Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli

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Modulating the Precursor and Terpene Synthase Supply for the Whole-Cell Biocatalytic Production of the Sesquiterpene (+)-Zizaene in a Pathway Engineered E. coli

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Concepts for flow chemistry with whole-cell biocatalysts

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Expression and characterization of Trichoderma virens UKM-1 endochitinase in Escherichia coli

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Generation of biologically active recombinant human OCT4 protein from E. coli

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Characterization of Sphingobacterium sp. Ab3 Lipase and Its Coexpression with LEA Peptides

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Co-production of GroELS discriminates between intrinsic and thermally-induced recombinant protein aggregation during substrate quality control

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l-Arabinose triggers its own uptake via induction of the arabinose-specific Gal2p transporter in an industrial Saccharomyces cerevisiae strain

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Atypical effect of temperature tuning on the insertion of the catalytic iron−sulfur center in a recombinant [FeFe]‐hydrogenase

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Strain engineering and process optimization for enhancing the production of a thermostable steryl glucosidase in Escherichia coli

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Identifying genomic targets for protein over-expression by “omics” analysis of Quiescent Escherichia coli cultures

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Improving the soluble expression of aequorin in Escherichia coli using the chaperone-based approach by co-expression with artemin

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A new approach for analyzing an adhesive bacterial protein in the mouse gastrointestinal tract using optical tissue clearing

Nishiyama, K., Sugiyama, M., Yamada, H., Makino, K., Ishihara, S., Takaki, T., Mukai, T., & Okada, N. (2019) · Scientific Reports · doi:10.1038/s41598-019-41151-y · Cited by 12

Screening and characterization of Acidiphilium sp. PJH and its role in bioleaching

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Cellular response to accumulation of recombinant proteins in the E. coli inner membrane: Implications for proteolysis and productivity of the secretory expression system

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A high-throughput screen for polysialyltransferase activity

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CFD simulation of mass transfer and flow behaviour around a single particle in bioleaching process

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Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

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CopySwitch—in vivo Optimization of Gene Copy Numbers for Heterologous Gene Expression in Bacillus subtilis

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Rational Design of the Soluble Variant of l -Pipecolic Acid Hydroxylase using the α-Helix Rule and the Hydropathy Contradiction Rule

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Genetically engineered Escherichia coli FBR5: Part I. Comparison of high cell density bioreactors for enhanced ethanol production from xylose

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Immunogenicity of IMS 1113 plus soluble subunit and chimeric proteins containingMycoplasma hyopneumoniaeP97 C-terminal repeat regions

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High Yield Production of a Soluble Bifidobacterial β-Galactosidase (BbgIV) in E. coli DH5α with Improved Catalytic Efficiency for the Synthesis of Prebiotic Galactooligosaccharides

Osman, A., Tzortzis, G., Rastall, R. A., & Charalampopoulos, D. (2013) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf304792g · Cited by 11

Generation of a Recombinant Stem Cell-Specific Human SOX2 Protein from Escherichia coli Under Native Conditions

Thool, M., Dey, C., Bhattacharyya, S., Selvaraju, S., & Thummer, R. P. (2021) · Molecular Biotechnology · doi:10.1007/s12033-021-00305-y · Cited by 11

RSM-based Model to Predict Optimum Fermentation Conditions for Soluble Expression of the Antibody Fragment Derived from 4D5MOC-B Humanized Mab in SHuffle™ T7 E. coli.

Behravan, A., & Hashemi, A. (2021) · PubMed Central · doi:10.22037/ijpr.2020.114377.14822 · Cited by 11

Recombinant alpha-fetoprotein receptor-binding domain co-expression with polyglutamate tags facilitates in vivo folding in E. coli

Mollaev, M. D., Gorokhovets, N. V., Nikolskaya, E. D., Faustova, M., Zabolotskii, A. I., Sokol, M. B., Tereshenko, O., Zhunina, O. A., Shvets, V. I., Severin, E. S., et al. (2017) · Protein Expression and Purification · doi:10.1016/j.pep.2017.11.001 · Cited by 11

VirB10 vaccination for protection against Anaplasma phagocytophilum

Crosby, F. L., Lundgren, A. M., Hoffman, C. A., Pascual, D. W., & Barbet, A. F. (2018) · BMC Microbiology · doi:10.1186/s12866-018-1346-x · Cited by 11

The signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host’s fluorescent intensity

Gao, J., Qian, H., Guo, X., Mi, Y. M., Guo, J., Zhao, J., Xu, C., Zheng, T., Duan, M., Tang, Z., et al. (2020) · Microbial Cell Factories · doi:10.1186/s12934-020-01371-8 · Cited by 11

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

McCormick, A. M., Jarmusik, N. A., Endrizzi, E. J., & Leipzig, N. D. (2014) · Journal of Visualized Experiments · doi:10.3791/51295 · Cited by 11

Cloning and expression of a full-length glutamate decarboxylase gene from a high-yielding γ-aminobutyric acid yeast strain MJ2

Jiang, D., Cai, Q., Gao, A., Li, J., Yang, Y., Xu, X., Ye, Y., & Hou, J. (2012) · Annals of Microbiology · doi:10.1007/s13213-012-0493-9 · Cited by 11

Structural properties of the HNF-1A transactivation domain

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Effect of the respiro-fermentative balance during yeast propagation on fermentation and wort attenuation

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Heterologous production of active form of beta-lytic protease by Bacillus subtilis and improvement of staphylolytic activity by protein engineering

Hioki, T., Yamashita, D., Tohata, M., Endo, K., Kawahara, A., & Okuda, M. (2021) · Microbial Cell Factories · doi:10.1186/s12934-021-01724-x · Cited by 10

Treatment of simulated biomass gasification wastewater of varying strength in a three stage rotating biological contactor

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Efficient production of 2H, 13C, 15N-enriched industrial enzyme Rhizopus chinensis lipase with native disulfide bonds

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Enhanced effects of DNA vaccine against botulinum neurotoxin serotype A by targeting antigen to dendritic cells

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Cloning, expression and characterization of a HER2-alpha luffin fusion protein inEscherichia coli

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High-Level Expression and Substrate-Binding Region Modification of a Novel BL312 Milk-Clotting Enzyme To Enhance the Ratio of Milk-Clotting Activity to Proteolytic Activity

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Analysis of Methods to Improve the Solubility of Recombinant Bovine Sex Determining Region Y Protein.

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Targeting the cell wall: Preparation of monoclonal antibody for accurate identification of Alicyclobacillus acidoterrestris in apple juice

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Purifying and Characterizing Bacterially Expressed Soluble Lactate Dehydrogenase from Plasmodium knowlesi for the Development of Anti-Malarial Drugs

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Heterologous expression, purification, and characterization of cytochrome P450sca-2 and mutants with improved solubility in Escherichia coli

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High-Level Expression of Functionally Active Dengue-2 Non-Structural Antigen 1 Production inEscherichia coli

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Refolding Your Protein with a Little Help from REFOLD

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Rapid enzyme regeneration results in the striking catalytic longevity of an engineered, single species, biocatalytic biofilm

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Biosorption Strategies in the Remediation of Toxic Pollutants from Contaminated Water Bodies

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ReToAd: simple method for the rapid replacement of promoters to improve protein production

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Expression of Cell-Penetrating Peptides Fused to Protein Cargo

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Functional Expression of a Mo–Cu-Dependent Carbon Monoxide Dehydrogenase (CODH) and Its Use as a Dissolved CO Bio-microsensor

Reginald, S. S., Etzerodt, M., Fapyane, D., & Chang, I. S. (2021) · ACS Sensors · doi:10.1021/acssensors.1c01243 · Cited by 10

Protein Expression Plasmids Produced Rapidly: Streamlining Cloning Protocols and Robotic Handling

Kornienko, M. A., Montalvo, A., Carpenter, B. E., Lenard, M., Abeywickrema, P. D., Hall, D. L., Darke, P. L., & Kuo, L. C. (2005) · Assay and Drug Development Technologies · doi:10.1089/adt.2005.3.661 · Cited by 10

Modifications of the 5’ region of the CASPONTM tag’s mRNA further enhance soluble recombinant protein production in Escherichia coli

Köppl, C., Buchinger, W., Striedner, G., & Cserjan‐Puschmann, M. (2024) · Microbial Cell Factories · doi:10.1186/s12934-024-02350-z · Cited by 10

Serine carboxypeptidases from the carnivorous plant Nepenthes mirabilis: Partial characterization and heterologous expression

Porfírio, C. T. M. N., Souza, P. F. N., Ramos, M. V., Campos, F., Freitas, S. F., de Oliveira, J. P. B., Furtado, G. P., Barbosa, J. S. S., Frota, T. L., Nagano, C. S., et al. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.12.104 · Cited by 9

High‐level expression and purification of soluble bioactive recombinant human heparin‐binding epidermal growth factor in Escherichia coli

Khalili, M., Soleyman, M. R., Baazm, M., & Beyer, C. (2015) · Cell Biology International · doi:10.1002/cbin.10454 · Cited by 9

Disulfide Bond Formation and Activation of Escherichia coli β-Galactosidase under Oxidizing Conditions

Seras‐Franzoso, J., Affentranger, R., Ferrer‐Navarro, M., Daura, X., Villaverde, A., & García‐Fruitós, E. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.06923-11 · Cited by 9

Evaluation of in vitro refolding vs cold shock expression: Production of a low yielding single chain variable fragment

Vermeulen, J., Burt, F. J., van Heerden, E., Cason, E. D., & Meiring, M. (2018) · Protein Expression and Purification · doi:10.1016/j.pep.2018.06.005 · Cited by 9

Bacterial Production of Barley Stripe Mosaic Virus Biotemplates for Palladium Nanoparticle Growth

Lee, Y., Lee, K. Z., Susler, R. G., Scott, C. A., Wang, L., Loesch‐Fries, L. S., Harris, M. T., & Solomon, K. (2020) · ACS Applied Nano Materials · doi:10.1021/acsanm.0c02570 · Cited by 9

Metallization and investigation of electrical properties ofin vitrorecrystallized mSbsC-eGFP assemblies

Korkmaz, N., Börrnert, F., Köhler, D., Mendes, R. G., Bachmatiuk, A., Rümmeli, M. H., Büchner, B., Eng, L. M., & Rödel, G. (2011) · Nanotechnology · doi:10.1088/0957-4484/22/37/375606 · Cited by 9

Synthesis and expression of recombinant interferon alpha-5 gene in tobacco chloroplasts, a non-edible plant

Khan, M. S., & Nurjis, F. (2011) · Molecular Biology Reports · doi:10.1007/s11033-011-1227-y · Cited by 9

Direct and simple detection of recombinant proteins from cell lysates using differential scanning fluorimetry

Seo, D., Jung, J., Kim, H., & Park, C. (2013) · Analytical Biochemistry · doi:10.1016/j.ab.2013.09.027 · Cited by 9

Analysis of the Expression and Activity of Nitric Oxide Synthase from Marine Photosynthetic Microorganisms

Foresi, N. P., Correa‐Aragunde, N., Santolini, J., & Lamattina, L. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-3600-7_13 · Cited by 9

Electroinduced Extraction of Human Ferritin Heavy Chain Expressed in Hansenula polymorpha

Ganeva, V., Galutzov, B., Angelova, B., & Suckow, M. (2017) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-017-2627-9 · Cited by 9

Genetic and Biochemical Characterization of a Novel Thermostable Cyclomaltodextrinase From Anoxybacillus flavithermus

Aliakbari, N., Mirzaee, Z., Jafarian, V., Khalifeh, K., & Salehi, M. (2018) · Starch - Stärke · doi:10.1002/star.201800133 · Cited by 9

Overview of Protein Expression Vectors for E. coli

Riggs, P. D. (2018) · Current Protocols Essential Laboratory Techniques · doi:10.1002/cpet.23 · Cited by 10

Protein-based direct reprogramming of fibroblasts to neuronal cells using 30Kc19 protein and transcription factor Ascl1

Ryu, J., Hwang, N. S.-Y., Park, H. H., & Park, T. H. (2020) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2020.105717 · Cited by 9

Generation of a recombinant version of a biologically active cell-permeant human HAND2 transcription factor from E. coli

Haridhasapavalan, K. K., Sundaravadivelu, P. K., Joshi, N., Das, N. J., Mohapatra, A., Udayashree, V., Kaveeshwar, V., & Thummer, R. P. (2022) · Scientific Reports · doi:10.1038/s41598-022-19745-w · Cited by 9

Optimization of Conditions for Expression of Dengue Serotype 2 EDIII Protein in Escherichia coli and Immune Responses of Adjuvant-Free EDIII Ferritin Nanoparticles Against Dengue Virus in BALB/c Mice

Tennakoon, M. S. B. W. T. M. N. S., Lee, K. H., Lee, H., Park, J., & Shin, H. (2025) · Viruses · doi:10.3390/v17010129 · Cited by 8

Computational design, expression, and characterization of a Plasmodium falciparum multi-epitope, multi-stage vaccine candidate (PfCTMAG)

Chick, J. A., Abongdia, N. N., Shey, R. A., & Apinjoh, T. O. (2025) · Heliyon · doi:10.1016/j.heliyon.2025.e42014 · Cited by 8

Assessing the clinical and bacteriological outcomes of vaccination with recombinant Asp14 and OmpA against A. phagocytophilum in sheep

Eskeland, S., Stuen, S., Crosby, F. L., Lybeck, K. R., Barbet, A. F., Lindgren, P., Tollefsen, S., Wilhelmsson, P., Tollersrud, T., Makvandi‐Nejad, S., et al. (2019) · Veterinary Immunology and Immunopathology · doi:10.1016/j.vetimm.2019.109936 · Cited by 8

Protein Production and Purification of a Codon-Optimized Human NGN3 Transcription Factor from E. coli

Narayan, G., Agrawal, A., Joshi, N., Gogoi, R., Nagotu, S., & Thummer, R. P. (2021) · The Protein Journal · doi:10.1007/s10930-021-10020-x · Cited by 8

Characterization and application of a recombinant dopa decarboxylase from Harmonia axyridis for the efficient biosynthesis of dopamine

Gao, S., Guo, Y., Ma, C., Ma, D., Chen, K., Ouyang, P., & Wang, X. (2021) · Chinese Journal of Chemical Engineering · doi:10.1016/j.cjche.2021.12.006 · Cited by 8

Simultaneous Production of a Virus-Like Particle Linked to dsRNA to Enhance dsRNA Delivery for Yellow Head Virus Inhibition

Worawittayatada, J., Angsujinda, K., Sinnuengnong, R., Attasart, P., Smith, D. R., & Assavalapsakul, W. (2022) · Viruses · doi:10.3390/v14122594 · Cited by 8

Antimicrobial peptidase lysostaphin at subinhibitory concentrations modulates staphylococcal adherence, biofilm formation, and toxin production

Yuan, Y., Chen, K., Sun, C., Ahmed, S., & Ojha, S. C. (2023) · BMC Microbiology · doi:10.1186/s12866-023-03052-z · Cited by 8

High cell density co-culture for production of recombinant hydrolases

Silva, M. R., Severo, M. G., Delabona, P. D. S., Ruller, R., Pradella, J. G. D. C., Gonçalves, V. M., & Freitas, S. (2012) · Biochemical Engineering Journal · doi:10.1016/j.bej.2012.11.008 · Cited by 8

Low molecular weight liquid media development for Lactobacilli producing bacteriocins

Zacharof, M., & Lovitt, R. W. (2012) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.3892 · Cited by 8

Dissection of an old protein reveals a novel application: domain D of Staphylococcus aureus Protein A (sSpAD) as a secretion - tag

Heel, T., Paal, M., Schneider, R., & Auer, B. (2010) · Microbial Cell Factories · doi:10.1186/1475-2859-9-92 · Cited by 8

Influence of polycations on antibacterial activity of lysostaphin

Kulikov, S. N., Хайруллин, Р. З., & Варламов, В. В. (2015) · Applied Biochemistry and Microbiology · doi:10.1134/s0003683815060083 · Cited by 8

Cellular acclimation of Acidithiobacillus ferrooxidans to sulfur biooxidation

Zhang, C., Xia, J. L., Ding, J., Ouyang, X., Nie, Z.-Y., & Qiu, G. (2009) · Mining Metallurgy & Exploration · doi:10.1007/bf03403415 · Cited by 8

Functional characterization of host toxic EcdB transcription factor protein of echinocandin B biosynthetic gene cluster

Kumar, A., Kumar, V., & Kumar, A. (2019) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.1763 · Cited by 8

Explanatory Chapter: Troubleshooting Protein Expression

Duong‐Ly, K. C., & Gabelli, S. B. (2014) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-420119-4.00018-5 · Cited by 8

Antibacterial activity of a dual peptide targeting the Escherichia coli sliding clamp and the ribosome

André, C., Veillard, F., Wolff, P., Lobstein, A.-M., Compain, G., Monsarrat, C., Reichhart, J., Noûs, C., Burnouf, D., Guichard, G., et al. (2020) · RSC Chemical Biology · doi:10.1039/d0cb00060d · Cited by 8

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Nguyen, T. K. O., Vu, T. L., Nguyen, M. Q., Ta, H. K. K., Park, K. S., Kim, S. H., Kim, C. J., Jang, Y. J., & Choe, H. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22105267 · Cited by 8

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Compartment-specific metabolome labeling enables the identification of subcellular fluxes that may serve as promising metabolic engineering targets in CHO cells

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Generation of a transducible version of a bioactive recombinant human TBX5 transcription factor from E. Coli

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Optimization of EnBase Fed-Batch Cultivation to Improve Soluble Fraction Ratio of α-Luffin Ribosome Inactivating Protein

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Design of a chitosan-based nano vaccine against epsilon toxin of Clostridium perfringens type D and evaluation of its immunogenicity in BALB/c mice

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Microbial Cell Factories BioMed Central Review Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli

Sørensen, H. P., & Mortensen, K. K. (2005)

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Doyle, S. A. (2004) · eScholarship (California Digital Library)

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