Micro/nano multilayered scaffolds of PLGA and collagen by alternately electrospinning for bone tissue engineering
Open accessAbstract
The dual extrusion electrospinning technique was used to fabricate multilayered 3D scaffolds by stacking microfibrous meshes of poly(lactic acid-co-glycolic acid) (PLGA) in alternate fashion to micro/nano mixed fibrous meshes of PLGA and collagen. To fabricate the multilayered scaffold, 35 wt% solution of PLGA in THF-DMF binary solvent (3:1) and 5 wt% solution of collagen in hexafluoroisopropanol (HFIP) with and without hydroxyapatite nanorods (nHA) were used. The dual and individual electrospinning of PLGA and collagen were carried out at flow rates of 1.0 and 0.5 mL/h, respectively, at an applied voltage of 20 kV. The density of collagen fibers in multilayered scaffolds has controlled the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells. The homogeneous dispersion of glutamic acid-modified hydroxyapatite nanorods (nHA-GA) in collagen solution has improved the osteogenic properties of fabricated multilayered scaffolds. The fabricated multilayered scaffolds were characterized using FT-IR, X-ray photoelectron spectroscopy, and transmission electron microscopy (TEM). The scanning electron microscopy (FE-SEM) was used to evaluate the adhesion and spreads of MC3T3-E1 cells on multilayered scaffolds. The activity of MC3T3-E1 cells on the multilayered scaffolds was evaluated by applying MTT, alkaline phosphatase, Alizarin Red, von Kossa, and cytoskeleton F-actin assaying protocols. The micro/nano fibrous PLGA-Col-HA scaffolds were found to be highly bioactive in comparison to pristine microfibrous PLGA and micro/nano mixed fibrous PLGA and Col scaffolds.
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The PLGA/collagen/nano-HA electrospun scaffold showed controlled adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.
“The developed scaffold showed controlled adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells.[106]”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [106]
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Cited by
Sorted by citations
Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration.
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Self-citation
- Authors
- Kwak, S., Haider, A., Gupta, K. C., Kim, S., & Kang, I.-K.
- Affiliations
- Kyungpook National University (KR)
- Indian Institute of Technology Roorkee (IN)
- Yeungnam University (KR)
- Corresponding author
- Gupta, Kailash Chandra
- Kang, Inn-Kyu
- Venue
- Nanoscale Research Letters
- Published
- 2016-07-04
- Volume
- 11
- Issue
- 1
- Pages
- 323-323
- ISSN
- 1556-276X (ISSN-L)
- 1931-7573
- PMC
- PMC4932007
- OpenAlex
- W2466974886
- Cited by
- 99
- References
- 75
- Citations by year
- 2026: 8 · 2025: 11 · 2024: 5 · 2023: 12 · 2022: 9 · 2021: 19 · 2020: 6 · 2019: 12 · 2018: 9 · 2017: 7 · 2016: 1
- FWCI
- 4.66
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nanoscale Research Letters (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- kyungpook National University Research Fund
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Keywords
- multilayered scaffolds, PLGA, collagen, bone tissue engineering, hydroxyapatite nanorods, osteogenic differentiation, MC3T3-E1 cells, bioactivity (biomaterials), cell adhesion
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