Immunocytochemical Localization of Type 5 17 β-Hydroxysteroid Dehydrogenase in Human Reproductive Tissues
Abstract
17beta-hydroxysteroid dehydrogenase (17beta-HSD) controls the last step in the formation of all androgens and all estrogens. At least six 17beta-HSD isoenzymes have been identified. The recently cloned Type 5 17beta-HSD transforms 4-dione into testosterone. To gain a better understanding of the role of this enzyme in reproductive tissues, we immunocytochemically localized the enzyme in human male and female reproductive organs. In the ovary of adult premenopausal women (25-40 years of age), immunostaining was found in corpus luteum cells. In the uterus, staining was detected only in the epithelial cells of the endometrium. Immunolabeling was also detected in the mammary gland, a positive reaction being detected in epithelial cells of acini and intralobular ducts as well as in the surrounding stromal cells. In the testis, strong staining was seen in the Leydig cells, and a weak but specific reaction was occasionally detected in Sertoli and germ cells. In the prostate, specific labeling was observed in alveoli and stromal fibroblasts. In alveoli, all the basal cells were generally labeled, whereas the luminal cells exhibited variations in immunoreactivity. In all the reproductive organs examined, specific staining was routinely detected in the walls of blood vessels, including the endothelial cells. These results indicate a cell-specific localization of Type 5 17beta-HSD in the different human reproductive organs, thus suggesting new mechanisms of local androgen and estrogen formation that may play an important physiological role.
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AKR1C3 expression has been reported in Leydig cells and may also contribute to testosterone production.
“AKR1C3 expression has been reported in Leydig cells and may also contribute to testosterone production [49,50].”
From Human steroid biosynthesis, metabolism and excretion are differentially reflected by serum and urine steroid metabolomes: a comprehensive review (2019) · cites this paper as [49]
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- Authors
- Pelletier, G., Luu-The, V., Tětu, B., & Labrie, F.
- Affiliations
- Centre hospitalier de l'Université Laval (CA)
- Laval University, Pathology Department, Hôtel-Dieu de Québec, Québec, Canada
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1999-06
- Volume
- 47
- Issue
- 6
- Pages
- 731-737
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2164373167
- Cited by
- 88
- References
- 37
- Citations by year
- 2022: 3 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 4
- FWCI
- 2.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Histochemistry & Cytochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sexual Differentiation and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Keywords
- 17β-hydroxysteroid dehydrogenase type 5, enzyme localization, androgen production, estrogen synthesis, corpus luteum, endometrium, mammary gland, Leydig cells, prostate
- MeSH terms
- Breast, Female, Genitalia, Female, Genitalia, Male, Humans, Immunohistochemistry, Male, Ovary, Prostate, Testis, Uterus, 17-Hydroxysteroid Dehydrogenases
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