The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Antisense knockdown of pyruvate dehydrogenase kinase promotes the neutral lipid accumulation in the diatom Phaeodactylum tricornutum
Open accessAbstract
BACKGROUND: Microalgae have been an emerging biofuel resource; however, the germplasm improvement has been slow due to the lack of molecular tools. Pyruvate dehydrogenase kinase (PDK) deactivates the pyruvate dehydrogenase complex (PDC) which catalyzes the oxidative decarboxylation of pyruvate. Acetyl-CoA production via PDC is important in plant tissues that are active in fatty acid synthesis. RESULTS: A 1261-bp cDNA of a putative PDK gene (PtPDK) was cloned from a diatom Phaeodactylum tricornutum, and PtPDK antisense knockdown transgenic diatoms were generated. Both PtPDK transcript abundance and enzyme activity were reduced significantly due to antisense knockdown of PtPDK. Neutral lipid content of transgenic diatom cells increased up to 82% as determined by Nile red staining, and fatty acid composition was not altered. Transgenic cells showed slightly lower growth rate but similar cell size with the wild type, hence retaining similar biomass productivity. CONCLUSIONS: This work first obtained a successful engineered diatom regulating a key gene involved in lipid metabolism. Our findings also provide powerful indications in enhancing microalgal lipid production by metabolic engineering for biofuel industry.
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“The exceptionally high content of total lipids was noted in microalgae Prymnesium parvum (65.79%) [99], Vischeria vischeri (65.16%) [100], Nannochloropsis granulata CCMP525 (60.35%) [101], Chlorella vulgaris (67.1%) [99], Tetradesmus deserticola JNU19 (62.4%) [102].”
From Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae (2023) · cites this paper as [101]
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Citations
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MethodMOLECULAR GENETIC MANIPULATION OF THE DIATOM THALASSIOSIRA PSEUDONANA (BACILLARIOPHYCEAE)1
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“The mitochondrial PDC, unlike its plastid counterpart, has an associated pyruvate dehydrogenase kinase (PDK) [7].”
BackgroundCharacteristics of the Gene That Encodes Acetyl‐CoA Carboxylase in the Diatom Cyclotella crypticaa
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“Flow cytometry is used as a rapid approach for qualitative and quantitative analysis of individual cells which can detect a variety of biochemical reactions accurately in cells [27], especially analysis of large number of individual cells by fluorescence staining [28].”
MethodThelen, J. J., Miernyk, J. A., & Randall, D. D. (2000) · Biochemical Journal · doi:10.1042/bj3490195 · Cited by 36
Igamberdiev, A. U., Lernmark, U., & Gardeström, P. (2014) · Mitochondrion · doi:10.1016/j.mito.2014.04.006 · Cited by 29 · cited 1×
“The mitochondrial pyruvate dehydrogenase complex (PDC) catalyzes the irreversible oxidative decarboxylation of pyruvate to acetyl-CoA, thus directing the carbon flow into TCA cycle for yielding NADH to satisfy the energy demands of cells [6].”
BackgroundCloning and functions analysis of a pyruvate dehydrogenase kinase in Brassica napus
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“Overexpression of Brassica napus PDK in Arabidopsis repressed the PDC activity, leading to a decrease in seed oil content [10].”
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Background
Cited by
Sorted by citations
The lipid biochemistry of eukaryotic algae
Li-Beisson, Y., Thelen, J. J., Fedosejevs, E., & Harwood, J. L. (2019) · Progress in Lipid Research · doi:10.1016/j.plipres.2019.01.003 · Cited by 453
“Activation of the pyruvate dehydrogenase complex (PDC) protein while silencing the pyruvate dehydrogenase kinase (PDK) has boosted acetyl-CoA production therefore neutral lipid content in Phaeodactylum tricornutum [121].”
BackgroundMicroalgae for high-value products: A way towards green nutraceutical and pharmaceutical compounds
Mehariya, S., Goswami, R. K., Karthikeysan, O. P., & Verma, P. (2021) · Chemosphere · doi:10.1016/j.chemosphere.2021.130553 · Cited by 303
Biofuel production from microalgae: a review
Peng, L., Fu, D., Chu, H., Wang, Z., & Qi, H. (2020) · Environmental Chemistry Letters · doi:10.1007/s10311-019-00939-0 · Cited by 227
Self-citationCurrent trends to comprehend lipid metabolism in diatoms
Zulu, N. N., Zienkiewicz, K., Vollheyde, K., & Feussner, I. (2018) · Progress in Lipid Research · doi:10.1016/j.plipres.2018.03.001 · Cited by 226
Storage of starch and lipids in microalgae: Biosynthesis and manipulation by nutrients
Ran, W., Wang, H., Liu, Y., Qi, M., Xiang, Q., Yao, C., Zhang, Y., & Lan, X. (2019) · Bioresource Technology · doi:10.1016/j.biortech.2019.121894 · Cited by 224
Metadata
- Authors
- Ma, Y.-H., Wang, X., Niu, Y.-F., Yang, Z.-K., Zhang, M.-H., Wang, Z.-M., Yang, W.-D., Liu, J.-S., & Li, H.-Y.
- Affiliations
- Jinan University (CN)
- Venue
- Microbial Cell Factories
- Published
- 2014-08-09
- Volume
- 13
- Issue
- 1
- Pages
- 100-100
- ISSN
- 1475-2859 (ISSN-L)
- PMC
- PMC4251698
- OpenAlex
- W2108487405
- Cited by
- 106
- References
- 30
- Citations by year
- 2026: 2 · 2025: 10 · 2024: 2 · 2023: 10 · 2022: 8 · 2021: 12 · 2020: 9 · 2019: 19 · 2018: 10 · 2017: 11 · 2016: 9 · 2015: 4
- FWCI
- 2.64
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Microbial Cell Factories (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Institutional Repository of Guangzhou Institute of Energy Research, Chinese Academy of Sciences (submittedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- pyruvate dehydrogenase kinase, Phaeodactylum tricornutum, diatoms, neutral lipid accumulation, pyruvate dehydrogenase complex, antisense knockdown, microalgal lipid production, metabolic engineering, biofuels, fatty acid composition, acetyl-CoA
- MeSH terms
- Pyruvate Dehydrogenase Acetyl-Transferring Kinase, RNA, Antisense, Protein Serine-Threonine Kinases, Diatoms, Lipid Metabolism, Gene Knockdown Techniques, Microalgae, Metabolic Engineering
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