The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Unique effects of Stat3 on the early phase of hematopoietic stem cell regeneration
Abstract
Self-renewal of hematopoietic stem cells (HSCs) is key to their reconstituting ability, but the signaling pathways that regulate this process remain poorly understood. Here we show that transduction of adult mouse bone marrow cells with a constitutively activated form of Stat3 (Stat3-C) increased their regenerative activity in lethally irradiated recipients. Conversely, transduction of these cells with a dominant-negative form of Stat3 suppressed their regenerative activity. Serial transplantation and clonal tracking of the HSC progeny regenerated in vivo from STAT3-C-transduced HSCs demonstrated that the major effect of forced expression of STAT3-C was to enhance HSC self-renewal during the initial phase of hematopoietic recovery. This acquired potential for enhanced self-renewal divisions then became latent, but was reactivated when the cells were transferred to new irradiated recipients. Increased levels of activated STAT3 were also found to be associated with greater preservation of primitive hematopoietic cells in short-term cultures. These results indicate a novel biphasic regulation of HSC self-renewal in vivo in which activated STAT3 promotes HSC self-renewal under stimulated, but not homeostatic, conditions. STAT3 may thus be an important regulator of hematopoietic regeneration and a novel target for HSC engineering.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Unaltered basal hematopoiesis; impaired stem cell regeneration after 5-FU”
From Targeting Janus Kinases and Signal Transducer and Activator of Transcription 3 To Treat Inflammation, Fibrosis, and Cancer: Rationale, Progress, and Caution (2020) · cites this paper as Chung et al., 2006
Citations
Cites
Bromberg, J. F., Wrzeszczynska, M. H., Devgan, G., Zhao, Y., Pestell, R. G., Albanese, C., & Darnell, J. E. (1999) · Cell · doi:10.1016/s0092-8674(00)81959-5 · Cited by 2,790
A role for Wnt signalling in self-renewal of haematopoietic stem cells
Reya, T., Duncan, A. W., Ailles, L., Domen, J., Scherer, D. C., Willert, K., Hintz, L., Nusse, R., & Weissman, I. L. (2003) · Nature · doi:10.1038/nature01593 · Cited by 2,159
Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells.
Park, I.-K., Qian, D., Kiel, M., Becker, M. W., Pihalja, M., Weissman, I. L., Morrison, S. J., & Clarke, M. F. (2003) · Nature · doi:10.1038/nature01587 · Cited by 1,871
TRANSCRIPTIONAL RESPONSES TO POLYPEPTIDE LIGANDS: The JAK-STAT Pathway
Schindler, C., & Darnell, J. E. (1995) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.64.070195.003201 · Cited by 1,808
"Stemness": Transcriptional Profiling of Embryonic and Adult Stem Cells
Ramalho‐Santos, M., Yoon, S., Matsuzaki, Y., Mulligan, R. C., & Melton, D. A. (2002) · Science · doi:10.1126/science.1072530 · Cited by 1,711
Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3Preprint
Niwa, H., Burdon, T., Chambers, I., & Smith, A. (1998) · Genes & Development · doi:10.1101/gad.12.13.2048 · Cited by 1,588
A Stem Cell Molecular Signature
Ivanova, N. B., Dimos, J. T., Schaniel, C., Hackney, J. A., Moore, K. A., & Lemischka, I. R. (2002) · Science · doi:10.1126/science.1073823 · Cited by 1,543
Bmi-1 determines the proliferative capacity of normal and leukaemic stem cells.
Lessard, J., & Sauvageau, G. (2003) · Nature · doi:10.1038/nature01572 · Cited by 1,452
Hematopoietic Stem Cell Quiescence Maintained by p21 cip1/waf1
Cheng, T., Rodrigues, N., Shen, H., Yang, Y.-G., Dombkowski, D., Sykes, M., & Scadden, D. T. (2000) · Science · doi:10.1126/science.287.5459.1804 · Cited by 1,339
Developmental potential and dynamic behavior of hematopoietic stem cells
Lemischka, I. R., Raulet, D. H., & Mulligan, R. C. (1986) · Cell · doi:10.1016/0092-8674(86)90566-0 · Cited by 848
Levy, D. E., & Lee, C. (2002) · Journal of Clinical Investigation · doi:10.1172/jci15650 · Cited by 834
The Stat family in cytokine signaling
Ihle, J. N. (2001) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(00)00199-x · Cited by 713
HOXB4-Induced Expansion of Adult Hematopoietic Stem Cells Ex Vivo
Antonchuk, J., Sauvageau, G., & Humphries, R. K. (2002) · Cell · doi:10.1016/s0092-8674(02)00697-9 · Cited by 700
Dick, J. E., Magli, M. C., Huszar, D., Phillips, R. A., & Bernstein, A. (1985) · Cell · doi:10.1016/s0092-8674(85)80102-1 · Cited by 637
Sauvageau, G., Þorsteinsdóttir, U., Eaves, C. J., Lawrence, H. J., Largman, C., Lansdorp, P. M., & Humphries, R. K. (1995) · Genes & Development · doi:10.1101/gad.9.14.1753 · Cited by 586
Self-citationTebbutt, N. C., Giraud, A. S., Inglese, M., Jenkins, B., Waring, P., Clay, F. J., Malki, S., Alderman, B. M., Grail, D., Hollande, F., et al. (2002) · Nature Medicine · doi:10.1038/nm763 · Cited by 474
Szilvassy, S. J., Humphries, R. K., Lansdorp, P. M., Eaves, A. C., & Eaves, C. J. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.22.8736 · Cited by 450
Self-citationO’Farrell, A.-M., Liu, Y., Moore, K. W., & Mui, A. L. ‐. (1998) · The EMBO Journal · doi:10.1093/emboj/17.4.1006 · Cited by 440
Essential role of STAT3 for embryonic stem cell pluripotency
Raz, R., Lee, C., Cannizzaro, L. A., D’Eustachio, P., & Levy, D. E. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.6.2846 · Cited by 407
Welte, T., Zhang, S. S. M., Wang, T., Zhang, Z., Hesslein, D. G. T., Yin, Z., Kano, A., Iwamoto, Y., Li, E., Craft, J. E., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0237137100 · Cited by 381
Rapid activation of the stat3 transcription complex in liver regeneration
Cressman, D. E., Diamond, R. H., & Taub, R. (1995) · Hepatology · doi:10.1002/hep.1840210531 · Cited by 314
From hematopoiesis to neuropoiesis: Evidence of overlapping genetic programs
Terskikh, A. V., Easterday, M. C., Li, L., Hood, L., Kornblum, H. I., Geschwind, D. H., & Weissman, I. L. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.131200898 · Cited by 288
Activation of gp130 Transduces Hypertrophic Signals via STAT3 in Cardiac Myocytes
Kunisada, K., Tone, E., Fujio, Y., Matsui, H., Yamauchi‐Takihara, K., & Kishimoto, T. (1998) · Circulation · doi:10.1161/01.cir.98.4.346 · Cited by 236
HER‐2/ neu Signal Transduction in Human Breast and Ovarian Cancer
Reese, D. M., & Slamon, D. J. (1997) · STEM CELLS · doi:10.1002/stem.150001 · Cited by 229
Serine Phosphorylation and Negative Regulation of Stat3 by JNK
Lim, C. P., & Cao, X. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.43.31055 · Cited by 225
Hematopoietic stem cells expand during serial transplantation in vivo without apparent exhaustion
Iscove, N. N., & Nawa, K. (1997) · Current Biology · doi:10.1016/s0960-9822(06)00341-1 · Cited by 214
Repression of Stat3 activity by activation of mitogen-activated protein kinase (MAPK)
Jain, N., Zhang, T., Fong, S., Lim, C. P., & Cao, X. (1998) · Oncogene · doi:10.1038/sj.onc.1202238 · Cited by 213
Kato, Y., Iwama, A., Tadokoro, Y., Shimoda, K., Minoguchi, M., Akira, S., Tanaka, M., Miyajima, A., Kitamura, T., & Nakauchi, H. (2005) · The Journal of Experimental Medicine · doi:10.1084/jem.20042541 · Cited by 184
Notch signalling in hematopoiesis
Ohishi, K., Katayama, N., Shiku, H., Varnum‐Finney, B., & BERNSTEIN, I. (2003) · Seminars in Cell and Developmental Biology · doi:10.1016/s1084-9521(02)00183-0 · Cited by 173
Þorsteinsdóttir, U., Sauvageau, G., & Humphries, R. K. (1999) · Blood · doi:10.1182/blood.v94.8.2605.420k19_2605_2612 · Cited by 154
Audet, J., Miller, C., Rose‐John, S., Piret, J. M., & Eaves, C. J. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.98.4.1757 · Cited by 137
Self-citationCombined interleukin 6 and soluble interleukin 6 receptor accelerates murine liver regeneration
Peters, M., Blinn, G., Jostock, T., Schirmacher, P., Büschenfelde, K. M. Z., Galle, P. R., & Rose–John, S. (2000) · Gastroenterology · doi:10.1053/gast.2000.20236 · Cited by 129
Ser727-dependent transcriptional activation by association of p300 with STAT3 upon IL-6 stimulation
Schuringa, J.-J., Schepers, H., Vellenga, E., & Kruijer, W. (2001) · FEBS Letters · doi:10.1016/s0014-5793(01)02354-7 · Cited by 124
Roles of Stat3 and ERK in G‐CSF Signaling
Kamezaki, K., Shimoda, K., Numata, A., Haro, T., Kakumitsu, H., Yoshie, M., Yamamoto, M., Takeda, K., Matsuda, T., Akira, S., et al. (2005) · Stem Cells · doi:10.1634/stemcells.2004-0173a · Cited by 66
Oh, I., & Eaves, C. J. (2002) · Oncogene · doi:10.1038/sj.onc.1205592 · Cited by 57
Self-citationHematopoietic stem cells: Inferences from in vivo assays
Eaves, C. J., Miller, C., Cashman, J. D., Conneally, E., Petzer, A. L., Zandstra, P. W., & Eaves, A. (1997) · Stem Cells · doi:10.1002/stem.5530150802 · Cited by 50
Self-citationOntogenic emergence of definitive hematopoietic stem cells
Dzierzak, E. A. (2003) · Current Opinion in Hematology · doi:10.1097/00062752-200305000-00006 · Cited by 49
Hematopoietic Stem Cell Protocols
(2002) · Methods in molecular medicine · doi:10.1385/159259140x · Cited by 42
Szilvassy, S. J., Nicolini, F. E., Eaves, C. J., & Miller, C. (2003) · Humana Press eBooks · doi:10.1385/1-59259-140-x:167 · Cited by 25
Self-citationOh, I., Fabry, M. E., Humphries, R. K., et al. (2004) · Experimental Hematology · doi:10.1016/j.exphem.2004.02.001 · Cited by 23
Self-citation
Research works printed but not linked: 2
- 1997, Stem Cells., 15, 1
- Schuringa JJ, Schepers H, Vellenga E, Kruijer W. Ser727-dependent transcriptional activation by association of p300 with STAT3 upon IL-6 stimulation. FEBS Lett.2001;495: 71-76. · DOI · Paper page
Cited by
Sorted by citations
Bharadwaj, U., Kasembeli, M. M., Robinson, P., & Tweardy, D. J. (2020) · Pharmacological Reviews · doi:10.1124/pr.119.018440 · Cited by 314
STAT3, stem cells, cancer stem cells and p63
Galoczová, M., Coates, P. J., & Vojtěšek, B. (2018) · Cellular & Molecular Biology Letters · doi:10.1186/s11658-018-0078-0 · Cited by 272
Foshay, K. M., & Gallicano, G. I. (2008) · Developmental Biology · doi:10.1016/j.ydbio.2008.11.016 · Cited by 226
Dendritic cell (DC)-specific targeting reveals Stat3 as a negative regulator of DC function
Melillo, J. A., Song, L., Bhagat, G., Blazquez, A. B., Plumlee, C. R., Lee, C., Berin, C., Reizis, B., & Schindler, C. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.0902960 · Cited by 225
Small Molecule Inhibitors of Signal Transducer and Activator of Transcription 3 (Stat3) Protein
Debnath, B., Xu, S., & Neamati, N. (2012) · Journal of Medicinal Chemistry · doi:10.1021/jm300207s · Cited by 206
Metadata
- Authors
- Chung, Y.-J., Park, B.-B., Kang, Y.-J., Kim, T.-M., Eaves, C. J., & Oh, I.-H.
- Affiliations
- BC Cancer Agency (CA)
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2006-08-15
- Volume
- 108
- Issue
- 4
- Pages
- 1208-1215
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2166180289
- Semantic Scholar
- 5d35bdca0b6f9dfa9fac5e3966d0a3779f0ec0a0
- Cited by
- 107
- References
- 40
- Citations by year
- 2025: 4 · 2024: 1 · 2023: 4 · 2022: 5 · 2021: 4 · 2020: 7 · 2019: 5 · 2018: 7 · 2017: 7 · 2016: 7 · 2015: 5 · 2014: 6 · 2013: 9 · 2012: 4
- FWCI
- 5.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
- Keywords
- STAT3, hematopoietic stem cells, self-renewal, hematopoietic regeneration, STAT3 activation, dominant-negative STAT3, bone marrow transplantation, irradiation, clonal tracking, serial transplantation
- MeSH terms
- Animals, Cell Division, Cells, Cultured, Enzyme Activation, Gene Expression Regulation, Hematopoietic Stem Cells, Mice, Transgenic, Regeneration, Transduction, Genetic, Whole-Body Irradiation, Signal Transduction, Bone Marrow Transplantation, STAT3 Transcription Factor, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review