Dog mastocytoma cells secrete a 92-kD gelatinase activated extracellularly by mast cell chymase.
Open accessAbstract
Gelatinolytic metalloproteinases implicated in connective tissue remodeling and tumor invasion are secreted from several types of cells in the form of inactive zymogens. In this report, characterization of gelatinase activity secreted by the BR line of dog mastocytoma cells reveals a phorbol-inducible, approximately 92-kD, Ca2+ - and Zn2+ -dependent proenzyme cleaved over time to smaller, active forms. Incubation of cells with the general serine protease inhibitor, PMSF, prevented proenzyme cleavage and permitted its purification free of activation products. The NH2-terminal 13 amino acids of the purified mastocytoma progelatinase are 50-67% identical to those of human, mouse, and rabbit 92-kD progelatinase (gelatinase B; matrix metalloproteinase-9). Degranulation of mastocytoma cells using ionophore A23187 greatly accelerated proenzyme cleavage, suggesting that a serine protease present in secretory granules hydrolyzed the progelatinase to active fragments. To identify the activating protease, cells were coincubated with ionophore and a panel of selective serine protease inhibitors. Soybean trypsin inhibitor and succinyl-L-Ala-Ala-Pro-Phe-chloromethylketone, which inhibit mast cell chymase, prevented progelatinase activation. Inhibitors of tryptase and dog mast cell protease (dMCP)-3, i.e., aprotinin or bis(5-amidino-2-benzimidazolyl) methane (BABIM), did not. In further experiments using highly purified enzymes, mastocytoma cell chymase activated 92-kD progelatinase in the absence of other enzymes or cofactors; tryptase and dMCP-3, however, had no effect. These data demonstrate that dog mastocytoma cells secrete a metalloproteinase related to progelatinase B that is directly activated outside of the cell by exocytosed chymase, and provide the first demonstration of a cell that activates a matrix metalloproteinase it secretes by cosecreting an activating enzyme. In mastocytomas, this pathway may facilitate tumor invasion of surrounding tissues, and in normal mast cells, it could play a role in tissue remodeling and repair.
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Mast cell tryptases and chymases activate the precursors of MMP2, MMP9, collagenase and stromelysin.
“Moreover, MC tryptases and chymases have been shown to activate the precursors of MMP2 [12], MMP9 [13], collagenase and stromelysin [14].”
From Estradiol and Progesterone Regulate the Migration of Mast Cells from the Periphery to the Uterus and Induce Their Maturation and Degranulation (2010) · cites this paper as [13]
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Cites
A matrix metalloproteinase expressed on the surface of invasive tumour cells
Sato, H., Takino, T., Okada, Y., Cao, J. P., Shinagawa, A., Yamamoto, E., & Seiki, M. (1994) · Nature · doi:10.1038/370061a0 · Cited by 2,534 · see citing works
Metalloproteinases and their inhibitors in matrix remodeling
Matrisian, L. M. (1990) · Trends in Genetics · doi:10.1016/0168-9525(90)90126-q · Cited by 1,626 · see citing works
Van Wart, H. E., & Birkedal-Hansen, H. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.14.5578 · Cited by 1,475 · see citing works
Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase
Kjeldsen, L., Johnsen, A., Sengeløv, H., & Borregaard, N. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)82217-7 · Cited by 1,286 · see citing works
Wilhelm, S. M., Collier, I. E., Marmer, B. L., Eisen, A. Z., Grant, G. A., & Goldberg, G. I. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)71480-4 · Cited by 1,109 · see citing works
Tissue Inhibitor of Metalloproteinase (TIMP-2)
Stetler‐Stevenson, W. G., Krutzsch, H. C., & Liotta, L. A. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)71503-2 · Cited by 899 · see citing works
Springman, E. B., Angleton, E. L., Birkedal‐Hansen, H., & Wart, H. E. V. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.1.364 · Cited by 713 · see citing works
Ogata, Y., Enghild, J. J., & Nagase, H. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50563-4 · Cited by 633 · see citing works
Furin-dependent intracellular activation of the human stromelysin-3 zymogen
Pei, D., & Weiss, S. J. (1995) · Nature · doi:10.1038/375244a0 · Cited by 629 · see citing works
Biochemical and immunological characterization of the secreted forms of human neutrophil gelatinase.
Hibbs, M. S., Hasty, K. A., Seyer, J. M., Kang, A. H., & Mainardi, C. L. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)89580-1 · Cited by 613 · see citing works
Goldberg, G. I., Marmer, B. L., Grant, G. A., Eisen, A. Z., Wilhelm, S. M., & He, C. S. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.21.8207 · Cited by 586 · see citing works
Tissue cooperation in a proteolytic cascade activating human interstitial collagenase.
HE, C. S., Wilhelm, S. M., Pentland, A. P., Marmer, B. L., Grant, G. A., Eisen, A. Z., & Goldberg, G. I. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.8.2632 · Cited by 572 · see citing works
Human 92- and 72-kilodalton type IV collagenases are elastases.
Senior, R. M., Griffin, G. L., Fliszar, C. J., Shapiro, S. D., Goldberg, G. I., & Welgus, H. G. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)89530-1 · Cited by 544 · see citing works
Welgus, H. G., Campbell, E. J., Cury, J., Eisen, A. Z., Senior, R. M., Wilhelm, S. M., & Goldberg, G. I. (1990) · Journal of Clinical Investigation · doi:10.1172/jci114867 · Cited by 446 · see citing works
Mechanisms of activation of tissue procollagenase by matrix metalloproteinase 3 (stromelysin)
Suzuki, K., Enghild, J. J., MORODOMI, T., Salvesen, G. S., & Nagase, H. (1990) · Biochemistry · doi:10.1021/bi00496a016 · Cited by 432 · see citing works
Gruber, B. L., Marchese, M. J., Suzuki, K., Schwartz, L. B., Okada, Y., Nagase, H., & Ramamurthy, N. S. (1989) · Journal of Clinical Investigation · doi:10.1172/jci114344 · Cited by 346 · see citing works
Huhtala, P., Tuuttila, A., Chow, L. T., Lohi, J. J., Keski‐Oja, J., & Tryggvason, K. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55326-6 · Cited by 333 · see citing works
Saarinen, J., Kalkkinen, N., Welgus, H. G., & Kovanen, P. T. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)32427-4 · Cited by 313 · see citing works
Structure of the human type IV collagenase gene.
Huhtala, P., Chow, L. T., & Tryggvason, K. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)38559-x · Cited by 311 · see citing works
Ultrastructure of pulmonary mast cells in patients with fibrotic lung disorders.
Kawanami, O., Vj, F., Fulmer, J. D., & Rg, C. (1979) · PubMed · Cited by 265 · see citing works
Human mast cell tryptase activates single-chain urinary-type plasminogen activator (pro-urokinase)
Stack, M. S., & Johnson, D. A. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36896-5 · Cited by 237 · see citing works
Detergent-activation of latent collagenase and resolution of its component molecules
Birkedal‐Hansen, H., & TAYLOR, R. E. (1982) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(82)80120-4 · Cited by 197 · see citing works
LEES, M., TAYLOR, D. J., & WOOLLEY, D. E. (1994) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1994.tb18980.x · Cited by 178 · see citing works
Activation of the 92-kDa Gelatinase by Stromelysin and 4-Aminophenylmercuric Acetate
Shapiro, S. D., Fliszar, C. J., Broekelmann, T. J., Mecham, R. P., Senior, R. M., & Welgus, H. G. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.11.6351 · Cited by 164 · see citing works
Yang, T.-T., & Hawkes, S. P. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.22.10676 · Cited by 138 · see citing works
Mast Cells in Fibrotic Lung Disorders
Pesci, A., Bertorelli, G., Gabrielli, M., & Olivieri, D. (1993) · CHEST Journal · doi:10.1378/chest.103.4.989 · Cited by 135 · see citing works
Kjeldsen, L., Bainton, D., Sengeløv, H., & Borregaard, N. (1993) · Blood · doi:10.1182/blood.v82.10.3183.3183 · Cited by 134 · see citing works
Fini, M. E., Bartlett, J. D., Matsubara, M., Rinehart, W. B., Mody, M. K., Girard, M. T., & Rainville, M. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)61950-2 · Cited by 109 · see citing works
Kjeldsen, L., Bainton, D., Sengeløv, H., et al. (1993) · Blood · doi:10.1182/blood.v82.10.3183.bloodjournal82103183 · Cited by 107 · see citing works
Molecular Cloning and Expression of the Mouse 105-kDa Gelatinase cDNA
Tanaka, H., Hojo, K., Yoshida, H., Yoshioka, T., & Sugita, K. (1993) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1993.1110 · Cited by 91 · see citing works
Mast cell proteases in immunology and biology
Caughey, G. H. (1995) · M. Dekker eBooks · Cited by 79 · see citing works
Self-citationDog mastocytoma tryptase: Affinity purification, characterization, and amino-terminal sequence
Caughey, G. H., Viro, N. F., Ramachandran, J., Lazarus, S. C., Borson, D. B., & Nadel, J. A. (1987) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(87)90377-8 · Cited by 75 · see citing works
Self-citationMast Cells' Contribution to the Fibrosing Alveolitis of the Scleroderma Lung
Chanez, P., Lacoste, J. Y., Guillot, B., Giron, J., Barneon, G., Enander, I., Godard, P., Michel, F. B., & Bousquet, J. (1993) · American Review of Respiratory Disease · doi:10.1164/ajrccm/147.6_pt_1.1497 · Cited by 70 · see citing works
Caughey, G. H., Raymond, W. W., Bacci, E., Lombardy, R. J., & Tidwell, R. R. (1993) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)10193-6 · Cited by 70 · see citing works
Self-citationHuman T lymphocytes synthesize the 92 kDa type IV collagenase (gelatinase B)
Weeks, B. S., Schnaper, H. W., Handy, M., Holloway, E., & Kleinman, H. K. (1993) · Journal of Cellular Physiology · doi:10.1002/jcp.1041570326 · Cited by 68 · see citing works
Arthritis and mast cell activation
Mican, J. M., & Metcalfe, D. D. (1990) · Journal of Allergy and Clinical Immunology · doi:10.1016/s0091-6749(05)80240-4 · Cited by 65 · see citing works
Caughey, G. H., Viro, N. F., Lazarus, S. C., & Nadel, J. A. (1988) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/0167-4838(88)90109-4 · Cited by 55 · see citing works
Self-citationNeutrophil Elastase Processing of Gelatinase A Is Mediated by Extracellular Matrix
Rice, A. G., & Banda, M. J. (1995) · Biochemistry · doi:10.1021/bi00028a038 · Cited by 42 · see citing works
Mookhtiar, K. A., Mallya, S. K., & Wart, H. E. V. (1986) · Analytical Biochemistry · doi:10.1016/0003-2697(86)90557-9 · Cited by 33 · see citing works
Purification and Characterization of Dog Mast Cell Protease-3, an Oligomeric Relative of Tryptases
Raymond, W. W., Tam, E. K., Blount, J. L., & Caughey, G. H. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.22.13164 · Cited by 21 · see citing works
Self-citation
Flagged first, then by citations
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Sorted by citations
Mast cell secretory granules: armed for battle
Wernersson, S., & Pejler, G. (2014) · Nature reviews. Immunology · doi:10.1038/nri3690 · Cited by 968 · see citing works
Inflammatory mast cells up-regulate angiogenesis during squamous epithelial carcinogenesis
Coussens, L. M., Raymond, W. W., Bergers, G., Laig-Webster, M., Behrendtsen, O., Werb, Z., Caughey, G. H., & Hanahan, D. (1999) · Genes & Development · doi:10.1101/gad.13.11.1382 · Cited by 946 · see citing works
Self-citationBiochemistry and Molecular Biology of Gelatinase B or Matrix Metalloproteinase-9 (MMP-9)
den Steen, P. E. V., Dubois, B., Nelissen, I., Rudd, P., Dwek, R., & Opdenakker, G. (2002) · Critical reviews in biochemistry and molecular biology · doi:10.1080/10409230290771546 · Cited by 948 · see citing works
da Silva, E. Z. M., Jamur, M. C., & Oliver, C. (2014) · Journal of Histochemistry & Cytochemistry · doi:10.1369/0022155414545334 · Cited by 607 · see citing works
Mast cell tryptases and chymases in inflammation and host defense.
Caughey, G. H. (2007) · Immunological Reviews · doi:10.1111/j.1600-065x.2007.00509.x · Cited by 442 · see citing works
Self-citationHough, K. P., Curtiss, M. L., Blain, T. J., Liu, R.-M., Trevor, J., Deshane, J. S., & Thannickal, V. J. (2020) · Frontiers in Medicine · doi:10.3389/fmed.2020.00191 · Cited by 432 · see citing works
Mast cells promote atherosclerosis by releasing proinflammatory cytokines
Sun, J., Sukhova, G. K., Wolters, P. J., Yang, M., Kitamoto, S., Libby, P., MacFarlane, L. A., Clair, J. M., & Shi, G. (2007) · Nature Medicine · doi:10.1038/nm1601 · Cited by 425 · see citing works
Mast cell proteases: multifaceted regulators of inflammatory disease
Pejler, G., Rönnberg, E., Waern, I., & Wernersson, S. (2010) · Blood · doi:10.1182/blood-2010-01-257287 · Cited by 378 · see citing works
Pejler, G., Åbrink, M., Ringvall, M., & Wernersson, S. (2007) · Advances in immunology · doi:10.1016/s0065-2776(07)95006-3 · Cited by 324 · see citing works
Cathepsin S Controls Angiogenesis and Tumor Growth via Matrix-derived Angiogenic Factors
Wang, B., Sun, J., Kitamoto, S., Yang, M., GRUBB, A. O., Chapman, H. A., Kalluri, R., & Shi, G. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m509134200 · Cited by 291 · see citing works
Mast cells modulate the pathogenesis of elastase-induced abdominal aortic aneurysms in mice
Sun, J., Sukhova, G. K., Yang, M., Wolters, P. J., MacFarlane, L. A., Libby, P., Sun, C., Zhang, Y., Liu, J., Ennis, T. L., et al. (2007) · Journal of Clinical Investigation · doi:10.1172/jci31311 · Cited by 248 · see citing works
Canine mast cell tumours: a review of the pathogenesis, clinical features, pathology and treatment
Welle, M. M., Bley, C. R., Howard, J., & Rüfenacht, S. (2008) · Veterinary Dermatology · doi:10.1111/j.1365-3164.2008.00694.x · Cited by 246 · see citing works
Baram, D., Vaday, G. G., Salamon, P., Drucker, I., Hershkoviz, R., & Mekori, Y. A. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.167.7.4008 · Cited by 233 · see citing works
Miller, H. R. P., & Pemberton, A. D. (2002) · Immunology · doi:10.1046/j.1365-2567.2002.01375.x · Cited by 230 · see citing works
Dipeptidyl Peptidase I Is Essential for Activation of Mast Cell Chymases, but Not Tryptases, in Mice
Wolters, P. J., Pham, C. T. N., Muilenburg, D. J., Ley, T. J., & Caughey, G. H. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m100223200 · Cited by 202 · see citing works
Self-citationFrangogiannis, N. G., Perrard, J. L., Mendoza, L. H., Burns, A. R., Lindsey, M. L., Ballantyne, C. M., Michael, L. H., Smith, C. W., & Entman, M. L. (1998) · Circulation · doi:10.1161/01.cir.98.7.687 · Cited by 178 · see citing works
Inflammation, proteases and cancer
van Kempen, L. C. L. T., de Visser, K. E., & Coussens, L. M. (2006) · European Journal of Cancer · doi:10.1016/j.ejca.2006.01.004 · Cited by 175 · see citing works
Tchougounova, E., Pejler, G., & Åbrink, M. (2003) · The Journal of Experimental Medicine · doi:10.1084/jem.20030671 · Cited by 174 · see citing works
Gelatinase B: Structure, Regulation, and Function
Vu, T. H., & Werb, Z. (1998) · Elsevier eBooks · doi:10.1016/b978-012545090-4/50006-9 · Cited by 174 · see citing works
Pan, J.-H., Sukhova, G. K., Yang, J.-T., Wang, B., Xie, T., Fu, H., Zhang, Y., Satoskar, A. R., David, J. R., Metz, C. N., et al. (2004) · Circulation · doi:10.1161/01.cir.0000134704.84454.d2 · Cited by 168 · see citing works
Self-citationMast Cell Proteases as Protective and Inflammatory Mediators
Caughey, G. H. (2011) · Advances in experimental medicine and biology · doi:10.1007/978-1-4419-9533-9_12 · Cited by 163 · see citing works
Self-citationMatrix metalloproteinases and their inhibitors in pulmonary hypertension
Chelladurai, P., Seeger, W., & Pullamsetti, S. S. (2012) · European Respiratory Journal · doi:10.1183/09031936.00209911 · Cited by 160 · see citing works
Fang, K. C., Raymond, W. W., Blount, J. L., & Caughey, G. H. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.41.25628 · Cited by 156 · see citing works
Self-citationCritical Role of Mast Cell Chymase in Mouse Abdominal Aortic Aneurysm Formation
Sun, J., Zhang, J., Lindholt, J. S., Sukhova, G. K., Liu, J., He, A., Åbrink, M., Pejler, G., Stevens, R. L., Thompson, R. W., et al. (2009) · Circulation · doi:10.1161/circulationaha.109.849679 · Cited by 153 · see citing works
Mast cell proteases as pharmacological targets
Caughey, G. H. (2015) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2015.04.045 · Cited by 151 · see citing works
Self-citationJensen, F., Woudwyk, M., Teles, A., Woidacki, K., Taran, F., Costa, S., Malfertheiner, S. F., & Zenclussen, A. C. (2010) · PLoS ONE · doi:10.1371/journal.pone.0014409 · Cited by 145 · see citing works
“Moreover, MC tryptases and chymases have been shown to activate the precursors of MMP2 [12], MMP9 [13], collagenase and stromelysin [14].”
BackgroundMast Cell Expression of Gelatinases A and B Is Regulated by kit Ligand and TGF-β
Fang, K. C., Wolters, P. J., Steinhoff, M. S., Bidgol, A., Blount, J. L., & Caughey, G. H. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.162.9.5528 · Cited by 134 · see citing works
Self-citationKitamoto, S., Sukhova, G. K., Sun, J., Yang, M., Libby, P., Love, V., Duramad, P., Sun, C., Zhang, Y., Yang, X. H., et al. (2007) · Circulation · doi:10.1161/circulationaha.107.688523 · Cited by 131 · see citing works
Hara, M., Matsumori, A., Ono, K., Kido, H., Hwang, M., Miyamoto, T., Iwasaki, A., Okada, M., Nakatani, K., & Sasayama, S. (1999) · Circulation · doi:10.1161/01.cir.100.13.1443 · Cited by 117 · see citing works
Multifunctional Role of Chymase in Acute and Chronic Tissue Injury and Remodeling
Dell’Italia, L. J., Collawn, J. F., & Ferrario, C. M. (2018) · Circulation Research · doi:10.1161/circresaha.117.310978 · Cited by 113 · see citing works
Qin, Y., Cao, X., Guo, J., Zhang, Y., Pan, L., Zhang, H., Li, H., Tang, C., Du, J., & Shi, G. (2012) · Cardiovascular Research · doi:10.1093/cvr/cvs263 · Cited by 112 · see citing works
The Role of Mast Cell Specific Chymases and Tryptases in Tumor Angiogenesis
de Souza, D. A., Santana, A. C., da Silva, E. Z. M., Oliver, C., & Jamur, M. C. (2015) · BioMed Research International · doi:10.1155/2015/142359 · Cited by 106 · see citing works
Girolamo, N. D., Indoh, I., Jackson, N., Wakefield, D., McNeil, H. P., Yan, W., Geczy, C. L., Arm, J. P., & Tedla, N. T. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.177.4.2638 · Cited by 103 · see citing works
Serglycin proteoglycan is required for secretory granule integrity in mucosal mast cells
Braga, T., Grujić, M., Lukinius, A., Hellman, L., Åbrink, M., & Pejler, G. (2007) · Biochemical Journal · doi:10.1042/bj20061257 · Cited by 101 · see citing works
Cathepsin K Deficiency Reduces Elastase Perfusion–Induced Abdominal Aortic Aneurysms in Mice
Sun, J., Sukhova, G. K., Zhang, J., Chen, H., Sjöberg, S., Libby, P., Xia, M., Xiong, N., Gelb, B. D., & Shi, G. (2011) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.111.235002 · Cited by 100 · see citing works
Han, Y., Yan, C., Zhou, L., Lan, Q., & Tsukamoto, H. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m700554200 · Cited by 93 · see citing works
Frank, B. T., Rossall, J. C., Caughey, G. H., & Fang, K. C. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.166.4.2783 · Cited by 89 · see citing works
Self-citationOyamada, S., Bianchi, C. F., Takai, S., Chu, L. M., & Sellke, F. W. (2011) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.111.179697 · Cited by 88 · see citing works
Mast cell chymase: morphofunctional characteristics
Atiakshin, D., Buchwalow, I., & Tiemann, M. (2019) · Histochemistry and Cell Biology · doi:10.1007/s00418-019-01803-6 · Cited by 88 · see citing works
Rosario, H., Waldo, S. W., Becker, S. A., & Schmid‐Schönbein, G. W. (2004) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)63729-7 · Cited by 87 · see citing works
Regulation and Function of Mast Cell Proteases in Inflammation
Huang, C. B., Šali, A., & Stevens, R. L. (1998) · Journal of Clinical Immunology · doi:10.1023/a:1020574820797 · Cited by 86 · see citing works
Human Mast Cell β-Tryptase Is a Gelatinase
Fajardo, I., & Pejler, G. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.171.3.1493 · Cited by 83 · see citing works
A novel vascular smooth muscle chymase is upregulated in hypertensive rats
Guo, C., Ju, H., Leung, D., Massaeli, H., Shi, M., & Rabinovitch, M. (2001) · Journal of Clinical Investigation · doi:10.1172/jci9997 · Cited by 83 · see citing works
The emerging role of mast cell proteases in asthma
Pejler, G. (2019) · European Respiratory Journal · doi:10.1183/13993003.00685-2019 · Cited by 81 · see citing works
Cathepsin L Activity Is Essential to Elastase Perfusion–Induced Abdominal Aortic Aneurysms in Mice
Sun, J., Sukhova, G. K., Zhang, J., Chen, H., Sjöberg, S., Libby, P., Xiang, M., Wang, J., Peters, C., Reinheckel, T., et al. (2011) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.111.230201 · Cited by 80 · see citing works
Mast cells: new targets for multiple sclerosis therapy?
Zappulla, J. P., Arock, M., Mars, L. T., & Liblau, R. (2002) · Journal of Neuroimmunology · doi:10.1016/s0165-5728(02)00250-3 · Cited by 78 · see citing works
Novel Insight into the in vivo Function of Mast Cell Chymase: Lessons from Knockouts and Inhibitors
Pejler, G. (2020) · Journal of Innate Immunity · doi:10.1159/000506985 · Cited by 73 · see citing works
Mast Cells Play a Critical Role in the Pathogenesis of Viral Myocarditis
Higuchi, H., Hara, M., Yamamoto, K., Miyamoto, T., Kinoshita, M., Yamada, T., Uchiyama, K., & Matsumori, A. (2008) · Circulation · doi:10.1161/circulationaha.107.741595 · Cited by 70 · see citing works
Regulatory T cells in human and angiotensin II-induced mouse abdominal aortic aneurysms
Zhou, Y., Wu, W., Lindholt, J. S., Sukhova, G. K., Libby, P., Yu, X., & Shi, G. (2015) · Cardiovascular Research · doi:10.1093/cvr/cvv119 · Cited by 70 · see citing works
Han, Y., Nien, Y.-D., & Garner, W. L. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m202842200 · Cited by 69 · see citing works
Meyer, N., Woidacki, K., Knöfler, M., Meinhardt, G., Nowak, D., Velicky, P., Pollheimer, J., & Zenclussen, A. C. (2017) · Scientific Reports · doi:10.1038/srep45106 · Cited by 67 · see citing works
Tanaka, A., Arai, K., Kitamura, Y., & Matsuda, H. (1999) · Blood · doi:10.1182/blood.v94.7.2390.419k16_2390_2395 · Cited by 67 · see citing works
Mast cell chymase contributes to the antibody response and the severity of autoimmune arthritis
Magnusson, S. E., Pejler, G., Kleinau, S., & Åbrink, M. (2008) · The FASEB Journal · doi:10.1096/fj.08-120394 · Cited by 62 · see citing works
Qin, Y., & Shi, G. (2011) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2011.04.010 · Cited by 60 · see citing works
Levick, S. P., Gardner, J. D., Holland, M., Hauer‐Jensen, M., Janicki, J. S., & Brower, G. L. (2008) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2008.04.010 · Cited by 59 · see citing works
Mast Cells in Liver Fibrogenesis
Weiskirchen, R., Meurer, S. K., Liedtke, C., & Huber, M. (2019) · Cells · doi:10.3390/cells8111429 · Cited by 59 · see citing works
Expression of chymase‐positive cells in gastric cancer and its correlation with the angiogenesis
Kondo, K., Muramatsu, M., Okamoto, Y., Jin, D., Takai, S., Tanigawa, N., & Miyazaki, M. (2005) · Journal of Surgical Oncology · doi:10.1002/jso.20394 · Cited by 58 · see citing works
Anti-Inflammatory Effects of Eplerenone on Viral Myocarditis
Xiao, J., Shimada, M., Liu, W., Hu, D., & Matsumori, A. (2009) · European Journal of Heart Failure · doi:10.1093/eurjhf/hfp023 · Cited by 56 · see citing works
Mast Cell Chymase and Tryptase as Targets for Cardiovascular and Metabolic Diseases
He, A., & Shi, G. (2012) · Current Pharmaceutical Design · doi:10.2174/1381612811319060012 · Cited by 55 · see citing works
Edwards, S. T., Cruz, A. C., Donnelly, S., Dazin, P. F., Schulman, E. S., Jones, K. D., Wolters, P. J., Hoopes, C. W., Dolganov, G., & Fang, K. C. (2005) · The Journal of Pathology · doi:10.1002/path.1780 · Cited by 53 · see citing works
Self-citationIdentification of Matrix Metalloproteinases in Canine Cutaneous Mast Cell Tumors
Leibman, N. F., Lana, S. E., Hansen, R. A., Powers, B. E., Fettman, M. J., Withrow, S. J., & Ogilvie, G. K. (2000) · Journal of Veterinary Internal Medicine · doi:10.1111/j.1939-1676.2000.tb02280.x · Cited by 53 · see citing works
In vitro and in vivo Expression of InterstitialCollagenase/MMP‐1 by Human Mast Cells
Girolamo, N. D., & Wakefield, D. (2000) · Journal of Immunology Research · doi:10.1155/2000/82708 · Cited by 52 · see citing works
Ishida, K., Takai, S., Murano, M., Nishikawa, T., Inoue, T., Murano, N., Inoue, N., Jin, D., Umegaki, E., Higuchi, K., et al. (2007) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.107.131946 · Cited by 52 · see citing works
Kołaczkowska, E., Arnold, B., & Opdenakker, G. (2007) · Immunobiology · doi:10.1016/j.imbio.2007.07.005 · Cited by 50 · see citing works
Chymase-positive mast cells in small sized adenocarcinoma of the lung
Nagata, M., Shijubo, N., Walls, A. F., Ichimiya, S., Abe, S., & Sato, N. (2003) · Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin · doi:10.1007/s00428-003-0842-y · Cited by 46 · see citing works
Zheng, J., Wei, C., Hase, N., Shi, K., Killingsworth, C. R., Litovsky, S. H., Powell, P. C., Kobayashi, T., Ferrario, C. M., Rab, A., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0094732 · Cited by 45 · see citing works
Hovdenak, N., Wang, J., Sung, C., Kelly, T. J., Fajardo, L. F., & Hauer‐Jensen, M. (2002) · International Journal of Radiation Oncology*Biology*Physics · doi:10.1016/s0360-3016(02)02808-0 · Cited by 45 · see citing works
Carl‐McGrath, S., Gräntzdörffer, I., Lendeckel, U., Ebert, M. P., & Röcken, C. (2009) · Pathology · doi:10.1080/00313020902885037 · Cited by 44 · see citing works
Bałuk, P., Raymond, W. W., Ator, E., Coussens, L. M., McDonald, D. M., & Caughey, G. H. (2004) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00404.2003 · Cited by 36 · see citing works
Self-citationTransgenic study of the function of chymase in heart remodeling
Chen, L., Li, P., He, Q., Jiang, L., Cui, C.-J., Xu, L., & Liu, L. (2002) · Journal of Hypertension · doi:10.1097/00004872-200210000-00025 · Cited by 35 · see citing works
Mellon, M. B., Frank, B. T., & Fang, K. C. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.168.1.290 · Cited by 33 · see citing works
Self-citationProteases in Pemphigoid Diseases
Hiroyasu, S., Turner, C. T., Richardson, K. C., & Granville, D. J. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.01454 · Cited by 32 · see citing works
Microscopic localization of active gelatinases in equine osteochondritis dissecans (OCD) cartilage
Al-Hizab, F. A., Clegg, P., Thompson, C. C., & Carter, S. D. (2002) · Osteoarthritis and Cartilage · doi:10.1053/joca.2002.0811 · Cited by 32 · see citing works
Takai, S., Jin, D., Chen, H., Li, W., Yamamoto, H., Yamanishi, K., Miyazaki, M., Higashino, H., Yamanishi, H., & Okamura, H. (2014) · Journal of Hypertension · doi:10.1097/hjh.0000000000000231 · Cited by 31 · see citing works
Freitas, V. S., Santos, P. P. D. A., Freitas, R. D. A., Pinto, L. P., & de Souza, L. B. (2011) · Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology · doi:10.1016/j.tripleo.2011.02.032 · Cited by 31 · see citing works
Kurusu, A., Suzuki, Y., Horikoshi, S., Shirato, I., & Tomino, Y. (2001) · The Nephron journals/Nephron journals · doi:10.1159/000046109 · Cited by 31 · see citing works
Increased fibroblast chymase production mediates procollagen autophagic digestion in volume overload
Fu, L., Wei, C., Powell, P. C., Bradley, W. E., Ahmad, S., Ferrario, C. M., Collawn, J. F., & Dell’Italia, L. J. (2016) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2016.01.019 · Cited by 29 · see citing works
Generation and characterization of bone marrow-derived cultured canine mast cells
Lin, T., Rush, L. J., & London, C. A. (2006) · Veterinary Immunology and Immunopathology · doi:10.1016/j.vetimm.2006.03.024 · Cited by 29 · see citing works
Kakimoto, K., Takai, S., Murano, M., Ishida, K., Yoda, Y., Inoue, T., Jin, D., Umegaki, E., & Higuchi, K. (2009) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.109.162933 · Cited by 28 · see citing works
Mina, Y., Rinkevich-Shop, S., Konen, E., Goitein, O., Kushnir, T., Epstein, F. H., Feinberg, M. S., Leor, J., & Landa‐Rouben, N. (2012) · Journal of Cardiovascular Pharmacology and Therapeutics · doi:10.1177/1074248412458975 · Cited by 28 · see citing works
Chiba, N., Shimada, K., Chen, S., Jones, H. D., Alsabeh, R., Slepenkin, A. V., Peterson, E. M., Crother, T. R., & Arditi, M. (2015) · The Journal of Immunology · doi:10.4049/jimmunol.1402685 · Cited by 27 · see citing works
The physiological response of protease inhibition in dystrophic muscle
Hollinger, K., & Selsby, J. T. (2013) · Acta Physiologica · doi:10.1111/apha.12114 · Cited by 26 · see citing works
A functional comparison of canine and murine bone marrow derived cultured mast cells
Lin, T., & London, C. A. (2006) · Veterinary Immunology and Immunopathology · doi:10.1016/j.vetimm.2006.09.001 · Cited by 23 · see citing works
Mast Cell Chymase and Kidney Disease
Vibhushan, S., Bratti, M., Montero-Hernández, J. E., El‐Ghoneimi, A., Benhamou, M., Charles, N., Daugas, É., & Blank, U. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms22010302 · Cited by 21 · see citing works
Lemnalol attenuates mast cell activation and osteoclast activity in a gouty arthritis model
Lee, H.-P., Lin, Y., Duh, C., Huang, S., Wang, H., Wu, S.-F., Lin, S., Jean, Y., & Wen, Z. (2014) · Journal of Pharmacy and Pharmacology · doi:10.1111/jphp.12331 · Cited by 19 · see citing works
Tanaka, A., & Matsuda, H. (2004) · Cellular Immunology · doi:10.1016/j.cellimm.2004.03.009 · Cited by 19 · see citing works
Heterotypic adhesion-induced mast cell activation: biologic relevance in the inflammatory context
Mekori, Y. A., & Baram, D. (2002) · Molecular Immunology · doi:10.1016/s0161-5890(02)00089-5 · Cited by 19 · see citing works
Metalloproteinases in juvenile angiofibroma—a collagen rich tumor
Duerr, S., Wendler, O., Aigner, T. G., Karosi, S., & Schick, B. (2007) · Human Pathology · doi:10.1016/j.humpath.2007.06.015 · Cited by 18 · see citing works
Ebihara, N., Takai, S., Miyazaki, M., & Murakami, A. (2005) · Current Eye Research · doi:10.1080/02713680590959367 · Cited by 17 · see citing works
Connective Tissue-Type Mast Cell Leukemia in a Dog.
Hikasa, Y., MORITA, T., FUTAOKA, Y., Sato, K., Shimada, A., KAGOTA, K., & Matsuda, H. (2000) · Journal of Veterinary Medical Science · doi:10.1292/jvms.62.187 · Cited by 17 · see citing works
Tomimori, Y., Manno, A., Tanaka, T., Futamura-Takahashi, J., Muto, T., & Nagahira, K. (2019) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2019.05.032 · Cited by 17 · see citing works
A Pulmonary Perspective on GASPIDs: Granule-Associated Serine Peptidases of Immune Defense
Caughey, G. H. (2006) · Current Respiratory Medicine Reviews · doi:10.2174/157339806778019024 · Cited by 16 · see citing works
Self-citationMast Cells and Basophils: Trojan Horses of Conventional Lin- Stem/Progenitor Cell Isolates
Heneberg, P. (2011) · Current Pharmaceutical Design · doi:10.2174/138161211798357881 · Cited by 15 · see citing works
A relation between TGF-β and mast cell tryptase in experimental emphysema models
Mortaz, E., Givi, M. E., Silva, C. A. D., Folkerts, G., & Redegeld, F. A. (2012) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2012.03.006 · Cited by 15 · see citing works
Docampo, M. J., Cabrera, J. S., Rabanal, R. M., & Bassols, A. (2011) · American Journal of Veterinary Research · doi:10.2460/ajvr.72.8.1087 · Cited by 13 · see citing works
Characterization of gelatinase produced by Antarctic Mrakia sp.
Yuivar, Y., Alcaı́no, J., Cifuentes, V., & Baeza, M. (2019) · Journal of Basic Microbiology · doi:10.1002/jobm.201900126 · Cited by 13 · see citing works
Kanako, M. I. Y. A., MISUMI, K., Miyoshi, N., Koh, A. R. A. I., FUJIKI, M., Kubota, C., & SAKAMOTO, H. (2005) · Journal of Veterinary Medical Science · doi:10.1292/jvms.67.769 · Cited by 13 · see citing works
Deranged aortic intima‐media thickness, plasma triglycerides and granulopoiesis in Sl/Sld mice
Dileepan, K. N., Johnston, T. P., Li, Y., Tawfik, O. W., & Stechschulte, D. J. (2004) · Mediators of Inflammation · doi:10.1080/09629350400008794 · Cited by 13 · see citing works
Imai, Y., Takai, S., Jin, D., Komeda, K., Tashiro, K., Li, Z., Otsuki, Y., Okamura, H., Hayashi, M., & Uchiyama, K. (2014) · Pharmacology · doi:10.1159/000357684 · Cited by 13 · see citing works
Wang, Y., Liu, C.-L., Fang, W., Zhang, X., Yang, C., Li, J., Liu, J., Sukhova, G. K., Gurish, M. F., Libby, P., et al. (2019) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2019.01.011 · Cited by 9 · see citing works
- Authors
- Fang, K. C., Raymond, W. W., Lazarus, S. C., & Caughey, G. H.
- Affiliations
- University of California, San Francisco (US)
- Venue
- Journal of Clinical Investigation
- Published
- 1996-04-01
- Volume
- 97
- Issue
- 7
- Pages
- 1589-1596
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC507221
- OpenAlex
- W1967763193
- Cited by
- 125
- References
- 40
- Citations by year
- 2025: 2 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 3 · 2019: 8 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 4 · 2014: 7 · 2013: 2 · 2012: 5
- FWCI
- 1.79
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- American Lung Association
- National Institutes of Health (HL24136)
- National Heart, Lung, and Blood Institute (F32HL009133, HL 24136, P01HL024136, HL 07185, HL09133)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Veterinary Oncology Research
- Keywords
- mastocytoma, chymase, MMP-9, serine protease, mast cell degranulation, tumor invasion, connective tissue remodeling, tryptase
- MeSH terms
- Amino Acid Sequence, Animals, Dogs, Enzyme Activation, Enzyme Inhibitors, Humans, Mast Cells, Molecular Sequence Data, Molecular Weight, Rabbits, Mast-Cell Sarcoma, Serine Endopeptidases, Tumor Cells, Cultured, Sequence Homology, Amino Acid, Gelatinases, Mice, Chymases
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