The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Antisense knockdown of pyruvate dehydrogenase kinase promotes the neutral lipid accumulation in the diatom Phaeodactylum tricornutum
Open accessAbstract
BACKGROUND: Microalgae have been an emerging biofuel resource; however, the germplasm improvement has been slow due to the lack of molecular tools. Pyruvate dehydrogenase kinase (PDK) deactivates the pyruvate dehydrogenase complex (PDC) which catalyzes the oxidative decarboxylation of pyruvate. Acetyl-CoA production via PDC is important in plant tissues that are active in fatty acid synthesis. RESULTS: A 1261-bp cDNA of a putative PDK gene (PtPDK) was cloned from a diatom Phaeodactylum tricornutum, and PtPDK antisense knockdown transgenic diatoms were generated. Both PtPDK transcript abundance and enzyme activity were reduced significantly due to antisense knockdown of PtPDK. Neutral lipid content of transgenic diatom cells increased up to 82% as determined by Nile red staining, and fatty acid composition was not altered. Transgenic cells showed slightly lower growth rate but similar cell size with the wild type, hence retaining similar biomass productivity. CONCLUSIONS: This work first obtained a successful engineered diatom regulating a key gene involved in lipid metabolism. Our findings also provide powerful indications in enhancing microalgal lipid production by metabolic engineering for biofuel industry.
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“The exceptionally high content of total lipids was noted in microalgae Prymnesium parvum (65.79%) [99], Vischeria vischeri (65.16%) [100], Nannochloropsis granulata CCMP525 (60.35%) [101], Chlorella vulgaris (67.1%) [99], Tetradesmus deserticola JNU19 (62.4%) [102].”
From Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae (2023) · cites this paper as [101]
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Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
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MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
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“Phylogenetic tree of protein clusters from various species was constructed by neighborjoining (NJ) method using software MEGA 5 [20].”
MethodMicroalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
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“Microalgae have become an emerging biofuel resource [3].”
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The Genome of the Diatom Thalassiosira Pseudonana : Ecology, Evolution, and Metabolism
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“Particularly, a range of novel metabolic pathways have been found in diatoms which were presumably acquired during their evolution [1], so they are of major interest for the discovery of novel biological processes which are not present in other more intensively studied model organisms [2].”
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“pseudonana to improve lipid content by knockdown of a lipase gene [12].”
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BackgroundInfluentialYang, Z.-K., Niu, Y., Ma, Y.-H., Xue, J., Zhang, M.-H., Yang, W.-D., Liu, J.-S., Lu, S.-H., Guan, Y., & Li, H. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-67 · Cited by 366 · cited 2×
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BackgroundMethodSelf-citationMolecular toolbox for studying diatom biology in Phaeodactylum tricornutum
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“Particularly, a range of novel metabolic pathways have been found in diatoms which were presumably acquired during their evolution [1], so they are of major interest for the discovery of novel biological processes which are not present in other more intensively studied model organisms [2].”
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The Green Biofuel Conundrum: Technological Advances Versus Economic and Environmental Implications
Sanath, S., Roman, S., Lorenzo, S., & Riccardo, S. (2021) · ACTA SCIENTIFIC MICROBIOLOGY · doi:10.31080/asmi.2021.04.0882
Metabolic engineering and molecular biotechnology of microalgae for fuel production
Fang, S. (2019) · Elsevier eBooks · doi:10.1016/b978-0-444-64192-2.00005-6
Miller, S. M., Herdean, A., Gupta, V., Signal, B., Abbriano, R. M., Ralph, P. J., & Pernice, M. (2023) · Research Square · doi:10.21203/rs.3.rs-3012279/v1
Atikij, T., & Waditee-Sirisattha, R. (2018) · doi:10.58837/chula.the.2018.369
The life cycle of fatty acids in oleaginous stramenopiles: from synthesis to maturation and beyond
Sérès, Y., Serbutoviez-Verville, C., Maréchal, É., & Amato, A. (2026) · BMC Plant Biology · doi:10.1186/s12870-026-08803-4
Mohan, K., Sreenikethanam, A., Raj, S., Mondru, N., & Bajhaiya, A. K. (2026) · Frontiers in Fuels · doi:10.3389/ffuel.2026.1860328
Metadata
- Authors
- Ma, Y.-H., Wang, X., Niu, Y.-F., Yang, Z.-K., Zhang, M.-H., Wang, Z.-M., Yang, W.-D., Liu, J.-S., & Li, H.-Y.
- Affiliations
- Jinan University (CN)
- Venue
- Microbial Cell Factories
- Published
- 2014-08-09
- Volume
- 13
- Issue
- 1
- Pages
- 100-100
- ISSN
- 1475-2859 (ISSN-L)
- PMC
- PMC4251698
- OpenAlex
- W2108487405
- Cited by
- 106
- References
- 30
- Citations by year
- 2026: 2 · 2025: 10 · 2024: 2 · 2023: 10 · 2022: 8 · 2021: 12 · 2020: 9 · 2019: 19 · 2018: 10 · 2017: 11 · 2016: 9 · 2015: 4
- FWCI
- 2.64
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Microbial Cell Factories (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Institutional Repository of Guangzhou Institute of Energy Research, Chinese Academy of Sciences (submittedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- pyruvate dehydrogenase kinase, Phaeodactylum tricornutum, diatoms, neutral lipid accumulation, pyruvate dehydrogenase complex, antisense knockdown, microalgal lipid production, metabolic engineering, biofuels, fatty acid composition, acetyl-CoA
- MeSH terms
- Pyruvate Dehydrogenase Acetyl-Transferring Kinase, RNA, Antisense, Protein Serine-Threonine Kinases, Diatoms, Lipid Metabolism, Gene Knockdown Techniques, Microalgae, Metabolic Engineering
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