholipase A2. Journal of Clinical Investigation. https://doi.org/10.1172/jci117704
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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