The DOI matches its Crossref record; 74 of the 74 references it cites were found in a registry or an index.
WNT1-inducible signaling protein–1 mediates pulmonary fibrosis in mice and is upregulated in humans with idiopathic pulmonary fibrosis
Open accessAbstract
Idiopathic pulmonary fibrosis (IPF) is characterized by distorted lung architecture and loss of respiratory function. Enhanced (myo)fibroblast activation, ECM deposition, and alveolar epithelial type II (ATII) cell dysfunction contribute to IPF pathogenesis. However, the molecular pathways linking ATII cell dysfunction with the development of fibrosis are poorly understood. Here, we demonstrate, in a mouse model of pulmonary fibrosis, increased proliferation and altered expression of components of the WNT/beta-catenin signaling pathway in ATII cells. Further analysis revealed that expression of WNT1-inducible signaling protein-1 (WISP1), which is encoded by a WNT target gene, was increased in ATII cells in both a mouse model of pulmonary fibrosis and patients with IPF. Treatment of mouse primary ATII cells with recombinant WISP1 led to increased proliferation and epithelial-mesenchymal transition (EMT), while treatment of mouse and human lung fibroblasts with recombinant WISP1 enhanced deposition of ECM components. In the mouse model of pulmonary fibrosis, neutralizing mAbs specific for WISP1 reduced the expression of genes characteristic of fibrosis and reversed the expression of genes associated with EMT. More importantly, these changes in gene expression were associated with marked attenuation of lung fibrosis, including decreased collagen deposition and improved lung function and survival. Our study thus identifies WISP1 as a key regulator of ATII cell hyperplasia and plasticity as well as a potential therapeutic target for attenuation of pulmonary fibrosis.
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“Moreover, transcript level as well as secretion of the ATII cell-derived fibrotic mediator Wnt-inducible signalling protein (Wisp) 1 [26] were significantly reduced upon senolytic treatment (online supplementary figure S5C and figure 5d; DMSO 2.10±0.91-fold over control versus DQ 0.71±0.26-fold over control).”
From Senolytic drugs target alveolar epithelial cell function and attenuate experimental lung fibrosis ex vivo (2017) · cites this paper as [26]
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Cites
The Wnt Signaling Pathway in Development and Disease
Logan, C. Y., & Nusse, R. (2004) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.20.010403.113126 · Cited by 5,325
(2002) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.165.2.ats01 · Cited by 4,196
Epithelial-mesenchymal transition and its implications for fibrosis
Kalluri, R., & Neilson, E. G. (2003) · Journal of Clinical Investigation · doi:10.1172/jci20530 · Cited by 2,715
More powerful procedures for multiple significance testing
Hochberg, Y., & Benjamini, Y. (1990) · Statistics in Medicine · doi:10.1002/sim.4780090710 · Cited by 2,715
Selman, M., King, T. E., & Pardo, A. (2001) · Annals of Internal Medicine · doi:10.7326/0003-4819-134-2-200101160-00015 · Cited by 1,807
WNT and β-catenin signalling: diseases and therapies
Moon, R. T., Kohn, A. D., Ferrari, G. V. D., & Kaykas, A. (2004) · Nature Reviews Genetics · doi:10.1038/nrg1427 · Cited by 1,763
Idiopathic Pulmonary Fibrosis: Clinical Relevance of Pathologic Classification
KATZENSTEIN, A.-L. A., & MYERS, J. L. (1998) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.157.4.9707039 · Cited by 1,261
Kim, K. K., Kugler, M. C., Wolters, P. J., Robillard, L., Galvez, M. G., Brumwell, A. N., Sheppard, D., & Chapman, H. A. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0605669103 · Cited by 1,257
Circulating fibrocytes traffic to the lungs in response to CXCL12 and mediate fibrosis
Phillips, R. J., Burdick, M. D., Hong, K. M., Lutz, M., Murray, L. A., Xue, Y., Belperio, J. A., Keane, M. P., & Strieter, R. M. (2004) · Journal of Clinical Investigation · doi:10.1172/jci20997 · Cited by 1,071
TGF-β-induced EMT: mechanisms and implications for fibrotic lung disease
Willis, B. C., & Borok, Z. (2007) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00163.2007 · Cited by 1,064
Flagged first, then by citations
Research works printed but not linked: 7
- Gharaee-Kermani, 2005, Methods Mol. Med., 117, 251
- Phan, 2003, Am. J. Respir. Cell Mol. Biol., 29, S87
- Kalluri, 2003, J. Clin. Invest., 112, 1776, 10.1172/JCI200320530 · DOI
- Uhal, 1998, Am. J. Physiol., 275, L1192
- Adamson, 1988, Am. J. Pathol., 130, 377
- Fukuda, 1985, Am. J. Pathol., 118, 452
- Phillips, 2004, J. Clin. Invest., 114, 438, 10.1172/JCI200420997 · DOI
Cited by
Sorted by citations
Items 501–553 of 553
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Zhang, L. (2016) · Journal of Anesthesia & Critical Care Open Access · doi:10.15406/jaccoa.2016.04.00133 · Cited by 1
Analysis of Epithelial Injury and Repair
Mutze, K., & Königshoff, M. (2016) · Respiratory medicine · doi:10.1007/978-3-319-46527-2_6 · Cited by 1
Self-citationRegenerative Potential of Mesenchymal Stem Cells: Therapeutic Applications in Lung Disorders
Sharma, K., Husain, S. Y., Das, P., Hussain, M., & Syed, M. A. (2016) · Stem cells in clinical applications · doi:10.1007/978-3-319-46693-4_6 · Cited by 1
Drug Discovery in Tissue Fibrosis
Pinto, S., Hoek, M., Huang, Y., Costet, P., Ma, L., & Imbriglio, J. E. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-409547-2.12432-1 · Cited by 1
Loeh, B., Korfei, M., Mahavadi, P., Wasnick, R. M., von der Beck, D., & Günther, A. (2016) · European Respiratory Society eBooks · doi:10.1183/2312508x.10004815 · Cited by 1
Self-citationEpithelial biology in idiopathic pulmonary fibrosis
Selman, M., & Pardo, A. (2015) · doi:10.2217/fmeb2013.13.307 · Cited by 1
Molecular Genetics of Dupuytren´s Disease
Becker, K. (2012) · Kölner Universitäts PublikationsServer (Universität zu Köln)
Expressions of Wingless and Int1 (Wnt)-induced secreted protein 1 in paraquat-poisoned patients
Liu, J., Guo, Y.-J., Song, Y.-Z., Song, X.-L., & Lin, D.-J. (2017) · Tropical Journal of Pharmaceutical Research · doi:10.4314/tjpr.v16i1.30
Molecular Mechanisms of Scleroderma and Fibrosis
(2017) · International journal of research studies in biosciences · doi:10.20431/2349-0365.0501007
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Fernandez, I. E. (2018) · Elektronische Hochschulschriften der LMU München (Ludwig-Maximilians-Universität München) · doi:10.5282/edoc.23287
Urinary Proteome Biomarkers for Early Detection of Respiratory Diseases
Wu, J., & Huang, H. (2019) · Springer eBooks · doi:10.1007/978-981-13-9109-5_13
ATS 2019 et BPCO: une nouvelle ère pour des thérapeutiques innovantes ?
Zysman, M. (2019) · Revue des Maladies Respiratoires Actualités · doi:10.1016/s1877-1203(19)30034-5
Park, S., Jung, J., & Joo, J. W. J. (2020) · Joint International Conference on Soft Computing and Intelligent Systems and International Symposium on Advanced Intelligent Systems (SCIS&ISIS) · doi:10.1109/scisisis50064.2020.9322727
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(2010) · Current Opinion in Rheumatology · doi:10.1097/bor.0b013e3283404094
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Cohen, M., & Wolters, P. J. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-801238-3.11657-5
Sandbo, N. K. (2018) · Respiratory medicine · doi:10.1007/978-3-319-99975-3_7
Sun, C., Ma, H., Yin, J., Zhang, H., & Liu, H. (2020) · doi:10.22541/au.160495490.00159330/v1
Martel‐Pelletier, J., & Kapoor, M. (2012) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.715697815.791102869
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Gaudreau, P.-O., Cousineau, I., & Stagg, J. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2744-0_2
Pulmonary Fibrosis: One Manifestation, Various Diseases
Ruaro, B., Matucci‐Cerinic, M., Baratella, E., Confalonieri, M., Salton, F., & Hughes, M. (2022) · Frontiers research topics · doi:10.3389/978-2-83250-654-7
Supplementary Methods from Canonical Wnt Signaling Is Required for Pancreatic Carcinogenesis
Zhang, Y., Morris, J. P., Yan, W., Schofield, H. K., Gurney, A. L., Simeone, D. M., Millar, S. E., Hoey, T. C., Hebrok, M., & di Magliano, M. P. (2023) · doi:10.1158/0008-5472.22394940
Jaggi, D., Aggarwal, K., & Goyal, L. (2023) · STEMedicine · doi:10.37175/stemedicine.v4i4.185
Xu, H., Hong, J., Liu, D., Wu, X., Yang, X., & Yu, Z. (2023) · Research Square · doi:10.21203/rs.3.rs-3753532/v1
LncRNA-mediated gene regulation in cardiopulmonary development, homeostasis and disease
Rogala, S. (2024) · Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt) · doi:10.21248/gups.85674
Cheng, W.-C., Chen, P. Y., Zhang, X., Chang, Y., Tan, K., & Lin, T. C. C. (2024) · Immunopharmacology and Immunotoxicology · doi:10.1080/08923973.2024.2371150
Wang, T., Wang, W., Xu, C., Tian, X., & Zhang, D. (2024) · BMC Genomics · doi:10.1186/s12864-024-11165-6
Zhang, Y., Wang, Y., & Zhao, F. (2025) · Molecular Neurobiology · doi:10.1007/s12035-025-04743-6 · Cited by 1
Progress of Urinary Proteome Research in Respiratory Diseases
Wu, J., Wang, S., & Qin, X. (2025) · Springer eBooks · doi:10.1007/978-981-96-7886-0_16
The Role of IL-33 and RUNX2 in the Pathogenesis of Idiopathic Pulmonary FibrosisPreprint
Liu, S., Wang, L., Yue, Y., Zhang, Z., Li, F., Wu, D., Zhang, H., & Wu, Q. (2025) · Research Square · doi:10.21203/rs.3.rs-7626166/v1
Rebeca, F.-R., Garcia-Alaman, A., Fontcuberta-PiSunyer, M., Perea-Atienzar, M., Angulo-Capel, J., Hadley, C. K., Lin, Z., Cohen, P., Vidal, J., & Gasa, R. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.01.15.699691
Alzate‐Correa, D., Stranan, J. P., Rincon‐Benavides, M. A., Mazo, N. A., Narváez-Pérez, L. F., Jaramillo-Garrido, S., Nguyen, T., Patel, R., Lawrence, W. R., Diaz‐Starokozheva, L., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.01.22.700900
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Parkins, S., Anthony, S., Goldsworthy, T. K., Nigam, A., Schehl, N. C., Jaggers, R. M., Kasprovic, D. A., Green, L. C., Kanisicak, O., & Tranter, M. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.02.17.706476
Ji, T., Lv, Y., & Gu, J. (2026) · International Journal of COPD · doi:10.2147/copd.s561510
Rôle de la voie hedgehog dans la fibrose pulmonaire idiopathique
Moshai, E. F. (2013) · theses.fr (ABES) · doi:10.70675/b550340ez33a1z43b7zb4eez8c2cfde874df
Henrot, P. (2018) · theses.fr (ABES) · doi:10.70675/a419e36azc56fz4becz9537z0cc77ceae5ba
Suppressing p16INK4a promotes lung regeneration through a fibroblast lipogenic switch
Zysman, M. (2019) · theses.fr (ABES) · doi:10.70675/c507eaa0z188ez4332z952az2c54118917d6
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Hu, Q., Onwuka, U., Cárdenes, N., Packwood, M., Huang, E. L., Shi, J., Melo-Narváez, M. C., Dutta, P., Zhou, Z., Beaulieu, D., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.05.28.728065
Self-citationMocci, S., Littera, R., Deidda, S., Cannas, F., Cocco, C., Lorrai, M., Mereu, C., Murgia, M., Nutile, G., Lai, F., et al. (2026) · BMJ Open Respiratory Research · doi:10.1136/bmjresp-2025-003625
Ren, M., Guihua, S. O. N. G., Xu, Y., Yu, S., Zhang, Y., Sun, M., & Zhang, B. (2026) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2026.1914253
Singh, P., Chakraborty, K., Bansal, A., Agrawal, A., & Kukreti, R. (2026) · Molecular Biology Reports · doi:10.1007/s11033-026-12709-7
Cellular Senescence and Premature Aging in Lung Diseases
Aoshiba, K., Tsuji, T., Yamaguchi, K., Itoh, M., & Nakamura, H. (2013) · Elsevier eBooks · doi:10.1016/b978-0-12-397803-5.00017-4
Metadata
- Authors
- Königshoff, M., Kramer, M., Balsara, N., Wilhelm, J., Amarie, O. V., Jahn, A., Rose, F., Fink, L., Seeger, W., Schaefer, L., Günther, A., & Eickelberg, O.
- Affiliations
- Justus-Liebig-Universität Gießen (DE)
- Philipps University of Marburg (DE)
- Goethe University Frankfurt (DE)
- Helmholtz Munich (DE)
- Corresponding author
- Eickelberg, Oliver
- ORCID
- Venue
- Journal of Clinical Investigation
- Published
- 2009-03-16
- Volume
- 119
- Issue
- 4
- Pages
- 772-87
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC2662540
- OpenAlex
- W2157101382
- Cited by
- 558
- References
- 72
- Citations by year
- 2026: 11 · 2025: 19 · 2024: 29 · 2023: 22 · 2022: 25 · 2021: 27 · 2020: 36 · 2019: 26 · 2018: 44 · 2017: 41 · 2016: 53 · 2015: 42 · 2014: 33 · 2013: 42 · 2012: 40
- FWCI
- 30.40
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Site cant be reached (submittedVersion)
- Funders
- Alexander von Humboldt-Stiftung
- Deutsche Forschungsgemeinschaft
- Helmholtz Zentrum München
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Pulmonary Hypertension Research and Treatments
- Health Sciences › Medicine › Surgery › Transplantation: Methods and Outcomes
- Keywords
- WISP1, idiopathic pulmonary fibrosis, alveolar type II cells, Wnt/β-catenin signaling, epithelial-mesenchymal transition, extracellular matrix deposition, myofibroblast activation, collagen deposition, pulmonary fibrosis, therapeutic target, neutralizing antibodies
- MeSH terms
- Adult, Animals, Bleomycin, Disease Models, Animal, Epithelial Cells, Female, Humans, Male, Mice, Inbred C57BL, Mice, Transgenic, Middle Aged, Models, Biological, Proto-Oncogene Proteins, Pulmonary Alveoli, Pulmonary Fibrosis, Recombinant Proteins, Oncogene Proteins, Up-Regulation, Intracellular Signaling Peptides and Proteins, Cell Proliferation, Wnt Proteins, beta Catenin, Mice, Idiopathic Pulmonary Fibrosis, CCN Intercellular Signaling Proteins
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