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Persistence of naive CD45RA+ regulatory T cells in adult life
Open accessAbstract
Regulatory T cells (TREGs) constitutively expressing CD4, CD25, and the transcription factor Foxp3 can prevent a wide range of experimental and spontaneous autoimmune diseases in mice. In humans, CD4+CD25bright T cells, predominantly within the CD45RO+ activated/memory subset in adults and the CD45RA+ naive T-cell subset in infants, are considered to be the equivalent subset. Using novel combinations of monoclonal antibodies (mAbs), we examined expression of CD25 in human infant thymus, cord blood, adult peripheral blood, lymph node, and spleen. In addition to the CD4+CD25bright T cells, subfractionation on the basis of CD45 splice variants indicated that all samples contained a second distinct population of cells expressing a slightly lower level of CD25. In adult peripheral blood, this population expressed a naive CD45RA+ phenotype. The corresponding population in lymph node, spleen, and cord blood showed some evidence of activation, and expressed markers characteristic of TREGs, such as cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). Sorted CD4+CD25+CD45RA+ T cells from both cord and adult blood expressed very high levels of mRNA for Foxp3 and manifested equivalent suppressive activity in vitro, indicating that they are bone fide members of the regulatory T-cell lineage. Targeting naive TREGs in adults may offer new means of preventing and treating autoimmune disease.
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“with naive-like Treg cells decreasing and memory-like Treg cells increasing with age”
From Successful and Maladaptive T Cell Aging (2017) · cites this paper as Seddiki et al., 2006
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Metadata
- Authors
- Seddiki, N., Santner-Nanan, B., Tangye, S. G., Alexander, S. I., Solomon, M., Lee, S., Nanan, R., & de Saint Groth, B. F.
- Affiliations
- The University of Sydney (AU)
- From the Centenary Institute of Cancer Medicine and Cell Biology, Faculty of Medicine, University of Sydney, NSW, Australia; Department of Pediatrics, University of Sydney, Western Clinical School, Penrith, NSW, Australia; The Childrens Hospital at Westmead, NSW, Australia; and the Departments of Gastroenterology and Pathology, Royal Prince Alfred Hospital, Camperdown, NSW, Australia.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2006-04-01
- Volume
- 107
- Issue
- 7
- Pages
- 2830-2838
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2067263905
- Cited by
- 284
- References
- 50
- Citations by year
- 2026: 8 · 2025: 10 · 2024: 4 · 2023: 6 · 2022: 9 · 2021: 6 · 2020: 12 · 2019: 10 · 2018: 7 · 2017: 13 · 2016: 11 · 2015: 12 · 2014: 12 · 2013: 21 · 2012: 13
- FWCI
- 6.27
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Crohn's and Colitis Foundation of America
- Cancer Australia
- National Health and Medical Research Council
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- regulatory T cells, CD25 expression, Foxp3, CD45RO+ memory T cells, CTLA-4, Treg suppressive function, umbilical cord blood, thymus, peripheral blood, lymph nodes, spleen, autoimmune disease prevention
- MeSH terms
- Aging, Animals, Humans, Lymphocyte Activation, Mice, Inbred C57BL, T-Lymphocytes, CD4-Positive T-Lymphocytes, Leukocyte Common Antigens, Mice, Knockout, Homeodomain Proteins, Mice
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