Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels
Open accessAbstract
Fabrication of three dimensional (3D) organoids with controlled microarchitectures has been shown to enhance tissue functionality. Bioprinting can be used to precisely position cells and cell-laden materials to generate controlled tissue architecture. Therefore, it represents an exciting alternative for organ fabrication. Despite the rapid progress in the field, the development of printing processes that can be used to fabricate macroscale tissue constructs from ECM-derived hydrogels has remained a challenge. Here we report a strategy for bioprinting of photolabile cell-laden methacrylated gelatin (GelMA) hydrogels. We bioprinted cell-laden GelMA at concentrations ranging from 7 to 15% with varying cell densities and found a direct correlation between printability and the hydrogel mechanical properties. Furthermore, encapsulated HepG2 cells preserved cell viability for at least eight days following the bioprinting process. In summary, this work presents a strategy for direct-write bioprinting of a cell-laden photolabile ECM-derived hydrogel, which may find widespread application for tissue engineering, organ printing and the development of 3D drug discovery platforms.
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By adapting this method, the authors avoided potentially harsh printing conditions including low temperature for a prolonged printing period and undesirable shear stress along the length of the nozzle.
“By adapting this method, we avoided potentially harsh printing conditions including, low temperature for a prolonged printing period [51] and undesirable shear stress along the length of the nozzle [30].”
From A liver-on-a-chip platform with bioprinted hepatic spheroids (2016) · cites this paper as [30]
Section 3.3 reports that cell viability was not significantly affected and suggests the hydrogel matrix may protect cells from shear stress during dispensing. It also says dispensing required significant stress, so the source supports protection from its effects, not avoidance of shear stress along the nozzle. The low-temperature condition is attached to a different citation in the citing sentence. (LLM, conf=0.84)
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3D Bioprinting in Skeletal Muscle Tissue Engineering
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Print Me An Organ! Why We Are Not There Yet
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Self-citationPrintability–A key issue in extrusion-based bioprinting
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3D neural tissue models: From spheroids to bioprinting
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3D Bioprinting of Vessel-like Structures with Multilevel Fluidic Channels
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Multiscale bioprinting of vascularized models
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Self-citationPrinting Technologies for Medical Applications
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Polymeric Systems for Bioprinting
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Bioinks and bioprinting: A focused review
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A brief review of extrusion‐based tissue scaffold bio‐printing
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Biomaterials-based 3D cell printing for next-generation therapeutics and diagnostics
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Review of 3D printable hydrogels and constructs
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Nanoengineered Ionic–Covalent Entanglement (NICE) Bioinks for 3D Bioprinting
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Extrusion bioprinting of soft materials: An emerging technique for biological model fabrication
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Three-Dimensional Bioprinting for Regenerative Dentistry and Craniofacial Tissue Engineering
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Self-citation3D bioprinting and itsin vivoapplications
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3D Bioprinting for Vascularized Tissue Fabrication
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UV-Assisted 3D Bioprinting of Nanoreinforced Hybrid Cardiac Patch for Myocardial Tissue Engineering
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3D bioprinting of photocrosslinkable hydrogel constructs
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Evaluation of bioink printability for bioprinting applications
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Tissue Engineering Applications of Three-Dimensional Bioprinting
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3D printed microchannel networks to direct vascularisation during endochondral bone repair
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Extrusion and Microfluidic‐Based Bioprinting to Fabricate Biomimetic Tissues and Organs
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Self-citationEmergence of FRESH 3D printing as a platform for advanced tissue biofabrication
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Application of 3D Printing for Smart Objects with Embedded Electronic Sensors and Systems
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Three-dimensional bioprinting of gelatin methacryloyl (GelMA)
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Structural analysis of photocrosslinkable methacryloyl-modified protein derivatives
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Self-citationNatural and Synthetic Bioinks for 3D Bioprinting
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Microfluidic organ-on-a-chip models of human liver tissue
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Hyaluronic acid as a bioink for extrusion-based 3D printing
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3D Bioprinted cancer models: Revolutionizing personalized cancer therapy
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Ophthalmic gels: Past, present and future
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(Photo-)crosslinkable gelatin derivatives for biofabrication applications
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Osteogenic and angiogenic tissue formation in high fidelity nanocomposite Laponite-gelatin bioinks
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Recent advances in high-strength and elastic hydrogels for 3D printing in biomedical applications
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- Authors
- Bertassoni, L. E., Cardoso, J. C., Manoharan, V., Cristino, A. L., Bhise, N. S., Araujo, W. A., Zorlutuna, P., Vrana, N. E., Ghaemmaghami, A. M., Dokmeci, M. R., & Khademhosseini, A.
- Affiliations
- Brigham and Women's Hospital (US)
- University of Nottingham (GB)
- Corresponding author
- Dokmeci, Mehmet R
- Khademhosseini, Ali
- ORCID
- Venue
- Biofabrication
- Publisher
- IOP Publishing
- Published
- 2014-04-03
- Volume
- 6
- Issue
- 2
- Pages
- 024105
- ISSN
- 1758-5082 (ISSN-L)
- 1758-5090
- PMC
- PMC4040163
- OpenAlex
- W2100081159
- Cited by
- 642
- References
- 37
- Citations by year
- 2026: 17 · 2025: 36 · 2024: 34 · 2023: 44 · 2022: 55 · 2021: 69 · 2020: 72 · 2019: 80 · 2018: 74 · 2017: 68 · 2016: 56 · 2015: 32 · 2014: 4
- FWCI
- 27.67
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biofabrication (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Repository@Nottingham (University of Nottingham) (submittedVersion, cc-by)
- Repository@Nottingham (University of Nottingham) (submittedVersion)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- National Institutes of Health (EB02597)
- Defense Threat Reduction Agency
- Biotechnology and Biological Sciences Research Council (BB/I02643X/1)
- Australian Research Council (DP120104837)
- National Heart, Lung, and Blood Institute (HL-099073, R01HL099073)
- National Institute of General Medical Sciences (R01GM095906, GM095906)
- National Institute of Allergy and Infectious Diseases (R01AI081534, AI081534)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR057837, R01AR057837)
- National Institute of Biomedical Imaging and Bioengineering (EB02597)
- National Institute of Dental and Craniofacial Research (DE021468, R01DE021468)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- gelatin methacryloyl, cell-laden hydrogels, 3D organoids, printability, hydrogel mechanical properties, HepG2 cells, cell viability, tissue engineering, organ printing
- MeSH terms
- Animals, Biocompatible Materials, Cell Survival, Gelatin, Humans, Methacrylates, Hydrogels, Tissue Engineering, NIH 3T3 Cells, Mice, Tissue Scaffolds, Elastic Modulus, Hep G2 Cells, Bioprinting
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