Polycomblike protein PHF1b: a transcriptional sensor for GABA receptor activity
Open accessAbstract
Background: The γ-aminobutyric acid (GABA) type A receptor (GABA A R) contains the recognition sites for a variety of agents used in the treatment of brain disorders, including anxiety and epilepsy. A better understanding of how receptor expression is regulated in individual neurons may provide novel opportunities for therapeutic intervention. Towards this goal we have studied transcription of a GABA A R subunit gene (GABRB1) whose activity is autologously regulated by GABA via a 10 base pair initiator-like element (β 1-INR). Methods: By screening a human cDNA brain library with a yeast one-hybrid assay, the Polycomblike (PCL) gene product PHD finger protein transcript b (PHF1b) was identified as a β 1-INR associated protein. Promoter/reporter assays in primary rat cortical cells demonstrate that PHF1b is an activator at GABRB1, and chromatin immunoprecipitation assays reveal that presence of PHF1 at endogenous Gabrb1 is regulated by GABA A R activation. Results: PCL is a member of the Polycomb group required for correct spatial expression of homeotic genes in Drosophila. We now show that PHF1b recognition of β 1-INR is dependent on a plant homeodomain, an adjacent helix-loop-helix, and short glycine rich motif. In neurons, it co-immunoprecipitates with SUZ12, a key component of the Polycomb Repressive Complex 2 (PRC2) that regulates a number of important cellular processes, including gene silencing via histone H3 lysine 27 trimethylation (H3K27me3). Conclusions: The observation that chronic exposure to GABA reduces PHF1 binding and H3K27 monomethylation, which is associated with transcriptional activation, strongly suggests that PHF1b may be a molecular transducer of GABA A R function and thus GABA-mediated neurotransmission in the central nervous system.
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The PHF1b isoform promotes transcription of GABRB1, which encodes GABA type A receptor, resulting in regulation of GABA-mediated neurotransmission in the central nervous system, in particular in neocortical and hippocampal neurons.
“the PHF1b isoform promotes the transcription of GABRB1, which encodes GABA type A receptor (GABAAR); this results in the regulation of GABA-mediated neurotransmission in the central nervous system, in particular in neocortical and hippocampal neurons92.”
From Distinct selective forces and Neanderthal introgression shaped genetic diversity at genes involved in neurodevelopmental disorders (2017) · cites this paper as [92]
The Background and Discussion report that PHF1b stimulates GABRB1 promoter activity, and the Results show PHF1 binding at the Gabrb1 promoter in rat neocortical and hippocampal neurons. But GABRB1 encodes a GABA-A receptor β1 subunit, not the entire receptor. The Conclusions say PHF1b *may* transduce GABA-A receptor activity and thus affect CNS neurotransmission; they do not establish the claimed downstream regulation as a demonstrated result. (LLM, conf=0.87)
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Cites
The language of covalent histone modifications
Strahl, B. D., & Allis, C. D. (2000) · Nature · doi:10.1038/47412 · Cited by 8,679 · see citing works
The Polycomb complex PRC2 and its mark in life
Margueron, R., & Reinberg, D. (2011) · Nature · doi:10.1038/nature09784 · Cited by 3,347 · see citing works
The “initiator” as a transcription control element
Smale, S. T., & Baltimore, D. (1989) · Cell · doi:10.1016/0092-8674(89)90176-1 · Cited by 1,572 · see citing works
Rost, B., Yachdav, G., & Liu, J. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh377 · Cited by 1,462 · see citing works
GAL4-VP16 is an unusually potent transcriptional activator
Sadowski, I., Ma, J., Triezenberg, S., & Ptashne, M. (1988) · Nature · doi:10.1038/335563a0 · Cited by 1,298 · see citing works
Laboratory course manual for methods in yeast genetics
Sherman, F., Fink, G. R., & Hicks, J. B. (1986) · Cold Spring Harbor Laboratory eBooks · Cited by 1,231 · see citing works
Laurie, D., Wisden, W., & Seeburg, P. (1992) · Journal of Neuroscience · doi:10.1523/jneurosci.12-11-04151.1992 · Cited by 1,144 · see citing works
The RNA Polymerase II Core Promoter
Smale, S. T., & Kadonaga, J. T. (2003) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.72.121801.161520 · Cited by 1,138 · see citing works
The molecular genetics of embryonic pattern formation in Drosophila
Ingham, P. W. (1988) · Nature · doi:10.1038/335025a0 · Cited by 1,049 · see citing works
The general transcription factors of RNA polymerase II.Preprint
Orphanides, G. M., Lagrange, T., & Reinberg, D. (1996) · Genes & Development · doi:10.1101/gad.10.21.2657 · Cited by 1,036 · see citing works
Shi, Y., Seto, E., Chang, L., & Shenk, T. E. (1991) · Cell · doi:10.1016/0092-8674(91)90189-6 · Cited by 1,035 · see citing works
The role of general initiation factors in transcription by RNA polymerase II
ROEDER, R. (1996) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(96)10050-5 · Cited by 1,009 · see citing works
Deletion analysis of GAL4 defines two transcriptional activating segments
Ma, J., & Ptashne, M. (1987) · Cell · doi:10.1016/0092-8674(87)90081-x · Cited by 887 · see citing works
The PHD finger: Implications for chromatin-mediated transcriptional regulation
Aasland, R. (1995) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(00)88957-4 · Cited by 850 · see citing works
Xia, Z., Dudek, H., Miranti, C. K., & Greenberg, M. E. (1996) · Journal of Neuroscience · doi:10.1523/jneurosci.16-17-05425.1996 · Cited by 794 · see citing works
Brooks‐Kayal, A. R., Shumate, M. D., Jin, H., Rikhter, T. Y., & Coulter, D. A. (1998) · Nature Medicine · doi:10.1038/2661 · Cited by 746 · see citing works
GABAergic Excitation Promotes Neuronal Differentiation in Adult Hippocampal Progenitor Cells
Tozuka, Y., Fukuda, S., Namba, T., Seki, T., & Hisatsune, T. (2005) · Neuron · doi:10.1016/j.neuron.2005.08.023 · Cited by 741 · see citing works
(2009) · Nucleic Acids Research · doi:10.1093/nar/gkp1184 · Cited by 680 · see citing works
Kuo, M., & Allis, C. D. (1999) · Methods · doi:10.1006/meth.1999.0879 · Cited by 601 · see citing works
Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery
Hampsey, M. (1998) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.62.2.465-503.1998 · Cited by 561 · see citing works
Gozani, O., Karuman, P., Jones, D. R., Ivanov, D., Cha, J., Lugovskoy, A. A., Baird, C. L., Zhu, H., Field, S. J., Lessnick, S. L., et al. (2003) · Cell · doi:10.1016/s0092-8674(03)00480-x · Cited by 487 · see citing works
From ion currents to genomic analysis: Recent advances in GABAA receptor research
Rabow, L. E., Russek, S. J., & Farb, D. H. (1995) · Synapse · doi:10.1002/syn.890210302 · Cited by 469 · see citing works
Self-citationUnlocking the mechanisms of transcription factor YY1: are chromatin modifying enzymes the key?
Thomas, M. J. N., & Seto, E. (1999) · Gene · doi:10.1016/s0378-1119(99)00261-9 · Cited by 463 · see citing works
Regulation of Transcription Factor YY1 by Acetylation and Deacetylation
Yao, Y., Yang, W., & Seto, E. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.17.5979-5991.2001 · Cited by 431 · see citing works
Brown, J. L., Mucci, D., Whiteley, M., Dirksen, M., & Kassis, J. A. (1998) · Molecular Cell · doi:10.1016/s1097-2765(00)80106-9 · Cited by 415 · see citing works
THE POLYCOMB AND TRITHORAX GROUP PROTEINS OF DROSOPHILA: Trans-Regulators of Homeotic Gene Function
Kennison, J. A. (1995) · Annual Review of Genetics · doi:10.1146/annurev.ge.29.120195.001445 · Cited by 395 · see citing works
Polycomb, Epigenomes, and Control of Cell Identity
Orlando, V. (2003) · Cell · doi:10.1016/s0092-8674(03)00157-0 · Cited by 339 · see citing works
Rastelli, L., Chan, C. S., & Pirrotta, V. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb05795.x · Cited by 314 · see citing works
Binding of TAFs to core elements directs promoter selectivity by RNA polymerase II
Verrijzer, C. P., Chen, J., Yokomori, K., & Tjian, R. (1995) · Cell · doi:10.1016/s0092-8674(05)80016-9 · Cited by 287 · see citing works
Ezh2 Requires PHF1 To Efficiently Catalyze H3 Lysine 27 Trimethylation In Vivo
Sarma, K., Margueron, R., Ivanov, A. V., Pirrotta, V., & Reinberg, D. (2008) · Molecular and Cellular Biology · doi:10.1128/mcb.02017-07 · Cited by 286 · see citing works
Locking in stable states of gene expression: transcriptional control during Drosophila development
Simon, J. A. (1995) · Current Opinion in Cell Biology · doi:10.1016/0955-0674(95)80093-x · Cited by 279 · see citing works
Chromatin dynamics at DNA replication, transcription and repair
Ehrenhofer‐Murray, A. E. (2004) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.2004.04162.x · Cited by 275 · see citing works
The Chromatin Remodeler Mi-2β Is Required for CD4 Expression and T Cell Development
Williams, C. J., Naito, T., Arco, P. G., Seavitt, J. R., Cashman, S. M., Souza, B. D., Qi, X., Keables, P., von Andrian, U. H., & Georgopoulos, K. (2004) · Immunity · doi:10.1016/j.immuni.2004.05.005 · Cited by 266 · see citing works
Genome-Wide Prediction of Polycomb/Trithorax Response Elements in Drosophila melanogaster
Ringrose, L., Rehmsmeier, M., Dura, J., & Paro, R. (2003) · Developmental Cell · doi:10.1016/s1534-5807(03)00337-x · Cited by 261 · see citing works
Chalkley, G. E., & Verrijzer, C. P. (1999) · The EMBO Journal · doi:10.1093/emboj/18.17.4835 · Cited by 250 · see citing works
Rost, B. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg508 · Cited by 247 · see citing works
Tora, L. (2002) · Genes & Development · doi:10.1101/gad.976402 · Cited by 231 · see citing works
A TATA box implicated in E1A transcriptional activation of a simple adenovirus 2 promoter
Wu, L. L., Rosser, D. S. E., Schmidt, M. C., et al. (1987) · Nature · doi:10.1038/326512a0 · Cited by 227 · see citing works
PIPs: human protein-protein interaction prediction database
McDowall, M. D., Scott, M. S., & Barton, G. J. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn870 · Cited by 224 · see citing works
PcG complexes and chromatin silencing
Pirrotta, V. (1997) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(97)80135-9 · Cited by 219 · see citing works
Transcription factor YY1 functions as a PcG protein in vivo
Atchison, L., Ghias, A., Wilkinson, F. H., Bonini, N. M., & Atchison, M. L. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg124 · Cited by 209 · see citing works
Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA.
Verrijzer, C., Yokomori, K., Chen, J.-L., & Tjian, R. (1994) · Science · doi:10.1126/science.8178153 · Cited by 206 · see citing works
Capili, A. D., Schultz, D., Rauscher, F. J., & Borden, K. L. B. (2001) · The EMBO Journal · doi:10.1093/emboj/20.1.165 · Cited by 203 · see citing works
Multiple Mechanisms of Transcriptional Repression by YY1
Galvin, K. M., & Shi, Y. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.7.3723 · Cited by 183 · see citing works
BDNF Selectively Regulates GABA A Receptor Transcription by Activation of the JAK/STAT Pathway
Lund, I. V., Hu, Y., Raol, Y. H., Benham, R. S., Faris, R., Russek, S. J., & Brooks‐Kayal, A. R. (2008) · Science Signaling · doi:10.1126/scisignal.1162396 · Cited by 181 · see citing works
Self-citationYeast TAFII145 Functions as a Core Promoter Selectivity Factor, Not a General Coactivator
Shen, W.-C., & Green, M. R. (1997) · Cell · doi:10.1016/s0092-8674(00)80523-1 · Cited by 179 · see citing works
Raol, Y. H., Lund, I. V., Bandyopadhyay, S., Zhang, G., Roberts, D. S., Wolfe, J. H. N., Russek, S. J., & Brooks‐Kayal, A. R. (2006) · Journal of Neuroscience · doi:10.1523/jneurosci.3329-06.2006 · Cited by 176 · see citing works
Self-citationThe basal initiation machinery: beyond the general transcription factors
Sikorski, T. W., & Buratowski, S. (2009) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2009.03.006 · Cited by 176 · see citing works
Role of hPHF1 in H3K27 Methylation and Hox Gene Silencing
Cao, R., Wang, H., He, J., Erdjument‐Bromage, H., Tempst, P., & Zhang, Y. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.01589-07 · Cited by 173 · see citing works
The initiator directs the assembly of a transcription factor IID-dependent transcription complex.
Cárcamo, J. M., Buckbinder, L., & Reinberg, D. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.18.8052 · Cited by 169 · see citing works
Structure of the PHD Zinc Finger from Human Williams-Beuren Syndrome Transcription Factor
Pascual, J., Martinez‐Yamout, M. A., Dyson, H. J., & Wright, P. E. (2000) · Journal of Molecular Biology · doi:10.1006/jmbi.2000.4308 · Cited by 156 · see citing works
The Polycomb group — no longer an exclusive club?
Brock, H. W., & van Lohuizen, M. (2001) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(00)00176-3 · Cited by 149 · see citing works
GAL11P: A yeast mutation that potentiates the effect of weak GAL4-derived activators
Himmelfarb, H. J., Pearlberg, J., Last, D. H., & Ptashne, M. (1990) · Cell · doi:10.1016/0092-8674(90)90425-e · Cited by 147 · see citing works
Lonie, A. J., D’Andrea, R. J., Paro, R., & Saint, R. B. (1994) · Development · doi:10.1242/dev.120.9.2629 · Cited by 138 · see citing works
The Yin and Yang of YY1 in the nervous system
He, Y., & Casaccia‐Bonnefil, P. (2008) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2008.05486.x · Cited by 132 · see citing works
A 1-Megadalton ESC/E(Z) Complex from Drosophila That Contains Polycomblike and RPD3
Tie, F., Prasad-Sinha, J., Birve, A., Rasmuson-Lestander, Å., & Harte, P. J. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.9.3352-3362.2003 · Cited by 132 · see citing works
Probabilistic prediction and ranking of human protein-protein interactions
Scott, M. S., & Barton, G. J. (2007) · BMC Bioinformatics · doi:10.1186/1471-2105-8-239 · Cited by 132 · see citing works
Nucleosome Binding by the Bromodomain and PHD Finger of the Transcriptional Cofactor p300
Ragvin, A., Valvatne, H., Erdal, S., Årskog, V., Tufteland, K. R., Breen, K., Oyan, A. M., Eberharter, A., Gibson, T. J., Becker, P. B., et al. (2004) · Journal of Molecular Biology · doi:10.1016/j.jmb.2004.01.051 · Cited by 129 · see citing works
A Conserved Sequence Motif in Polycomb-Response Elements
Mihály, J., Mishra, R. K., & Karch, F. (1998) · Molecular Cell · doi:10.1016/s1097-2765(00)80107-0 · Cited by 121 · see citing works
Roberts, D. S., Raol, Y. H., Bandyopadhyay, S., Lund, I. V., Budreck, E. C., Passini, M. J., Wolfe, J. H. N., Brooks‐Kayal, A. R., & Russek, S. J. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0501434102 · Cited by 115 · see citing works
Self-citationPolycomblike PHD Fingers Mediate Conserved Interaction with Enhancer of Zeste Protein
O’Connell, S., Wang, L., Robert, S. S., et al. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m104294200 · Cited by 109 · see citing works
Choi, J. K., & Howe, L. J. (2009) · Biochemistry and Cell Biology · doi:10.1139/o08-112 · Cited by 104 · see citing works
The PHD Type Zinc Finger Is an Integral Part of the CBP Acetyltransferase Domain
Kalkhoven, E., Teunissen, H., Houweling, A., Verrijzer, C. P., & ZANTEMA, A. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.7.1961-1970.2002 · Cited by 104 · see citing works
Poux, S., Horard, B., Sigrist, C. J. A., & Pirrotta, V. (2002) · Development · doi:10.1242/dev.129.10.2483 · Cited by 103 · see citing works
Ma, W., & Barker, J. L. (1995) · Journal of Neuroscience · doi:10.1523/jneurosci.15-03-02547.1995 · Cited by 102 · see citing works
Weis, L., & Reinberg, D. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.6.2973 · Cited by 100 · see citing works
TAFII250-dependent transcription of cyclin A is directed by ATF activator proteinsPreprint
Wang, E. H., Zou, S., & Tjian, R. (1997) · Genes & Development · doi:10.1101/gad.11.20.2658 · Cited by 86 · see citing works
Hu, Y., Lund, I. V., Gravielle, M. C., Farb, D. H., Brooks‐Kayal, A. R., & Russek, S. J. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m705110200 · Cited by 81 · see citing works
Self-citationHerpes simplex virus 1 ICP0 co-localizes with a SUMO-specific protease
Bailey, D., & O’Hare, P. (2002) · Journal of General Virology · doi:10.1099/0022-1317-83-12-2951 · Cited by 77 · see citing works
Up‐regulation of NMDAR1 subunit gene expression in cortical neurons via a PKA‐dependent pathway
Lau, G. C., Saha, S., Faris, R., & Russek, S. J. (2004) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2003.02156.x · Cited by 72 · see citing works
Self-citationBaumann, M., Pontiller, J., & Ernst, W. (2010) · Molecular Biotechnology · doi:10.1007/s12033-010-9265-6 · Cited by 70 · see citing works
Martinez, E., Ge, H., Tao, Y., et al. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.11.6571 · Cited by 70 · see citing works
Zeste maintains repression ofUbxtransgenes: support for a new model of Polycomb repression
Hur, M., Laney, J. D., Jeon, S.-H., et al. (2002) · Development · doi:10.1242/dev.129.6.1339 · Cited by 62 · see citing works
Russek, S. J., Bandyopadhyay, S., & Farb, D. H. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.15.8600 · Cited by 45 · see citing works
Self-citationCoulson, M., Robert, S. S., Eyre, H. J., et al. (1998) · Genomics · doi:10.1006/geno.1997.5201 · Cited by 43 · see citing works
Structure-Function Analysis of TFII-I
Cheriyath, V., & Roy, A. L. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m008411200 · Cited by 36 · see citing works
H3K36me3 key to Polycomb-mediated gene silencing in lineage specification
Abed, J. A., & Jones, R. S. (2012) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2458 · Cited by 33 · see citing works
Yin Yang 1, a vertebrate Polycomb group gene, regulates antero-posterior neural patterning
Kwon, H.-J., & Chung, H. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(03)01071-4 · Cited by 32 · see citing works
Yoshitake, Y., Howard, T. L., Christian, J. L., & Hollenberg, S. M. (1999) · Developmental Biology · doi:10.1006/dbio.1999.9473 · Cited by 23 · see citing works
Korhonen, P., Huotari, V., Soininen, H., et al. (1997) · Molecular Brain Research · doi:10.1016/s0169-328x(97)00310-0 · Cited by 23 · see citing works
Barnett, M. W., Seville, R. A., Nijjar, S. S., et al. (2001) · Mechanisms of Development · doi:10.1016/s0925-4773(01)00304-5 · Cited by 20 · see citing works
Xenopus Polycomblike 2 (XPcl2) controls anterior to posterior patterning of the neural tissue
Kitaguchi, T., Nakata, K., Nagai, T., Aruga, J., & Mikoshiba, K. (2001) · Development Genes and Evolution · doi:10.1007/s004270100157 · Cited by 16 · see citing works
Sectorial gene repression in the control of development
Zuckerkandl, E. (1999) · Gene · doi:10.1016/s0378-1119(99)00293-0 · Cited by 8 · see citing works
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Mozzi, A., Forni, D., Cagliani, R., Pozzoli, U., Clerici, M., & Sironi, M. (2017) · Scientific Reports · doi:10.1038/s41598-017-06440-4 · Cited by 19 · see citing works
- Authors
- Saha, S., Hu, Y., Martin, S. C., Bandyopadhyay, S., Russek, S. J., & Farb, D. H.
- Affiliations
- Boston University (US)
- Corresponding author
- Russek, Shelley J
- Venue
- BMC Pharmacology and Toxicology
- Published
- 2013-07-23
- Volume
- 14
- Issue
- 1
- Pages
- 37-37
- ISSN
- 2050-6511 (ISSN-L)
- PMC
- PMC3734045
- OpenAlex
- W1990026602
- Cited by
- 9
- References
- 83
- Citations by year
- 2024: 1 · 2022: 2 · 2020: 1 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 1
- FWCI
- 0.30
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- BMC Pharmacology and Toxicology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- School of Medicine, Boston University
- National Institutes of Health
- National Institute on Alcohol Abuse and Alcoholism (R01AA011697, R01 AA11697-09)
- National Institute of Neurological Disorders and Stroke (R01NS050393, R01 NS050393-010A)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- GABAA receptor, GABRB1, transcriptional regulation, plant homeodomain, SUZ12, polycomb repressive complex 2, H3K27 methylation, cortical neurons, GABA signaling
- MeSH terms
- Animals, Cells, Cultured, Chlorocebus aethiops, DNA-Binding Proteins, gamma-Aminobutyric Acid, Hippocampus, Humans, Male, Neurons, Receptors, GABA-A, Transcription Factors, Transcription, Genetic, Rats, Sprague-Dawley, COS Cells, Neocortex, Two-Hybrid System Techniques, Protein Subunits, Rats, Polycomb-Group Proteins
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