Cited by 425Pancreatic function and diabetesDiabetes Treatment and ManagementMetabolism, Diabetes, and Cancer

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Human and rat beta cells differ in glucose transporter but not in glucokinase gene expression.

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De Vos, A, Heimberg, H, Quartier, E, Huypens, P, Bouwens, L, Pipeleers, D, Schuit, F·1995·Journal of Clinical Investigation

DOI 10.1172/jci118308PMC PMC185903

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Abstract

Glucose homeostasis is controlled by a glucose sensor in pancreatic beta-cells. Studies on rodent beta-cells have suggested a role for GLUT2 and glucokinase in this control function and in mechanisms leading to diabetes. Little direct evidence exists so far to implicate these two proteins in glucose recognition by human beta-cells. The present in vitro study investigates the role of glucose transport and phosphorylation in beta-cell preparations from nondiabetic human pancreata. Human beta-cells differ from rodent beta-cells in glucose transporter gene expression (predominantly GLUT1 instead of GLUT2), explaining their low Km (3 mmol/liter) and low VMAX (3 mmol/min per liter) for 3-O-methyl glucose transport. The 100-fold lower GLUT2 abundance in human versus rat beta-cells is associated with a 10-fold slower uptake of alloxan, explaining their resistance to this rodent diabetogenic agent. Human and rat beta-cells exhibit comparable glucokinase expression with similar flux-generating influence on total glucose utilization. These data underline the importance of glucokinase but not of GLUT2 in the glucose sensor of human beta-cells.

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Jungermann, K. A., & Katz, N. R. (1989) · Physiological Reviews · doi:10.1152/physrev.1989.69.3.708 · Cited by 655
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Prolonged exposure of human pancreatic islets to high glucose concentrations in vitro impairs the beta-cell function.
Eizirik, D. L., Korbutt, G. S., & Hellerström, C. (1992) · Journal of Clinical Investigation · doi:10.1172/jci115989 · Cited by 313
Expression of human glucose transporters in Xenopus oocytes: kinetic characterization and substrate specificities of the erythrocyte, liver, and brain isoforms
Gould, G. W., THOMAS, H. M., Jess, T. J., & Bell, G. I. (1991) · Biochemistry · doi:10.1021/bi00235a004 · Cited by 303
Glucokinase as pancreatic beta cell glucose sensor and diabetes gene.
Matschinsky, F. M., Liang, Y. T., Kesavan, P., Wang, L., Froguel, P., Velho, G., Cohen, D., Permutt, M. A., Tanizawa, Y., & Jetton, T. L. (1993) · Journal of Clinical Investigation · doi:10.1172/jci116809 · Cited by 287
Diabetic Hyperglycemia: Link to Impaired Glucose Transport in Pancreatic β Cells
Unger, R. H. (1991) · Science · doi:10.1126/science.2006409 · Cited by 263
Major species differences between humans and rodents in the susceptibility to pancreatic beta-cell injury.
Eizirik, D. L., PIPELEERS, D. G., Ling, Z., Welsh, N., Hellerström, C., & Andersson, A. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.20.9253 · Cited by 261
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Johnson, J. H., Newgard, C. B., Milburn, J. L., Lodish, H. F., & Thorens, B. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39181-1 · Cited by 207
Primary pancreatic beta-cell secretory defect caused by mutations in glucokinase gene in kindreds of maturity onset diabetes of the young
Velho, G. (1992) · The Lancet · doi:10.1016/0140-6736(92)91768-4 · Cited by 202
Underexpression of β Cell High K m Glucose Transporters in Noninsulin-Dependent Diabetes
Johnson, J. H., Ogawa, A., Chen, L., Orci, L., Newgard, C. B., Alam, T., & Unger, R. H. (1990) · Science · doi:10.1126/science.2237405 · Cited by 201
Differences in Glucose Transporter Gene Expression between Rat Pancreatic α- and β-Cells Are Correlated to Differences in Glucose Transport but Not in Glucose Utilization
Heimberg, H., De Vos, A., Pipeleers, D., Thorens, B., & Schuit, F. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.15.8971 · Cited by 188
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Heimberg, H., Vos, A. D., Vandercammen, A., SCHAFTINGEN, E. V., PIPELEERS, D. G., & Schuit, F. C. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb05949.x · Cited by 167
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Roles of insulin resistance and beta-cell dysfunction in dexamethasone-induced diabetes.
Ogawa, A., Johnson, J. H., Ohneda, M., McAllister, C. T., Inman, L., Alam, T., & Unger, R. H. (1992) · Journal of Clinical Investigation · doi:10.1172/jci115886 · Cited by 159
Transfection of AtT-20ins cells with GLUT-2 but not GLUT-1 confers glucose-stimulated insulin secretion. Relationship to glucose metabolism
Hughes, S., Quaade, C., Johnson, J. H., Ferber, S., & Newgard, C. B. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)82457-7 · Cited by 154
Elusive Proximal Signals of β-Cells for Insulin Secretion
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Differential localization of two glucose transporter isoforms in rat kidney
Thorens, B., Lodish, H. F., & Brown, D. (1990) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1990.259.2.c286 · Cited by 141
Ribozyme-mediated attenuation of pancreatic beta-cell glucokinase expression in transgenic mice results in impaired glucose-induced insulin secretion.
Efrat, S., Leiser, M., Wu, Y. J., Fusco-DeMane, D., al Emran, O., Surana, M., Jetton, T. L., Magnuson, M. A., Weir, G. C., & Fleischer, N. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.6.2051 · Cited by 124
Kinetic properties of the reconstituted glucose transporter from human erythrocytes.
Wheeler, T. J., & Hinkle, P. C. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52485-1 · Cited by 120
Regulation of Glucose Metabolism in Pancreatic Islets
Trus, M. D., Zawalich, W. S., Burch, P. T., Berner, D. K., Weill, V. A., & Matschinsky, F. M. (1981) · Diabetes · doi:10.2337/diab.30.11.911 · Cited by 114
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Engineering of glucose-stimulated insulin secretion and biosynthesis in non-islet cells.
Hughes, S., Johnson, J. H., Quaade, C., & Newgard, C. B. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.2.688 · Cited by 108
A mutation in the Glut2 glucose transporter gene of a diabetic patient abolishes transport activity.
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Reduced beta-cell glucose transporter in new onset diabetic BB rats.
Orci, L., Unger, R. H., Ravazzola, M., Ogawa, A., Komiya, I., Baetens, D., Lodish, H. F., & Thorens, B. (1990) · Journal of Clinical Investigation · doi:10.1172/jci114883 · Cited by 96
Differences in glucose handling by pancreatic A- and B-cells.
Gorus, F., Malaisse, W. J., & PIPELEERS, D. G. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)43587-3 · Cited by 95
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Slieker, L. J., Sundell, K. L., Heath, W. F., Osborne, H. E., Bue, J., Manetta, J., & Sportsman, J. R. (1992) · Diabetes · doi:10.2337/diab.41.2.187 · Cited by 90
Cloning and functional expression of a human pancreatic islet glucose-transporter cDNA.
Permutt, M. A., Koranyi, L. I., Keller, K., Lacy, P. E., Scharp, D. W., & Mueckler, M. M. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.22.8688 · Cited by 89
Tissue-specific expression of glucokinase: identification of the gene product in liver and pancreatic islets.
Iynedjian, P. B., Möbius, G., Seitz, H. J., Wollheim, C. B., & Renold, A. E. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.7.1998 · Cited by 87
Expression of GLUT-2 antisense RNA in beta cells of transgenic mice leads to diabetes.
Valera, A., Solanes, G., Fernández-Álvarez, J., Pujol, A., Ferrer, J. P., Asins, G., Gomis, R., & Bosch, F. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)61937-x · Cited by 85
Mechanisms of glucose stimulated insulin secretion in health and in diabetes: Some re-evaluations and proposals
Cerasi, E. (1975) · Diabetologia · doi:10.1007/bf00422811 · Cited by 75
Alloxan Uptake by Isolated Rat Islets of Langerhans*
Weaver, D. C., McDaniel, M. L., & Lacy, P. E. (1978) · Endocrinology · doi:10.1210/endo-102-6-1847 · Cited by 74
Expression and function of GLUT-1 and GLUT-2 glucose transporter isoforms in cells of cultured rat pancreatic islets.
Tal, M., Liang, Y. T., Najafi, H., Lodish, H. F., & Matschinsky, F. M. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)41918-7 · Cited by 73
Glucokinase Gene is Genetic Marker for NIDDM in American Blacks
Chiu, K. C., Province, M. A., & Permutt, M. A. (1992) · Diabetes · doi:10.2337/diab.41.7.843 · Cited by 70
The Glucokinase Glucose Sensor in Human Pancreatic Islet Tissue
Bedoya, F. J., Wilson, J. M., Ghosh, A. K., Finegold, D. N., & Matschinsky, F. M. (1986) · Diabetes · doi:10.2337/diab.35.1.61 · Cited by 69
Expression of the brain-type glucose transporter is restricted to brain and neuronal cells in mice
Gould, G. W., Brant, A. M., Kahn, B. B., Shepherd, P. R., McCoid, S. C., & Gibbs, E. M. (1992) · Diabetologia · doi:10.1007/bf00401196 · Cited by 52
Loss of glucose-induced insulin secretion and GLUT2 expression in transplanted β-cells
Ogawa, Y., Noma, Y., Davalli, A. M., Wu, Y., Thorens, B., Bonner‐Weir, S., & Weir, G. C. (1995) · Diabetes · doi:10.2337/diab.44.1.75 · Cited by 34
Regulation of glucose metabolism in pancreatic islets
Trus, M. D., Zawalich, W. S., Burch, P. T., et al. (1981) · Diabetes · doi:10.2337/diabetes.30.11.911 · Cited by 31
Glucokinase gene is genetic marker for NIDDM in American blacks
Chiu, K. C., Province, M. A., & Permutt, M. A. (1992) · Diabetes · doi:10.2337/diabetes.41.7.843 · Cited by 31
Abnormal facilitative glucose transporter gene expression in human islet cell tumors
Seino, Y., Yamamoto, T. T., Inoue, K., Imamura, M., Kadowaki, S., Kojima, H., Fujikawa, J., & Imura, H. (1993) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.76.1.8421107 · Cited by 26
Linkage Analysis of GLUT1 (HepG2) and GLUT2 (Liver/Islet) Genes in Familial NIDDM
Elbein, S. C., Hoffman, M. D., Matsutani, A., & Permutt, M. A. (1992) · Diabetes · doi:10.2337/diab.41.12.1660 · Cited by 23
The glucokinase glucose sensor in human pancreatic islet tissue
Bedoya, F. J., Wilson, J. M., Ghosh, A. K., et al. (1986) · Diabetes · doi:10.2337/diabetes.35.1.61 · Cited by 17
CHRONIC EXPOSURE OF HUMAN PANCREATIC BETA-CELLS TO HIGH GLUCOSE INCREASES THEIR FUNCTIONAL-ACTIVITY BUT DECREASES THEIR SENSITIVITY TO ACUTE REGULATION BY GLUCOSE
Ling, Z., Proft, R. D., & PIPELEERS, D. G. (1993) · VUBIR (Vrije Universiteit Brussel) · Cited by 2
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Research works printed but not linked: 2

[9]9. Efrat, S., M. Leiser, Y. J. Wu, D. Fusco-DeMane, O. A. Emran, M. Surana, T. L. Jetton, M. A. Magnuson, G. Weir, and N. Fleischer. 1994. Ribozymemudao ss suss ss eunc mice results in impaired glucose-induced insulin secretion. Proc. Natl. Acad. Sci. USA. 91:2051–2055.
[17]17. Pipeleers, D. 1994. Treatment of diabetes by islet cell transplantation. In Biomedical and Health Research. Vol. 3, Advances in Medical Biology. C. Baya, editor. IOS Press, Amsterdam. 185–197.

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Authors: De Vos, A., Heimberg, H., Quartier, E., Huypens, P., Bouwens, L., Pipeleers, D., & Schuit, F.
ORCID: Heimberg, H
Quartier, E
Huypens, P
Bouwens, L
Pipeleers, D
Schuit, F
Venue: Journal of Clinical Investigation
Publisher: American Society for Clinical Investigation
Published: 1995-11-01
Volume: 96
Issue: 5
Pages: 2489-2495
ISSN: 0021-9738 (ISSN-L)
DOI: 10.1172/jci118308
PMC: PMC185903
OpenAlex: W2015001567
Cited by: 425
References: 40
Citations by year: 2026: 3 · 2025: 13 · 2024: 16 · 2023: 16 · 2022: 15 · 2021: 23 · 2020: 14 · 2019: 15 · 2018: 12 · 2017: 17 · 2016: 27 · 2015: 21 · 2014: 23 · 2013: 23 · 2012: 21
FWCI: 11.19
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Journal of Clinical Investigation (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Eli Lilly and Company
Universitaire Stichting
Université de Genève
Topics: Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Keywords: pancreatic beta cells, GLUT2, GLUT1, glucokinase, glucose transporter gene expression, glucose detection, glucose homeostasis, alloxan, glucose phosphorylation, diabetes mellitus
MeSH terms: Animals, Cells, Cultured, Glucokinase, Glucose, Humans, Islets of Langerhans, Monosaccharide Transport Proteins, Phosphorylation, Gene Expression, Glucose Transporter Type 1, Glucose Transporter Type 2, Rats

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Human and rat beta cells differ in glucose transporter but not in glucokinase gene expression.

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