In utero exposure to a low concentration of diesel exhaust affects spontaneous locomotor activity and monoaminergic system in male mice
Open accessAbstract
Background: Epidemiological studies have suggested that suspended particulate matter (SPM) causes detrimental health effects such as respiratory and cardiovascular diseases, and that diesel exhaust particles from automobiles is a major contributor to SPM. It has been reported that neonatal and adult exposure to diesel exhaust damages the central nervous system (CNS) and induces behavioral alteration. Recently, we have focused on the effects of prenatal exposure to diesel exhaust on the CNS. In this study, we examined the effects of prenatal exposure to low concentration of diesel exhaust on behaviour and the monoaminergic neuron system. Spontaneous locomotor activity (SLA) and monoamine levels in the CNS were assessed. Methods: Mice were exposed prenatally to a low concentration of diesel exhaust (171 μg DEP/m 3) for 8 hours/day on gestational days 2-16. SLA was assessed for 3 days in 4-week-old mice by analysis of the release of temperature-associated infrared rays. At 5 weeks of age, the mice were sacrificed and the brains were used for analysis by high-performance liquid chromatography (HPLC). Results and Discussion: Mice exposed to a low concentration of diesel exhaust showed decreased SLA in the first 60 minutes of exposure. Over the entire test period, the mice exposed prenatally to diesel exhaust showed decreased daily SLA compared to that in control mice, and the SLA in each 3 hour period was decreased when the lights were turned on. Neurotransmitter levels, including dopamine and noradrenaline, were increased in the prefrontal cortex (PFC) in the exposure group compared to the control group. The metabolites of dopamine and noradrenaline also increased in the PFC. Neurotransmitter turnover, an index of neuronal activity, of dopamine and noradrenaline was decreased in various regions of the CNS, including the striatum, in the exposure group. The serum corticosterone level was not different between groups. The data suggest that decreased SLA in mice exposed prenatally to diesel exhaust is due to facilitated release of dopamine in the PFC. Conclusions: These results indicate that exposure of mice in utero to a low concentration of diesel exhaust decreases SLA and alters the neurochemical monoamine metabolism of several regions of the brain.
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Altered locomotor effects after PM exposure signify the role of dopamine pathway activation and glycine signaling inhibition.
“Findings related to altered locomotor effects also signify the role of dopamine pathway activation, as well as glycine signaling inhibition [112, 117].”
From Air pollution and children’s health—a review of adverse effects associated with prenatal exposure from fine to ultrafine particulate matter (2021) · cites this paper as [112]
The Discussion (page not specified) reports decreased locomotor activity after prenatal diesel-exhaust exposure and suggests facilitated dopamine release in the prefrontal cortex as a possible explanation. The Results (page not specified) also report increased prefrontal dopamine, but the paper does not support the glycine-signaling inhibition part of the claim or establish a general effect of PM exposure. (LLM, conf=0.87)
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- Authors
- Suzuki, T., Oshio, S., Iwata, M., Saburi, H., Odagiri, T., Udagawa, T., Sugawara, I., Umezawa, M., & Takeda, K.
- Affiliations
- Tokyo University of Science (JP)
- Japan Science and Technology Agency (JP)
- Corresponding author
- Takeda, Ken
- Venue
- Particle and Fibre Toxicology
- Published
- 2010-03-23
- Volume
- 7
- Issue
- 1
- Pages
- 7-7
- ISSN
- 1743-8977 (ISSN-L)
- PMC
- PMC2853486
- OpenAlex
- W2111730860
- Cited by
- 145
- References
- 3
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 7 · 2023: 9 · 2022: 16 · 2021: 15 · 2020: 6 · 2019: 11 · 2018: 5 · 2017: 12 · 2016: 12 · 2015: 13 · 2014: 5 · 2013: 7 · 2012: 8
- FWCI
- 6.86
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Particle and Fibre Toxicology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology (CREST)
- University of Tsukuba
- Hoshi University
- Japan Science and Technology Agency (CREST, 332-0012)
- Core Research for Evolutional Science and Technology
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Prenatal Substance Exposure Effects
- Keywords
- diesel exhaust particles, prenatal exposure, spontaneous motor activity, monoaminergic systems, dopamine, norepinephrine, prefrontal cortex, striatum, neurotransmitter turnover, dopamine metabolites, noradrenaline metabolites, central nervous system, behavioral effects, air pollution, particulate matter
- MeSH terms
- Air Pollutants, Animals, Vehicle Emissions, Behavior, Animal, Brain, Brain Chemistry, Catecholamines, Chromatography, High Pressure Liquid, Female, Locomotion, Male, Mice, Inbred ICR, Pregnancy, Prenatal Exposure Delayed Effects, Maternal Exposure, Inhalation Exposure, Mice
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