The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Generation of functional platelets from human embryonic stem cells in vitro via ES-sacs, VEGF-promoted structures that concentrate hematopoietic progenitors
Open accessAbstract
Human embryonic stem cells (hESCs) could potentially represent an alternative source for blood transfusion therapies and a promising tool for studying the ontogeny of hematopoiesis. When we cultured hESCs on either C3H10T1/2 or OP-9 cells to facilitate hematopoiesis, we found that exogenous administration of vascular endothelial growth factor promoted the emergence of sac-like structures, which we named embryonic stem cell–derived sacs (ES-sacs). These ES-sacs consisted of multiple cysts demarcated by cellular monolayers that retained some of the properties of endothelial cells. The spherical cells inside ES-sacs expressed primarily CD34, along with VE-cadherin, CD31, CD41a, and CD45, and were able to form hematopoietic colonies in semisolid culture and to differentiate into mature megakaryocytes by day 24 in the presence of thrombopoietin. Apparently, ES-sacs provide a suitable environment for hematopoietic progenitors. Relatively large numbers of mature megakaryocytes could be induced from the hematopoietic progenitors within ES-sacs, which were then able to release platelets that displayed integrin αIIbβ3 activation and spreading in response to ADP or thrombin. This novel protocol thus provides a means of generating platelets from hESCs, which could serve as the basis for efficient production of platelets for clinical transfusion and studies of thrombopoiesis.
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Cites
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Cited by
Ditadi, A., Sturgeon, C. M., Tober, J., Awong, G., Kennedy, M., Yzaguirre, A. D., Azzola, L., Ng, E. S., Stanley, E. G., French, D. L., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3161 · Cited by 320
Ex vivo generation of platelet products from human iPS cells
Nakamura, S., Sugimoto, N., & Eto, K. (2020) · Inflammation and Regeneration · doi:10.1186/s41232-020-00139-2 · Cited by 16
Self-citation
Metadata
- Authors
- Takayama, N., Nishikii, H., Usui, J., Tsukui, H., Sawaguchi, A., Hiroyama, T., Eto, K., & Nakauchi, H.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2008-06-01
- Volume
- 111
- Issue
- 11
- Pages
- 5298-5306
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2005744447
- Cited by
- 317
- References
- 33
- FWCI
- 11.64
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Takayama, N., Nishikii, H., Usui, J., Tsukui, H., Sawaguchi, A., Hiroyama, T., Eto, K., & Nakauchi, H. (2008). Generation of functional platelets from human embryonic stem cells in vitro via ES-sacs, VEGF-promoted structures that concentrate hematopoietic progenitors. Blood. https://doi.org/10.1182/blood-2007-10-117622
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