The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Multiple vitamin K-dependent coagulation zymogens promote adenovirus-mediated gene delivery to hepatocytes
Open accessAbstract
Upon local delivery, adenovirus (Ad) serotype 5 viruses use the coxsackie and Ad receptor (CAR) for cell binding and αv integrins for internalization. When administered systemically, however, their role in liver tropism is limited because CAR-permissive and mutated viruses show similar biodistribution, a finding recently attributed to blood coagulation factor (F) IX or complement protein C4BP binding to the adenovirus fiber and “bridging” to either low-density lipoprotein receptor-related protein or heparan sulfate proteoglycans. Here, we show that hepatocyte transduction in vitro can be enhanced by the vitamin K-dependent factors FX, protein C, and FVII in addition to FIX but not by prothrombin (FII), FXI, and FXII. This phenomenon was not dependent on proteolytic activation or cell signaling activity and for FX was mediated by direct virus-factor binding. Human FX substantially enhanced hepatocyte transduction by CAR-permissive and mutated viruses in an ex vivo liver perfusion model. In vivo, global down-regulation of vitamin K-dependent zymogens by warfarin significantly diminished liver uptake of CAR-deleted Ads; however, this phenomenon was fully rescued by acute infusion of human FX. Our results indicate a common and pivotal role for distinct vitamin K-dependent coagulation factors in mediating hepatocyte transduction by adenoviruses in vitro and in vivo.
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Cites
Isolation of a Common Receptor for Coxsackie B Viruses and Adenoviruses 2 and 5
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Self-citationBlood clearance rates of adenovirus type 5 in mice
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Engineering targeted viral vectors for gene therapy
Waehler, R., Russell, S. J., & Curiel, D. T. (2007) · Nature Reviews Genetics · doi:10.1038/nrg2141 · Cited by 687
Adenovirus serotype 5 hexon is critical for virus infection of hepatocytes in vivo
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Self-citationCurrent Advances and Future Challenges in Adenoviral Vector Biology and Targeting
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The AAV9 receptor and its modification to improve in vivo lung gene transfer in mice
Bell, C. L., Vandenberghe, L. H., Bell, P., Limberis, M. P., Gao, G.-P., Van Vliet, K., Agbandje-McKenna, M., & Wilson, J. M. (2011) · Journal of Clinical Investigation · doi:10.1172/jci57367 · Cited by 190
Metadata
- Authors
- Parker, A. L., Waddington, S. N., Nicol, C. G., Shayakhmetov, D. M., Buckley, S. M., Denby, L., Kemball-Cook, G., Ni, S., Lieber, A., McVey, J. H., Nicklin, S. A., & Baker, A. H.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2006-10-15
- Volume
- 108
- Issue
- 8
- Pages
- 2554-2561
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1967033604
- Cited by
- 297
- References
- 32
- FWCI
- 14.49
- Type
- journal article
- Language
- en
- Open access
- green
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- Parker, A. L., Waddington, S. N., Nicol, C. G., Shayakhmetov, D. M., Buckley, S. M., Denby, L., Kemball-Cook, G., Ni, S., Lieber, A., McVey, J. H., Nicklin, S. A., & Baker, A. H. (2006). Multiple vitamin K-dependent coagulation zymogens promote adenovirus-mediated gene delivery to hepatocytes. Blood. https://doi.org/10.1182/blood-2006-04-008532
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