The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
ICAM-1 on exosomes from mature dendritic cells is critical for efficient naive T-cell priming
Abstract
Exosomes are secreted vesicles formed in late endocytic compartments. Immature dendritic cells (DCs) secrete exosomes, which transfer functional major histocompatibility complex (MHC)–peptide complexes to other DCs. Since immature and mature DCs induce different functional T-cell responses (ie, tolerance versus priming), we asked whether DC maturation also influenced the priming abilities of their exosomes. We show that exosomes secreted by lipopolysaccharide (LPS)–treated mature DCs are 50- to 100-fold more potent to induce antigen-specific T-cell activation in vitro than exosomes from immature DCs. In vitro, exosomes from mature DCs transfer to B lymphocytes the ability to prime naive T cells. In vivo, only mature exosomes trigger effector T-cell responses, leading to fast skin graft rejection. Proteomic and biochemical analyses revealed that mature exosomes are enriched in MHC class II, B7.2, intercellular adhesion molecule 1 (ICAM-1), and bear little milk-fat globule–epidermal growth factor–factor VIII (MFG-E8) as compared with immature exosomes. Functional analysis using DC-derived exosomes from knock-out mice showed that MHC class II and ICAM-1 are required for mature exosomes to prime naive T cells, whereas B7.2 and MFG-E8 are dispensable. Therefore, changes in protein composition and priming abilities of exosomes reflect the maturation signals received by DCs.
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Cites
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Exosomes: composition, biogenesis and function
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Self-citationIdentification and proteomic profiling of exosomes in human urine
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Antigen Presentation and T Cell Stimulation by Dendritic Cells
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Self-citationIdentification of a factor that links apoptotic cells to phagocytes
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Exosomal-like vesicles are present in human blood plasma
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Self-citationEndocytosis, intracellular sorting, and processing of exosomes by dendritic cells
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Cited by
Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007) · Nature Cell Biology · doi:10.1038/ncb1596 · Cited by 12,834
Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles
Colombo, M., Raposo, G., & Théry, C. (2014) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-101512-122326 · Cited by 6,186
Self-citationBiological properties of extracellular vesicles and their physiological functions
Yáñez‐Mó, M., Siljander, P. R. ‐., Andreu, Z., Bedina Zavec, A., Borràs, F. E., Buzas, E. I., Buzas, K., Casal, E., Cappello, F., Carvalho, J., et al. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27066 · Cited by 5,926
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Self-citationKowal, J., Arras, G., Colombo, M., Jouve, M., Morath, J. P., Primdal-Bengtson, B., Dingli, F., Loew, D., Tkach, M., & Théry, C. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1521230113 · Cited by 3,426
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Metadata
- Authors
- Segura, E., Nicco, C., Lombard, B., Véron, P., Raposo, G., Batteux, F., Amigorena, S., & Théry, C.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2005-07-01
- Volume
- 106
- Issue
- 1
- Pages
- 216-223
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1974209230
- Cited by
- 613
- References
- 40
- FWCI
- 7.61
- Type
- journal article
- Language
- en
- Open access
- closed
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