Comparative genomic analysis revealed genetic divergence between Bifidobacterium catenulatum subspecies present in infant versus adult guts
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Background: The two subspecies of Bifidobacterium catenulatum , B. catenulatum subsp. kashiwanohense and B. catenulatum subsp. catenulatum , are usually from the infant and adult gut, respectively. However, the genomic analysis of their functional difference and genetic divergence has been rare. Here, 16 B. catenulatum strains, including 2 newly sequenced strains, were analysed through comparative genomics. Results: A phylogenetic tree based on 785 core genes indicated that the two subspecies of B. catenulatum were significantly separated. The comparison of genomic characteristics revealed that the two subspecies had significantly different genomic sizes ( p < 0.05) but similar GC contents. The functional comparison revealed the most significant difference in genes of carbohydrate utilisation. Carbohydrate-active enzymes (CAZyme) present two clustering patterns in B. catenulatum. The B. catenulatum subsp. kashiwanohense specially including the glycoside hydrolases 95 (GH95) and carbohydrate-binding modules 51 (CBM51) families involved in the metabolism of human milk oligosaccharides (HMO) common in infants, also, the corresponding fucosylated HMO gene clusters were detected. Meanwhile, B. catenulatum subsp. catenulatum rich in GH3 may metabolise more plant-derived glycan in the adult intestine. Conclusions: These findings provide genomic evidence of carbohydrate utilisation bias, which may be a key cause of the genetic divergence of two B. catenulatum subspecies.
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Previous studies of B. bifidum and other HBS have focused more on descriptive aspects.
“adds to the results of previous studies, which have focused more on descriptive aspects in B. bifidum [29,104] and other HBS [105-107]”
From Genome-scale insights into the metabolic landscape and evolutionary development of Bifidobacterium bifidum (2026) · cites this paper as [105]
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Self-citationSelleri, E., Longhi, G., Tarracchini, C., Carolis, E. D., Petraro, S., Mancabelli, L., Milani, C., Turroni, F., Ventura, M., & Lugli, G. A. (2026) · Microbiome Research Reports · doi:10.20517/mrr.2026.19
- Authors
- Liu, J., Li, W., Yao, C., Yu, J., & Zhang, H.
- Affiliations
- Inner Mongolia Agricultural University (CN)
- Corresponding author
- Zhang, Heping
- Venue
- BMC Microbiology
- Published
- 2022-06-16
- Volume
- 22
- Issue
- 1
- Pages
- 158-158
- ISSN
- 1471-2180 (ISSN-L)
- PMC
- PMC9202165
- OpenAlex
- W4283032507
- Cited by
- 22
- References
- 61
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 8 · 2023: 3 · 2022: 2
- FWCI
- 1.39
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- BMC Microbiology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- Bifidobacterium catenulatum, genetic divergence, infant gut microbiota, carbohydrate-active enzymes, human milk oligosaccharides, GH3 cells, core genome phylogeny, strain genomics
- MeSH terms
- Bifidobacterium, Carbohydrates, Humans, Infant, Milk, Human, Oligosaccharides, Phylogeny, Genomics
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