The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
The variable number of tandem repeats element in DAT1 regulates in vitro dopamine transporter density
Open accessAbstract
Background: A 40-bp variable number of tandem repeats (VNTR) polymorphism exists in the 15 th exon of DAT1, the gene encoding the human dopamine transporter (DAT). Though the VNTR resides in a region encoding the 3' untranslated region (UTR) and does not alter the protein's amino acid sequence, the prevalent 10-repeat variant has shown both linkage and association to Attention Deficit Hyperactivity Disorder (ADHD). In this study, we examined the effects of the DAT1 VNTR on measures of in vitro DAT expression and pharmacology. A series of four DAT1 constructs, each containing the DAT1 coding region, but varying with respect to the downstream presence or content of the 3'UTR, were engineered and stably transfected into an HEK-293 variant using Flp-In integration, an enzyme-mediated, site-specific recombination technology. Results: [3 H] Win 35,428 saturation binding assays and DAT immunoblots revealed statistically significant differences in DAT expression attributable to DAT1 genotype. Cells harboring the 10-repeat DAT1 variant were characterized by a B max approximately 50% greater than cells with the 9-repeat VNTR; those containing only the DAT1 coding region or the coding region flanked by a truncated 3' UTR resulted in greater DAT density than either of the naturalistic 9- and 10-repeat variants. Competition binding assays showed no statistically significant DAT1 genotype effects on the DAT affinity for methylphenidate, a finding consistent with the positional location of the VNTR. Conclusion: This study identified the DAT1 VNTR as a functional polymorphism and provides an interpretive framework for its association with behavioral phenotypes.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Association of attention-deficit disorder and the dopamine transporter gene.
Cook, E. H., Stein, M. A., Krasowski, M. D., Cox, N. J., Olkon, D. M., Kieffer, J. E., & LEVENTHAL, B. L. (1995) · PubMed · Cited by 996
Untranslated regions of mRNAs.
(2002) · Genome biology · doi:10.1186/gb-2002-3-3-reviews0004 · Cited by 987
Human dopamine transporter gene (DAT1) maps to chromosome 5p15.3 and displays a VNTR
Vandenbergh, D. J., Persico, A. M., Hawkins, A. L., Griffin, C. A., Li, X., Jabs, E. W., & Uhl, G. R. (1992) · Genomics · doi:10.1016/s0888-7543(05)80138-7 · Cited by 708
Cloning and Expression of a Cocaine-Sensitive Dopamine Transporter Complementary DNA
Shimada, S., Kitayama, S., Lin, C.-L., Patel, A., Nanthakumar, E., Gregor, P., Kuhar, M., & Uhl, G. (1991) · Science · doi:10.1126/science.1948034 · Cited by 586
Recombinase-Mediated Gene Activation and Site-Specific Integration in Mammalian Cells
O'Gorman, S., Fox, D. T., & Wahl, G. M. (1991) · Science · doi:10.1126/science.1900642 · Cited by 572
Genotype Influences In Vivo Dopamine Transporter Availability in Human Striatum
Heinz, A. (2000) · Neuropsychopharmacology · doi:10.1016/s0893-133x(99)00099-8 · Cited by 538
The 3′ untranslated region of messenger RNA: A molecular ‘hotspot’ for pathology?
Conne, B., Stutz, A., & Vassalli, J.-D. (2000) · Nature Medicine · doi:10.1038/76211 · Cited by 530
Gill, M., Daly, G., Heron, S., Hawi, Z., & Fitzgerald, M. (1997) · Molecular Psychiatry · doi:10.1038/sj.mp.4000290 · Cited by 482
Giros, B., el Mestikawy, S., Godinot, N., Zheng, K., Han, H., Yang-Feng, T., & Caron, M. G. (1992) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)08925-4 · Cited by 477
Waldman, I. D., Rowe, D. C., Abramowitz, A., Kozel, S. T., Mohr, J. H., Sherman, S. L., Cleveland, H. H., Sanders, M. L., Gard, J. M. C., & Stever, C. (1998) · The American Journal of Human Genetics · doi:10.1086/302132 · Cited by 462
Cited by
Insights into the neural basis of response inhibition from cognitive and clinical neuroscience
Chambers, C. D., Garavan, H., & Bellgrove, M. A. (2009) · Neuroscience & Biobehavioral Reviews · doi:10.1016/j.neubiorev.2008.08.016 · Cited by 938
Tripp, G., & Wickens, J. R. (2009) · Neuropharmacology · doi:10.1016/j.neuropharm.2009.07.026 · Cited by 492
Althof, S. E., McMahon, C. G., Waldinger, M. D., Serefoglu, E. C., Shindel, A. W., Adaikan, P. G., Becher, E., Dean, J., Giuliano, F., Hellstrom, W. J. G., et al. (2014) · Sexual Medicine · doi:10.1002/sm2.28 · Cited by 444
Forbes, E. E., Brown, S. M., Kimak, M., Ferrell, R. E., Manuck, S. B., & Hariri, A. R. (2009) · Molecular Psychiatry · doi:10.1038/sj.mp.4002086 · Cited by 408
Variation in dopamine genes influences responsivity of the human reward system
Dreher, J.-C., Kohn, P., Kolachana, B., Weinberger, D. R., & Berman, K. F. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0805517106 · Cited by 376
Metadata
- Authors
- VanNess, S. H., Owens, M. J., & Kilts, C. D.
- Venue
- BMC Genetics
- Published
- 2005-11-27
- Volume
- 6
- Issue
- 1
- Pages
- 55-55
- ISSN
- 1471-2156 (ISSN-L)
- OpenAlex
- W2140009084
- Cited by
- 306
- References
- 49
- FWCI
- 5.89
- Type
- journal article
- Language
- en
- Open access
- gold
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: The variable number of tandem repeats element in DAT1 regulates in vitro dopamine transporter density. https://app.cochat.ai/papers/10.1186/1471-2156-6-55
- The paper
- VanNess, S. H., Owens, M. J., & Kilts, C. D. (2005). The variable number of tandem repeats element in DAT1 regulates in vitro dopamine transporter density. BMC Genetics. https://doi.org/10.1186/1471-2156-6-55
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
