Cited by 55Dialysis and Renal Disease ManagementTGF-β signaling in diseasesGenomics, phytochemicals, and oxidative stress

Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5

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Zhao, Junyi, Shi, Jun, Shan, Yun, Yu, Manshu, Zhu, Xiaolin, Zhu, Yilin, Liu, Li, Sheng, Meixiao·2020·Cellular & Molecular Biology Letters

Abstract

Background: Peritoneal fibrosis (PF) is a frequent complication caused by peritoneal dialysis (PD). Peritoneal mesothelial cells (PMCs), the first barrier of the peritoneum, play an important role in maintaining structure and function in the peritoneum during PD. Mesothelial-mesenchymal transition (MMT) and oxidative stress of PMCs are two key processes of PF. Purpose: To elucidate the efficacy and possible mechanism of asiaticoside inhibition of MMT and ROS generation in TGF-β1-induced PF in human peritoneal mesothelial cells (HPMCs). Methods: MMT and ROS generation of HPMCs were induced by TGF-β1. To explain the anti-MMT and antioxidant role of asiaticoside, varied doses of asiaticoside, oxygen radical scavenger (NAC), TGF-β receptor kinase inhibitor (LY2109761) and Nrf2 inhibitor (ML385) were used separately. Immunoblots were used to detect the expression of signaling associated proteins. DCFH-DA was used to detect the generation of ROS. Transwell migration assay and wound healing assay were used to verify the capacity of asiaticoside to inhibit MMT. Immunofluorescence assay was performed to observe the subcellular translocation of Nrf2 and expression of HO-1. Results: Asiaticoside inhibited TGF-β1-induced MMT and suppressed Smad signaling in a dose-dependent manner. Migration and invasion activities of HPMCs were decreased by asiaticoside. Asiaticoside decreased TGF-β1-induced ROS, especially in a high dose (150 μM) for 6 h. Furthermore, ML385 partly abolished the inhibitory effect of asiaticoside on MMT, ROS and p-Smad2/3. Conclusions: Asiaticoside inhibited the TGF-β1-induced MMT and ROS via Nrf2 activation, thus protecting the peritoneal membrane and preventing PF.

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  • Antioxidant and cytoprotective benefits from C. asiatica are mediated through direct ROS scavenging and upregulation of endogenous antioxidant enzymes such as SOD and catalase.

    “Antioxidant and cytoprotective benefits from C. asiatica are mediated through direct ROS scavenging and upregulation of endogenous antioxidant enzymes such as superoxide dismutase (SOD) and catalase (Zhao et al. 2020; Mohapatra et al. 2021; Jiang et al. 2022).”

    From Centella asiatica (L.) Urb. in skin health and cosmeceuticals: mechanisms, clinical evidence, and advanced delivery systems (2025) · cites this paper as Zhao et al. 2020

    The Results report that asiaticoside reduced TGF-β1-induced ROS in human peritoneal mesothelial cells and increased the antioxidant enzyme HO-1 through Nrf2 activation. They do not establish direct ROS scavenging by asiaticoside or report upregulation of SOD or catalase; the paper identifies NAC, not asiaticoside, as an oxygen radical scavenger. (LLM, conf=0.89)

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Authors
Zhao, J., Shi, J., Shan, Y., Yu, M., Zhu, X., Zhu, Y., Liu, L., & Sheng, M.
Affiliations
Nanjing University of Chinese Medicine (CN)
Corresponding author
Sheng, Meixiao
Venue
Cellular & Molecular Biology Letters
Published
2020-05-29
Volume
25
Issue
1
Pages
33-33
ISSN
  • 1425-8153 (ISSN-L)
  • 1689-1392
OpenAlex
W3028774629
Cited by
55
References
38
Citations by year
2026: 11 · 2025: 11 · 2024: 8 · 2023: 11 · 2022: 10 · 2021: 3 · 2020: 1
FWCI
3.93
Type
journal article
Language
en
Open access
Locations
  • Cellular & Molecular Biology Letters (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (81774253, 81904121)
  • Natural Science Foundation of Jiangsu Province (BK20171514)
  • Leading Talent Project in Jiangsu Province (SLJ0205)
  • Summit Scholar Project in Jiangsu Province Hospital of Chinese Medicine (y2018rc08)
Topics
  • Health Sciences › Medicine › Nephrology › Dialysis and Renal Disease Management
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › TGF-β signaling in diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
Keywords
asiaticoside, peritoneal fibrosis, peritoneal dialysis, mesothelial-to-mesenchymal transition, oxidative stress, reactive oxygen species, Nrf2/HO-1 pathway, TGF-β1, Smad signaling, peritoneal mesothelial cells, cell migration and invasion, Nrf2 inhibitors
MeSH terms
Cell Line, Epithelial Cells, Humans, Triterpenes, Signal Transduction, Apoptosis, Reactive Oxygen Species, Oxidative Stress, NF-E2-Related Factor 2, Heme Oxygenase-1, Smad Proteins, Transforming Growth Factor beta1, Peritoneal Fibrosis, Epithelial-Mesenchymal Transition

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