The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages
Abstract
Pluripotent hematopoietic stem cells have been studied extensively, but the events that occur during their differentiation remain largely uncharted. To develop a system that allows the differentiation of cultured multipotent progenitors by time-lapse fluorescence microscopy, myelomonocytic cells were labeled with green fluorescent protein (GFP) in vivo. This was achieved by knocking the enhanced GFP (EGFP) gene into the murine lysozyme M (lys) locus and using a targeting vector, which contains a neomycin resistant (neo) gene flanked by LoxP sites and “splinked” ends, to increase the frequency of homologous recombination. Analysis of the blood and bone marrow of thelys-EGFP mice revealed that most myelomonocytic cells, especially mature neutrophil granulocytes, were fluorescence-positive, while cells from other lineages were not. Removal of the neogene through breeding of the mice with the Cre-deleter strain led to an increased fluorescence intensity. Mice with an inactivation of both copies of the lys gene developed normally and were fertile.
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The embryonic cell lineage of the nematode Caenorhabditis elegans
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Metadata
- Authors
- Faust, N., Varas, F., Kelly, L. M., Heck, S., & Graf, T.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2000-07-15
- Volume
- 96
- Issue
- 2
- Pages
- 719-726
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W175241905
- Cited by
- 748
- References
- 2
- FWCI
- 2.79
- Type
- journal article
- Language
- en
- Open access
- closed
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