Cited by 99Bone Tissue Engineering MaterialsElectrospun Nanofibers in Biomedical ApplicationsSilk-based biomaterials and applications

Micro/nano multilayered scaffolds of PLGA and collagen by alternately electrospinning for bone tissue engineering

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Kwak, Sanghwa, Haider, Adnan, Gupta, Kailash Chandra, Kim, Sukyoung, Kang, Inn-Kyu·2016·Nanoscale Research Letters

Abstract

The dual extrusion electrospinning technique was used to fabricate multilayered 3D scaffolds by stacking microfibrous meshes of poly(lactic acid-co-glycolic acid) (PLGA) in alternate fashion to micro/nano mixed fibrous meshes of PLGA and collagen. To fabricate the multilayered scaffold, 35 wt% solution of PLGA in THF-DMF binary solvent (3:1) and 5 wt% solution of collagen in hexafluoroisopropanol (HFIP) with and without hydroxyapatite nanorods (nHA) were used. The dual and individual electrospinning of PLGA and collagen were carried out at flow rates of 1.0 and 0.5 mL/h, respectively, at an applied voltage of 20 kV. The density of collagen fibers in multilayered scaffolds has controlled the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells. The homogeneous dispersion of glutamic acid-modified hydroxyapatite nanorods (nHA-GA) in collagen solution has improved the osteogenic properties of fabricated multilayered scaffolds. The fabricated multilayered scaffolds were characterized using FT-IR, X-ray photoelectron spectroscopy, and transmission electron microscopy (TEM). The scanning electron microscopy (FE-SEM) was used to evaluate the adhesion and spreads of MC3T3-E1 cells on multilayered scaffolds. The activity of MC3T3-E1 cells on the multilayered scaffolds was evaluated by applying MTT, alkaline phosphatase, Alizarin Red, von Kossa, and cytoskeleton F-actin assaying protocols. The micro/nano fibrous PLGA-Col-HA scaffolds were found to be highly bioactive in comparison to pristine microfibrous PLGA and micro/nano mixed fibrous PLGA and Col scaffolds.

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  1. [1]Smith LA, Beck JA, Ma PX (2006) Nanofibrous scaffolds and their biological effect. In: Kumar C (ed) Nanotechnologies for the life sciences. Tissue cell and organ engineering, Wiley-VCH Verlag GmbH & Co., Weinheim, p 188-215
  2. [2]Ikada Y (2011) Scope of tissue engineering. In: Ikada Y (ed) Tissue Engineering: Fundamental and Application, vol 8. Academic Press, USA, p 1-89

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Authors
Kwak, S., Haider, A., Gupta, K. C., Kim, S., & Kang, I.-K.
Affiliations
  • Kyungpook National University (KR)
  • Indian Institute of Technology Roorkee (IN)
  • Yeungnam University (KR)
Corresponding author
  • Gupta, Kailash Chandra
  • Kang, Inn-Kyu
Venue
Nanoscale Research Letters
Published
2016-07-04
Volume
11
Issue
1
Pages
323-323
ISSN
  • 1556-276X (ISSN-L)
  • 1931-7573
OpenAlex
W2466974886
Cited by
99
References
75
Citations by year
2026: 8 · 2025: 11 · 2024: 5 · 2023: 12 · 2022: 9 · 2021: 19 · 2020: 6 · 2019: 12 · 2018: 9 · 2017: 7 · 2016: 1
FWCI
4.66
Type
journal article
Language
en
Open access
Locations
  • Nanoscale Research Letters (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
kyungpook National University Research Fund
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
  • Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
Keywords
multilayered scaffolds, PLGA, collagen, bone tissue engineering, hydroxyapatite nanorods, osteogenic differentiation, MC3T3-E1 cells, bioactivity (biomaterials), cell adhesion

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