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Cited by 20Infective Endocarditis Diagnosis and ManagementProbiotics and Fermented FoodsMicrobial infections and disease research

Characterization of a bacterial strain Lactobacillus paracasei LP10266 recovered from an endocarditis patient in Shandong, China

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Tang, Qi, Hao, Yingying, Wang, Lu, Lu, Chao, Li, Ming, Si, Zaifeng, Wu, Xiaoben, Lu, Zhiming·2021·BMC Microbiology
DOI 10.1186/s12866-021-02253-8PMC PMC8210379
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Abstract

Background: Lactobacilli are often recognized as beneficial partners in human microbial environments. However, lactobacilli also cause diseases in human, e.g. infective endocarditis (IE), septicaemia, rheumatic vascular disease, and dental caries. Therefore, the identification of potential pathogenic traits associated with lactobacilli will facilitate the prevention and treatment of the diseases caused by lactobacilli. Herein, we investigated the genomic traits and pathogenic potential of a novel bacterial strain Lactobacillus paracasei LP10266 which has caused a case of IE. We isolated L. paracasei LP10266 from an IE patient’s blood to perform high-throughput sequencing and compared the genome of strain LP10266 with those of closely related lactobacilli to determine genes associated with its infectivity. We performed the antimicrobial susceptibility testing on strain LP10266. We assessed its virulence by mouse lethality and serum bactericidal assays as well as its serum complement- and platelet-activating ability. The biofilm formation and adherence of strain LP10266 were also studied. Results: Phylogenetic analysis revealed that strain LP10266 was allied with L. casei and L. paracasei. Genomic studies revealed two spaCBA pilus clusters and one novel exopolysaccharides (EPS) cluster in strain LP10266, which was sensitive to ampicillin, penicillin, levofloxacin, and imipenem, but resistant to cefuroxime, cefazolin, cefotaxime, meropenem, and vancomycin. Strain LP10266 was nonfatal and sensitive to serum, capable of activating complement 3a and terminal complement complex C5b-9 (TCC). Strain LP10266 could not induce platelet aggregation but displayed a stronger biofilm formation ability and adherence to human vascular endothelial cells (HUVECs) compared to the standard control strain L. paracasei ATCC25302. Conclusion: The genome of a novel bacterial strain L. paracasei LP10266 was sequenced. Our results based on various types of assays consistently revealed that L. paracasei LP10266 was a potential pathogen to patients with a history of cardiac disease and inguinal hernia repair. Strain LP10266 showed strong biofilm formation ability and adherence, enhancing the awareness of L. paracasei infections.

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  • Our review revealed 49 case reports of LB without any history of probiotic use.

    “Our review also revealed 49 case reports of LB without any history of probiotic use (Table 1) [24,26,27,33,35,45,46,47,48,49,50,51].”

    From Lactobacillus Bacteremia and Probiotics: A Review (2023) · cites this paper as [49]

    The Results (Case report; page not provided) describe one patient with Lactobacillus paracasei isolated from blood, so the paper can serve as an example of a reported case. The case report does not state whether that patient used probiotics. It also does not report the citing authors’ review count of 49 cases. (LLM, conf=0.78)

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Cited by

Sorted by citations

Authors
Tang, Q., Hao, Y., Wang, L., Lu, C., Li, M., Si, Z., Wu, X., & Lu, Z.
Affiliations
  • Shandong University (CN)
  • Shandong Provincial Hospital (CN)
Corresponding author
  • Wu, Xiaoben
  • Lu, Zhiming
ORCID
Venue
BMC Microbiology
Publisher
Springer Science and Business Media LLC
Published
2021-06-17
Volume
21
Issue
1
Pages
183-183
ISSN
1471-2180 (ISSN-L)
DOI
10.1186/s12866-021-02253-8
PMC
PMC8210379
OpenAlex
W3169534705
Cited by
20
References
5
Citations by year
2025: 6 · 2024: 2 · 2023: 4 · 2022: 7 · 2021: 1
FWCI
2.52
Type
journal article
Language
en
Open access
Licence
Locations
  • BMC Microbiology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Natural Science Foundation of China (81902119)
Topics
  • Health Sciences › Medicine › Epidemiology › Infective Endocarditis Diagnosis and Management
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
Lactobacillus paracasei, infective endocarditis, bacterial virulence, pathogenic traits, exopolysaccharide biosynthesis, antimicrobial resistance, complement activation, platelet activation, biofilm formation, bacterial adhesion, endothelial cells, mouse lethality, serum bactericidal activity, Lactobacillus casei
MeSH terms
Lacticaseibacillus paracasei, China, Endocarditis, Bacterial, Humans, Male, Middle Aged, Phylogeny, Genome, Bacterial, Biofilms

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