Bio-printing Cell-Laden Matrigel-Agarose Constructs
Open accessAbstract
3D printing of biological architectures that mimic the structural and functional features of in vivo tissues is of great interest in tissue engineering and the development of transplantable organ constructs. Printable bio-inks that are compatible with cellular activities play critical roles in the process of 3D bio-printing. Although a variety of hydrogels have been used as bio-inks for 3D bio-printing, they inherit poor mechanical properties and/or the lack of essential protein components that compromise their performance. Here, a hybrid Matrigel-agarose hydrogel system has been demonstrated that possesses both desired rheological properties for bio-printing and biocompatibility for long-term (11 days) cell culture. The agarose component in the hybrid hydrogel system enables the maintenance of 3D-printed structures, whereas Matrigel provides essential microenvironments for cell growth. When human intestinal epithelial HCT116 cells are encapsulated in the printed Matrigel-agarose constructs, high cell viability and proper cell spreading morphology are observed. Given that Matrigel is used extensively for 3D cell culturing, the developed 3D-printable Matrigel-agarose system will open a new way to construct Matrigel-based 3D constructs for cell culture and tissue engineering.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The polymerization temperature for Matrigel of 22-37°C, mediated by entactin interaction with laminins and collagen IV, limits its bioprintability in the absence of temperature-controlled environments or as a hybrid bioink.
“Further, the polymerization temperature for Matrigel of 22–37°C, mediated by entactin interaction with laminins and collagen IV, also limits its bioprintability in the absence of temperature-controlled environments or as a hybrid bioink (Fan et al., 2016).”
From Three-dimensional modelling of ovarian cancer: from cell lines to organoids for discovery and personalized medicine (2022) · cites this paper as Fan et al., 2016
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Hydrogels as extracellular matrix mimics for 3D cell culture
Tibbitt, M. W., & Anseth, K. S. (2009) · Biotechnology and Bioengineering · doi:10.1002/bit.22361 · Cited by 2,727 · see citing works
Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
Miller, J. S., Stevens, K. R., Yang, M. T., Baker, B. M., Nguyen, D.-H. T., Cohen, D. M., Toro, E., Chen, A. A., Galie, P. A., Yu, X., et al. (2012) · Nature Materials · doi:10.1038/nmat3357 · Cited by 1,917 · see citing works
Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate
Huebsch, N., Arany, P. R., Mao, A. S., Shvartsman, D. E., Ali, O. A., Bencherif, S. A., Rivera‐Feliciano, J., & Mooney, D. J. (2010) · Nature Materials · doi:10.1038/nmat2732 · Cited by 1,572 · see citing works
Designing materials to direct stem-cell fate
Lütolf, M. P., Gilbert, P. M., & Blau, H. M. (2009) · Nature · doi:10.1038/nature08602 · Cited by 1,389 · see citing works
Scaffold-free vascular tissue engineering using bioprinting
Norotte, C., Marga, F., Niklason, L. E., & Forgács, G. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.06.034 · Cited by 1,342 · see citing works
Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications
Sato, T., & Clevers, H. (2013) · Science · doi:10.1126/science.1234852 · Cited by 1,170 · see citing works
The role of matrix stiffness in regulating cell behavior†
Wells, R. G. (2008) · Hepatology · doi:10.1002/hep.22193 · Cited by 1,055 · see citing works
3D Bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels
Duan, B., Hockaday, L. A., Kang, K. H., & Butcher, J. T. (2012) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.34420 · Cited by 1,005 · see citing works
3D Printing of Highly Stretchable and Tough Hydrogels into Complex, Cellularized Structures
Hong, S., Sycks, D. G., Chan, H. F., Lin, S., López, G. P., Guilak, F., Leong, K. W., & Zhao, X. (2015) · Advanced Materials · doi:10.1002/adma.201501099 · Cited by 875 · see citing works
A photolabile hydrogel for guided three-dimensional cell growth and migration
Luo, Y., & Shoichet, M. S. (2004) · Nature Materials · doi:10.1038/nmat1092 · Cited by 801 · see citing works
Awad, H. A., Wickham, M. Q., Leddy, H. A., Gimble, J. M., & Guilak, F. (2003) · Biomaterials · doi:10.1016/j.biomaterials.2003.10.045 · Cited by 794 · see citing works
Direct Human Cartilage Repair Using Three-Dimensional Bioprinting Technology
Cui, X., Breitenkamp, K., Finn, M. G., Lotz, M., & DʼLima, D. D. (2012) · Tissue Engineering Part A · doi:10.1089/ten.tea.2011.0543 · Cited by 688 · see citing works
Bioprinted Amniotic Fluid-Derived Stem Cells Accelerate Healing of Large Skin Wounds
Skardal, A., Mack, D. L., Kapetanovic, E., Atala, A., Jackson, J. D., Yoo, J. J., & Söker, S. (2012) · Stem Cells Translational Medicine · doi:10.5966/sctm.2012-0088 · Cited by 663 · see citing works
Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels
Bertassoni, L. E., Cardoso, J. C., Manoharan, V., Cristino, A. L., Bhise, N. S., Araujo, W. A., Zorlutuna, P., Vrana, N. E., Ghaemmaghami, A. M., Dokmeci, M. R., et al. (2014) · Biofabrication · doi:10.1088/1758-5082/6/2/024105 · Cited by 642 · see citing works
Michael, S., Sorg, H., Peck, C., Koch, L., Deiwick, A., Chichkov, B. N., Vogt, P. M., & Reimers, K. (2013) · PLoS ONE · doi:10.1371/journal.pone.0057741 · Cited by 625 · see citing works
Gelain, F., Bottai, D., Vescovi, A., & Zhang, S. (2006) · PLoS ONE · doi:10.1371/journal.pone.0000119 · Cited by 612 · see citing works
Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures
Balgude, A. (2001) · Biomaterials · doi:10.1016/s0142-9612(00)00350-1 · Cited by 520 · see citing works
Skardal, A., Zhang, J., & Prestwich, G. D. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.04.045 · Cited by 465 · see citing works
The effect of matrix stiffness on mesenchymal stem cell differentiation in a 3D thixotropic gel
Pek, Y. S., Wan, A. C. A., & Ying, J. Y. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.09.057 · Cited by 384 · see citing works
Elucidating the Role of Matrix Stiffness in 3D Cell Migration and Remodeling
Ehrbar, M., Sala, A., Lienemann, P. S., Ranga, A., Mosiewicz, K. A., Bittermann, A. G., Rizzi, S. C., Weber, F. E., & Lütolf, M. P. (2011) · Biophysical Journal · doi:10.1016/j.bpj.2010.11.082 · Cited by 361 · see citing works
Xu, F., Celli, J., Rizvi, I., Moon, S., Hasan, T., & Demirci, U. (2011) · Biotechnology Journal · doi:10.1002/biot.201000340 · Cited by 337 · see citing works
Bioprinting Endothelial Cells With Alginate for 3D Tissue Constructs
Khalil, S., & Sun, W. (2009) · Journal of Biomechanical Engineering · doi:10.1115/1.3128729 · Cited by 337 · see citing works
3D spheroid culture of hESC/hiPSC-derived hepatocyte-like cells for drug toxicity testing
Takayama, K., Kawabata, K., Nagamoto, Y., Kishimoto, K., Tashiro, K., Sakurai, F., Tachibana, M., Kanda, K., Hayakawa, T., Furue, M. K., et al. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.11.029 · Cited by 289 · see citing works
Campos, D. F. D., Blaeser, A., Weber, M. H., Jäkel, J., Neuß, S., Jahnen‐Dechent, W., & Fischer, H. (2012) · Biofabrication · doi:10.1088/1758-5082/5/1/015003 · Cited by 224 · see citing works
Topology Evolution and Gelation Mechanism of Agarose Gel
Xiong, J.-Y., Narayanan, J., Liu, X., et al. (2005) · The Journal of Physical Chemistry B · doi:10.1021/jp044473u · Cited by 210 · see citing works
Zhang, Y., Wu, S., Xia, Y., & Sun, J. (2014) · Physiological Reports · doi:10.14814/phy2.12147 · Cited by 203 · see citing works
Self-citation3D printing facilitated scaffold-free tissue unit fabrication
Tan, Y. J., Richards, D., Trusk, T. C., Visconti, R. P., Yost, M. J., Kindy, M. S., Drake, C. J., Argraves, W. S., Markwald, R. R., & Mei, Y. (2014) · Biofabrication · doi:10.1088/1758-5082/6/2/024111 · Cited by 151 · see citing works
O’Connor, S. M., Stenger, D. A., Shaffer, K. M., et al. (2001) · Neuroscience Letters · doi:10.1016/s0304-3940(01)01769-4 · Cited by 136 · see citing works
Distinct Tissue Formation by Heterogeneous Printing of Osteo- and Endothelial Progenitor Cells
Fedorovich, N. E., Wijnberg, H. M., Dhert, W. J. A., & Alblas, J. (2011) · Tissue Engineering Part A · doi:10.1089/ten.tea.2011.0019 · Cited by 126 · see citing works
Lu, Y., Song, W., An, D., Kim, B. J., Schwartz, R. E., Wu, M., & Ma, M. (2014) · Journal of Materials Chemistry B · doi:10.1039/c4tb01735h · Cited by 108 · see citing works
Lang, S., SHARRARD, R. M., Stark, M., Villette, J. M., & Maitland, N. J. (2001) · British Journal of Cancer · doi:10.1054/bjoc.2001.1967 · Cited by 106 · see citing works
c-myc GENE ABNORMALITIES IN MUCOSA-ASSOCIATED LYMPHOID TISSUE (MALT) LYMPHOMAS
Peng, H., Diss, T. C., Isaacson, P. G., & Pan, L. (1997) · The Journal of Pathology · doi:10.1002/(sici)1096-9896(199704)181:4 · Cited by 43 · see citing works
Monitoring nutrient transport in tissue-engineered grafts
Liu, J., Hilderink, J., Groothuis, T. A., Otto, C., van Blitterswijk, C., & de Boer, J. (2013) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.1654 · Cited by 39 · see citing works
Optimization of 3-D organotypic primary colonic cultures for organ-on-chip applications
Ahmad, A. A., Wang, Y., Gracz, A. D., Sims, C. E., Magness, S. T., & Allbritton, N. L. (2014) · Journal of Biological Engineering · doi:10.1186/1754-1611-8-9 · Cited by 34 · see citing works
Sequential assembly of 3D perfusable microfluidic hydrogels
He, J., Zhu, L., Liu, Y., Li, D., & Jin, Z. (2014) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-014-5270-9 · Cited by 22 · see citing works
Leaf-inspired artificial microvascular networks (LIAMN) for three-dimensional cell culture
Fan, R., Sun, Y.-H., & Wan, J. (2015) · RSC Advances · doi:10.1039/c5ra20265e · Cited by 15 · see citing works
Self-citationMEASURING ELASTIC PROPERTIES OF THIN BIOLOGICAL FILMS USING CAPILLARY WRINKLING
Iyer, N., Cooper, K., Yang, J., et al. (2008) · AIP conference proceedings · doi:10.1063/1.2989066 · Cited by 2 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Yee, C., Dickson, K.-A., Muntasir, M. N., Ma, Y., & Marsh, D. J. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.836984 · Cited by 66 · see citing works
- Authors
- Fan, R., Piou, M., Darling, E., Cormier, D., Sun, J., & Wan, J.
- Affiliations
- Rochester Institute of Technology (US)
- University of Illinois Chicago (US)
- Corresponding author
- Wan, Jiandi
- Venue
- Journal of Biomaterials Applications
- Publisher
- SAGE Publications
- Published
- 2016-09-25
- Volume
- 31
- Issue
- 5
- Pages
- 684-692
- ISSN
- 0885-3282 (ISSN-L)
- 1530-8022
- PMC
- PMC5603305
- OpenAlex
- W2520866530
- Cited by
- 132
- References
- 37
- Citations by year
- 2026: 6 · 2025: 14 · 2024: 9 · 2023: 16 · 2022: 16 · 2021: 22 · 2020: 15 · 2019: 11 · 2018: 16 · 2017: 7
- FWCI
- 5.46
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Biomaterials Applications (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- Swim Across America
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK105118)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- 3D bioprinting, Matrigel, agarose, hybrid hydrogels, bioinks, cell-laden constructs, HCT116 cells, intestinal epithelium, rheological properties, biocompatibility, tissue engineering
- MeSH terms
- Biocompatible Materials, Cell Line, Cell Survival, Collagen, Drug Combinations, Epithelial Cells, Humans, Laminin, Proteoglycans, Sepharose, Hydrogels, Tissue Engineering, Cell Proliferation, Batch Cell Culture Techniques, Bioprinting, Printing, Three-Dimensional
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
