The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Engineering the gut microbiota to treat hyperammonemia
Open accessAbstract
Increasing evidence indicates that the gut microbiota can be altered to ameliorate or prevent disease states, and engineering the gut microbiota to therapeutically modulate host metabolism is an emerging goal of microbiome research. In the intestine, bacterial urease converts host-derived urea to ammonia and carbon dioxide, contributing to hyperammonemia-associated neurotoxicity and encephalopathy in patients with liver disease. Here, we engineered murine gut microbiota to reduce urease activity. Animals were depleted of their preexisting gut microbiota and then inoculated with altered Schaedler flora (ASF), a defined consortium of 8 bacteria with minimal urease gene content. This protocol resulted in establishment of a persistent new community that promoted a long-term reduction in fecal urease activity and ammonia production. Moreover, in a murine model of hepatic injury, ASF transplantation was associated with decreased morbidity and mortality. These results provide proof of concept that inoculation of a prepared host with a defined gut microbiota can lead to durable metabolic changes with therapeutic utility.
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Cites
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Cited by
Current Understanding of Dysbiosis in Disease in Human and Animal Models
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Mimee, M., Citorik, R. J., & Lu, T. K. (2016) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2016.04.032 · Cited by 317
Metadata
- Authors
- Shen, T.-C. D., Albenberg, L., Bittinger, K., Chehoud, C., Chen, Y.-Y., Judge, C. A., Chau, L., Ni, J., Sheng, M., Lin, A., Wilkins, B. J., Buza, E. L., Lewis, J. D., Daikhin, Y., Nissim, I., Yudkoff, M., Bushman, F. D., & Wu, G. D.
- Venue
- Journal of Clinical Investigation
- Published
- 2015-06-22
- Volume
- 125
- Issue
- 7
- Pages
- 2841-2850
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2112931540
- Cited by
- 180
- References
- 8
- FWCI
- 6.82
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Shen, T.-C. D., Albenberg, L., Bittinger, K., Chehoud, C., Chen, Y.-Y., Judge, C. A., Chau, L., Ni, J., Sheng, M., Lin, A., Wilkins, B. J., Buza, E. L., Lewis, J. D., Daikhin, Y., Nissim, I., Yudkoff, M., Bushman, F. D., & Wu, G. D. (2015). Engineering the gut microbiota to treat hyperammonemia. Journal of Clinical Investigation. https://doi.org/10.1172/jci79214
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