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Cited by 71Blood disorders and treatmentsNeutropenia and Cancer InfectionsNeutrophil, Myeloperoxidase and Oxidative Mechanisms

The DOI matches its Crossref record.

Assessing the delivery of neutrophils to tissues in neutropenia

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Wright, DG, Meierovics, AI, Foxley, JM·1986·Blood
DOI 10.1182/blood.v67.4.1023.1023
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Abstract

Studies of neutrophil kinetics in neutropenic individuals, as well as clinical observations of variability in the occurrence of infection among patients with neutropenia, have suggested that blood neutrophil counts may not uniformly reflect the effective delivery of neutrophils to extravascular tissues where the cells perform their principal host defense functions. To evaluate this possibility we developed a sensitive, reproducible method of measuring the extravascular delivery of neutrophils to a normal mucosal site of neutrophil turnover. This method is based upon the quantification of neutrophils recoverable from saline mouth wash specimens. Twenty-five mL specimens, obtained in a controlled manner from neutropenic patients and normal subjects, were centrifuged and the sediments resuspended in 1.0 mL Hank's buffer with 2 micrograms acridine orange, incubated at 37 degrees C for 15 minutes, and then examined in a hemocytometer chamber by fluorescence microscopy. Neutrophils could be clearly distinguished by their characteristic fluorescence and were counted. With this method as few as 1,500 neutrophils were detected reliably in mouth wash specimens. Mucosal neutrophil counts varied less than 10% with repeated sampling of individual subjects over 5-day periods and were consistently greater than 1.3 X 10(5)/specimen in non-neutropenic individuals. Although profound neutropenia was generally reflected by lower than normal oral mucosal neutrophil counts, these counts were significantly higher in individuals with chronic severe neutropenia (blood neutrophils less than 300/mm3) than in patients with acute neutropenia of comparable severity that had developed following chemotherapy. Also, in individuals recovering from profound neutropenia, neutrophils usually reappeared earlier in mouth wash specimens than in blood, and oral mucosal neutrophil counts attained recovery levels more rapidly than did blood counts. This phenomenon was particularly evident in an individual with cyclic neutropenia. Moreover, mucosal neutrophils could occasionally be detected in profoundly neutropenic patients when neutrophils were not present in blood samples. These findings indicate that mucosal neutrophil counts in individuals with neutropenia provide information about the delivery of neutrophils to tissues that may not be apparent from blood neutrophil counts alone.

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  • The recruitment of neutrophils by the oral biofilm may serve as a non-invasive measure of in vivo systemic neutrophil activation and chemotaxis.

    “The recruitment of neutrophils by the oral biofilm may serve as a non-invasive measure of in vivo systemic neutrophil activation and chemotaxis [13-15].”

    From Oral neutrophils are an independent marker of the systemic inflammatory response after cardiac bypass (2014) · cites this paper as [13]

    The paper describes a non-invasive saline mouth-wash method for counting oral mucosal neutrophils and says the counts reflect delivery of neutrophils to tissues (Abstract; page not supplied). It does not establish that oral biofilm recruits those cells or that the count measures systemic neutrophil activation or chemotaxis. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

  • Neutrophil tissue repopulation following hematopoietic stem cell transplantation (HSCT) confers protection from infection.

    “Neutrophil tissue repopulation following hematopoietic stem cell transplantation (HSCT) confers protection from infection [13];”

    From Oral neutrophils are an independent marker of the systemic inflammatory response after cardiac bypass (2014) · cites this paper as [13]

    The paper reports that neutrophils reappeared in the oral mucosa during recovery from chemotherapy-induced neutropenia, and its discussion describes tissue neutrophils as contributing to host defense. It does not establish that tissue repopulation after HSCT protects against infection; the discussion explicitly cautions that the study was not designed to assess infection risk. The indexed passages do not supply a reliable page number. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • Research suggests that the oral cavity may provide an early opportunity to non-invasively monitor the innate immune system.

    “Research suggests that the oral cavity may provide an early opportunity to non-invasively monitor the innate immune system [13-15].”

    From Oral neutrophils are an independent marker of the systemic inflammatory response after cardiac bypass (2014) · cites this paper as [13]

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Metadata

Authors
Wright, D., Meierovics, A., & Foxley, J.
Affiliations
Walter Reed Army Institute of Research (US)
Venue
Blood
Publisher
American Society of Hematology
Published
1986-04-01
Volume
67
Issue
4
Pages
1023-1030
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
DOI
10.1182/blood.v67.4.1023.1023
OpenAlex
W1656770909
Cited by
71
References
0
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2021: 1 · 2020: 2 · 2019: 4 · 2016: 1 · 2015: 3 · 2014: 1 · 2013: 2 · 2012: 7
FWCI
0.74
Type
journal article
Language
en
Open access
Locations
  • Blood (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Hematology › Blood disorders and treatments
  • Health Sciences › Medicine › Oncology › Neutropenia and Cancer Infections
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Keywords
neutropenia, neutrophil kinetics, cyclic neutropenia, chemotherapy-induced neutropenia, neutrophil recovery, infection susceptibility
MeSH terms
Acute Disease, Adult, Agranulocytosis, Cell Movement, Cell Separation, Chronic Disease, Female, Humans, Leukocyte Count, Male, Middle Aged, Mouth Mucosa, Neutropenia, Neutrophils

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