Cited by 152Nanoparticles: synthesis and applicationsOccupational and environmental lung diseasesImmunotoxicology and immune responses

The DOI matches its Crossref record; 119 of the 120 references it cites were found in a registry or an index, and 1 are not checked yet.

An in vitro alveolar macrophage assay for predicting the short-term inhalation toxicity of nanomaterials

Open access

Wiemann, Martin, Vennemann, Antje, Sauer, Ursula G., Wiench, Karin, Ma-Hock, Lan, Landsiedel, Robert·2016·Journal of Nanobiotechnology

DOI 10.1186/s12951-016-0164-2PMC PMC4779246

Open this paper in CoChat — free account Open PDF

Abstract

BACKGROUND: Most in vitro studies investigating nanomaterial pulmonary toxicity poorly correlate to in vivo inhalation studies. Alveolar macrophages (AMs) play an outstanding role during inhalation exposure since they effectively clear the alveoli from particles. This study addresses the applicability of an in vitro alveolar macrophage assay to distinguish biologically active from passive nanomaterials. METHODS: Rat NR8383 alveolar macrophages were exposed to 18 inorganic nanomaterials, covering AlOOH, BaSO4, CeO2, Fe2O3, TiO2, ZrO2, and ZnO NMs, amorphous SiO2 and graphite nanoplatelets, and two nanosized organic pigments. ZrO2 and amorphous SiO2 were tested without and with surface functionalization. Non-nanosized quartz DQ12 and corundum were used as positive and negative controls, respectively. The test materials were incubated with the cells in protein-free culture medium. Lactate dehydrogenase, glucuronidase, and tumour necrosis factor alpha were assessed after 16 h. In parallel, H2O2 was assessed after 1.5 h. Using the no-observed-adverse-effect concentrations (NOAECs) from available rat short-term inhalation studies (STIS), the test materials were categorized as active (NOAEC < 10 mg/m(3)) or passive. RESULTS: In vitro data reflected the STIS categorization if a particle surface area-based threshold of <6000 mm(2)/mL was used to determine the biological relevance of the lowest observed significant in vitro effects. Significant effects that were recorded above this threshold were assessed as resulting from test material-unspecific cellular 'overload'. Test materials were assessed as active if ≥2 of the 4 in vitro parameters undercut this threshold. They were assessed as passive if 0 or 1 parameter was altered. An overall assay accuracy of 95 % was achieved. CONCLUSIONS: The in vitro NR8383 alveolar macrophage assay allows distinguishing active from passive nanomaterials. Thereby, it allows determining whether in vivo short-term inhalation testing is necessary for hazard assessment. Results may also be used to group nanomaterials by biological activity. Further work should aim at validating the assay.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1186/s12951-016-0164-2

Cites

119 of 120 references verified0 citation errors · 0 not checkable

Statistics

Fig. 1 Test material sedimentation and uptake by NR8383 rat alveolar macrophages. A Corundum; B Quartz DQ12; C TiO2 NM-105; D CeO2 NM-212; E SiO2.naked; F BaSO4 NM-220; G DPP Orange N; H Graphite nanoplatelets. Phase contrast micrographs show settled particles at the bottom of cell-free wells in 96-well plates (a1–h1 on the left side of the individual images) and corresponding particle-laden NR8383 cells at the end of the 16-h incubation period with the same particle concentration a2–h2 on the right side of the individual images). Note that the uptake of particles (loaded with 90 μg/mL) appears complete as viewed by light microscopy, except for SiO2.naked (loaded with 22.5 μg/mL), where settled particles are hardly visibleCC BY · PubMed Central

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Nanotoxicology: An Emerging Discipline Evolving from Studies of Ultrafine Particles
Oberdörster, G., Oberdörster, E., & Oberdörster, J. (2005) · Environmental Health Perspectives · doi:10.1289/ehp.7339 · Cited by 7,891
Understanding biophysicochemical interactions at the nano–bio interface
Nel, A. E., Mädler, L., Velegol, D., Xia, T., Hoek, E. M. V., Somasundaran, P., Klaessig, F., Castranova, V., & Thompson, M. (2009) · Nature Materials · doi:10.1038/nmat2442 · Cited by 7,083
The Principles of Humane Experimental Technique
(1960) · The Medical Journal of Australia · doi:10.5694/j.1326-5377.1960.tb73127.x · Cited by 5,045
Adverse outcome pathways: a conceptual framework to support ecotoxicology research and risk assessment
Ankley, G. T., Bennett, R. S., Erickson, R. J., Hoff, D. J., Hornung, M. W., Johnson, R. D., Mount, D. R., Nichols, J. W., Russom, C. L., Schmieder, P. K., et al. (2009) · Environmental Toxicology and Chemistry · doi:10.1002/etc.34 · Cited by 2,734
Human Respiratory Tract Model for Radiological Protection
Icrp (1994) · Medical Entomology and Zoology · Cited by 1,770
Carbon nanotubes show no sign of acute toxicity but induce intracellular reactive oxygen species in dependence on contaminants
Pulskamp, K., Diabaté, S., & Krug, H. F. (2006) · Toxicology Letters · doi:10.1016/j.toxlet.2006.11.001 · Cited by 1,014
Human Health Risk Assessment for Aluminium, Aluminium Oxide, and Aluminium Hydroxide
Krewski, D., Yokel, R. A., Nieboer, E., Borchelt, D., Cohen, J., Harry, J., Kacew, S., Lindsay, J., Mahfouz, A. M., & Rondeau, V. (2007) · Journal of Toxicology and Environmental Health, Part B · doi:10.1080/10937400701597766 · Cited by 998
Safety assessment for nanotechnology and nanomedicine: concepts of nanotoxicology
Oberdörster, G. (2009) · Journal of Internal Medicine · doi:10.1111/j.1365-2796.2009.02187.x · Cited by 943
Correlation between particle size, in vivo particle persistence, and lung injury.
Oberdörster, G., Ferin, J., & Lehnert, B. E. (1994) · Environmental Health Perspectives · doi:10.1289/ehp.102-1567252 · Cited by 936
Rare earth nanoparticles prevent retinal degeneration induced by intracellular peroxides
Chen, J., Patil, S. D., Seal, S., & McGinnis, J. F. (2006) · Nature Nanotechnology · doi:10.1038/nnano.2006.91 · Cited by 880

Show all 120 references

Research works printed but not linked: 6

Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18 December 2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH), establishing a European Chemicals Agency, amending Directive 1999/45/EC and repealing Council Regulation (EEC) No 793/93 and Commission Regulation (EC) No 1488/94 as well as Council Directive 76/769/EEC and Commission Directives 91/155/EEC, 93/67/EEC, 93/105/EC and 2000/21/EC. OJ L. 2006;396:1.
EU Commission recommendation on the definition of nanomaterial. OJ L 2011;275:38.
Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. OJ L. 2010;276:33.
Rehn B, Rehn S, Bruch J. Ein neues in vitro-Prüfkonzept (Vektorenmodell) zum biologischen Screening und Monitoring der Lungentoxizität von Stäuben. Gefahrstoffe—Reinhaltung der Luft. 1999;59:181–188.
Kuhlbusch TAJ, Krug HF, Nau K, editors. NanoCare—Health related effects of nanoparticles. Frankfurt: Final scientific report Dechema eV; 2009.
Schippritt D, Lipinski HG, Wiemann M. Measurement of nanoparticle uptake by alveolar macrophages: A new approach based on quantitative image analysis. In: Wohlleben W, Kuhlbusch T, Schnekenburger J, Lehr C-M, editors. Nanomaterials Throughout their Lifecycles: Safety. Boca Raton: Human Hazard and Exposure. Taylor & Francis; 2014.

Sorted by citations

Toxicity of graphene-family nanoparticles: a general review of the origins and mechanisms
Ou, L., Song, B., Liang, H., Liu, J., Feng, X., Deng, B., Sun, T., & Shao, L. (2016) · Particle and Fibre Toxicology · doi:10.1186/s12989-016-0168-y · Cited by 835
Safety Assessment of Graphene-Based Materials: Focus on Human Health and the Environment
Fadeel, B., Bussy, C., Merino, S., Vázquez, E., Emmanuel Flahaut, B., Mouchet, F., Evariste, L., Gauthier, L., Koivisto, A. J., Vogel, U. B., et al. (2018) · ACS Nano · doi:10.1021/acsnano.8b04758 · Cited by 693
In vitro approaches to assess the hazard of nanomaterials
Drašler, B., Sayre, P. G., Steinhäuser, K. G., Petri‐Fink, A., & Rothen‐Rutishauser, B. (2017) · NanoImpact · doi:10.1016/j.impact.2017.08.002 · Cited by 257
Recursive feature elimination in random forest classification supports nanomaterial grouping
Bahl, A., Hellack, B., Balaș, M., Dinischiotu, A., Wiemann, M., Brinkmann, J. F. F., Luch, A., Renard, B. Y., & Haase, A. (2019) · NanoImpact · doi:10.1016/j.impact.2019.100179 · Cited by 140
Self-citation
In vivo effects: Methodologies and biokinetics of inhaled nanomaterials
Oberdörster, G., & Kuhlbusch, T. A. J. (2017) · NanoImpact · doi:10.1016/j.impact.2017.10.007 · Cited by 107
Pathway-based predictive approaches for non-animal assessment of acute inhalation toxicity
Clippinger, A. J., Allen, D., Behrsing, H. P., BéruBé, K., Bolger, M. B., Casey, W., DeLorme, M. P., Gaça, M., Gehen, S. C., Glover, K., et al. (2018) · Toxicology in Vitro · doi:10.1016/j.tiv.2018.06.009 · Cited by 99
Self-citation
Alternative approaches for acute inhalation toxicity testing to address global regulatory and non-regulatory data requirements: An international workshop report
Clippinger, A. J., Allen, D., Jarabek, A. M., Corvaro, M., Gaça, M., Gehen, S. C., Hotchkiss, J. A., Patlewicz, G. Y., Melbourne, J., Hinderliter, P. M., et al. (2017) · Toxicology in Vitro · doi:10.1016/j.tiv.2017.12.011 · Cited by 104
Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote Organs
Wiemann, M., Vennemann, A., Blaske, F., Sperling, M., & Kärst, U. (2017) · Nanomaterials · doi:10.3390/nano7120441 · Cited by 86
Self-citation
Decision tree models to classify nanomaterials according to the DF4nanoGrouping scheme
Gajewicz, A., Puzyn, T., Odziomek, K., Urbaszek, P., Haase, A., Riebeling, C., Luch, A., Irfan, M. A., Landsiedel, R., van der Zande, M., et al. (2017) · Nanotoxicology · doi:10.1080/17435390.2017.1415388 · Cited by 85
Self-citation
Cytotoxicity screening and cytokine profiling of nineteen nanomaterials enables hazard ranking and grouping based on inflammogenic potential
Bhattacharya, K., Kiliç, G., Costa, P. M., & Fadeel, B. (2017) · Nanotoxicology · doi:10.1080/17435390.2017.1363309 · Cited by 89
Metal‐based nanomaterials and nanocomposites as promising frontier in cancer chemotherapy
Kumar, S., Shukla, M. K., Sharma, A. K., Jayaprakash, G. K., Tonk, R. K., Chellappan, D. K., Singh, S. K., Dua, K., Ahmed, F., Bhattacharyya, S., et al. (2023) · MedComm · doi:10.1002/mco2.253 · Cited by 77
An Integrated Approach to Testing and Assessment to Support Grouping and Read-Across of Nanomaterials After Inhalation Exposure
Braakhuis, H., Murphy, F. A., Ma‐Hock, L., Dekkers, S., Keller, J. G., Oomen, A. G., & Stone, V. (2021) · Applied In Vitro Toxicology · doi:10.1089/aivt.2021.0009 · Cited by 74
Self-citation
The protein corona suppresses the cytotoxic and pro-inflammatory response in lung epithelial cells and macrophages upon exposure to nanosilica
Leibe, R., Hsiao, I., Fritsch‐Decker, S., Kielmeier, U., Wågbø, A. M., Voss, B., Schmidt, A., Hessman, S. D., Duschl, A., Oostingh, G. J., et al. (2019) · Archives of Toxicology · doi:10.1007/s00204-019-02422-9 · Cited by 69
Effects of Sample Preparation on Particle Size Distributions of Different Types of Silica in Suspensions
Marín, R. R. R., Babick, F., Lindner, G., Wiemann, M., & Stintz, M. (2018) · Nanomaterials · doi:10.3390/nano8070454 · Cited by 68
Self-citation
Biocompatibility of Amine‐Functionalized Silica Nanoparticles: The Role of Surface Coverage
Hsiao, I., Fritsch‐Decker, S., Leidner, A., Al‐Rawi, M., Hug, V., Diabaté, S., Grage, S. L., Meffert, M., Stoeger, T., Gerthsen, D., et al. (2019) · Small · doi:10.1002/smll.201805400 · Cited by 68
Autophagy induced by silica nanoparticles protects RAW264.7 macrophages from cell death
Marquardt, C., Fritsch‐Decker, S., Al‐Rawi, M., Diabaté, S., & Weiß, C. (2017) · Toxicology · doi:10.1016/j.tox.2017.01.019 · Cited by 65
Pro-Inflammatory versus Immunomodulatory Effects of Silver Nanoparticles in the Lung: The Critical Role of Dose, Size and Surface Modification
Alessandrini, F., Vennemann, A., Gschwendtner, S., Neumann, A. U., Rothballer, M., Seher, T., Wimmer, M., Kublik, S., Traidl‐Hoffmann, C., Schloter, M., et al. (2017) · Nanomaterials · doi:10.3390/nano7100300 · Cited by 63
Self-citation
Nanoparticle induced barrier function assessment at liquid–liquid and air–liquid interface in novel human lung epithelia cell lines
Leibrock, L., Wagener, S., Singh, A. V., Laux, P., & Luch, A. (2019) · Toxicology Research · doi:10.1039/c9tx00179d · Cited by 63
Abiotic dissolution rates of 24 (nano)forms of 6 substances compared to macrophage-assisted dissolution and in vivo pulmonary clearance: Grouping by biodissolution and transformation
Koltermann-Jülly, J., Keller, J. G., Vennemann, A., Werle, K., Müller, P., Ma‐Hock, L., Landsiedel, R., Wiemann, M., & Wohlleben, W. (2018) · NanoImpact · doi:10.1016/j.impact.2018.08.005 · Cited by 61
Self-citation
Differences in the anatomy and physiology of the human and rat respiratory tracts and impact on toxicological assessments
Stucki, A., Sauer, U. G., Allen, D., Kleinstreuer, N., Perron, M. M., Yozzo, K., Lowit, A., & Clippinger, A. J. (2024) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2024.105648 · Cited by 56
Self-citation
Adoption of in vitro systems and zebrafish embryos as alternative models for reducing rodent use in assessments of immunological and oxidative stress responses to nanomaterials
Johnston, H. J., Verdon, R., Gillies, S., Brown, D. M., Fernandes, T. F., Henry, T. B., Rossi, A. G., Tran, L., Tucker, C. S., Tyler, C. R., et al. (2017) · Critical Reviews in Toxicology · doi:10.1080/10408444.2017.1404965 · Cited by 54
Predicting the in vivo pulmonary toxicity induced by acute exposure to poorly soluble nanomaterials by using advanced in vitro methods
Loret, T., Rogerieux, F., Trouiller, B., Braun, A., Egles, C., & Lacroix, G. (2018) · Particle and Fibre Toxicology · doi:10.1186/s12989-018-0260-6 · Cited by 53
Aligning nanotoxicology with the 3Rs: What is needed to realise the short, medium and long-term opportunities?
Burden, N., Aschberger, K., Chaudhry, Q., Clift, M. J. D., Fowler, P., Johnston, H. J., Landsiedel, R., Rowland, J., Stone, V., & Doak, S. H. (2017) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2017.10.021 · Cited by 52
Self-citation
Safety assessment of nanomaterials using an advanced decision-making framework, the DF4nanoGrouping
Landsiedel, R., Ma‐Hock, L., Wiench, K., Wohlleben, W., & Sauer, U. G. (2017) · Journal of Nanoparticle Research · doi:10.1007/s11051-017-3850-6 · Cited by 51
Self-citation
Dry Generation of CeO2 Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface—Dosimetry Considerations and Comparison to Submerged Exposure
Cappellini, F., Bucchianico, S. D., Karri, V., Latvala, S., Malmlöf, M., Kippler, M. J., Elihn, K., Hedberg, J. F., Wallinder, I. O., Gerde, P., et al. (2020) · Nanomaterials · doi:10.3390/nano10040618 · Cited by 50
Air–Liquid Interface Exposure of Lung Epithelial Cells to Low Doses of Nanoparticles to Assess Pulmonary Adverse Effects
Diabaté, S., Armand, L., Murugadoss, S., Dilger, M., Fritsch‐Decker, S., Schlager, C., Beal, D. M., Arnal, M.-É., Biola-Clier, M., Ambrose, S. V. Y., et al. (2020) · Nanomaterials · doi:10.3390/nano11010065 · Cited by 50
The nanoGRAVUR framework to group (nano)materials for their occupational, consumer, environmental risks based on a harmonized set of material properties, applied to 34 case studies
Wohlleben, W., Hellack, B., Nickel, C., Herrchen, M., Hund‐Rinke, K., Kettler, K., Riebeling, C., Haase, A., Funk, B., Kühnel, D., et al. (2019) · Nanoscale · doi:10.1039/c9nr03306h · Cited by 48
Self-citation
A multi-omics approach reveals mechanisms of nanomaterial toxicity and structure–activity relationships in alveolar macrophages
Bannuscher, A., Karkossa, I., Buhs, S., Nollau, P., Kettler, K., Balaș, M., Dinischiotu, A., Hellack, B., Wiemann, M., Luch, A., et al. (2019) · Nanotoxicology · doi:10.1080/17435390.2019.1684592 · Cited by 44
Self-citation
Toxicity assessment of metal oxide nanomaterials using in vitro screening and murine acute inhalation studies
Areecheewakul, S., Adamcakova‐Dodd, A., Givens, B. E., Steines, B. R., Wang, Y., Meyerholz, D. K., Parizek, N. J., Altmaier, R. W., Haque, E., O’Shaughnessy, P. T., et al. (2020) · NanoImpact · doi:10.1016/j.impact.2020.100214 · Cited by 43
Perilous paradigm of graphene oxide and its derivatives in biomedical applications: Insight to immunocompatibility
Ayreen, Z., Khatoon, U., Kirti, A., Sinha, A., Gupta, A., Lenka, S. S., Yadav, A., Mohanty, R., Naser, S. S., Mishra, R., et al. (2024) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2024.116842 · Cited by 42
Nanomaterial categorization by surface reactivity: A case study comparing 35 materials with four different test methods
Bahl, A., Hellack, B., Wiemann, M., Giusti, A., Werle, K., Haase, A., & Wohlleben, W. (2020) · NanoImpact · doi:10.1016/j.impact.2020.100234 · Cited by 44
Self-citation
Review of New Approach Methodologies for Application in Risk Assessment of Nanoparticles in the Food and Feed Sector: Status and Challenges
Usmani, S. M., Bremer‐Hoffmann, S., Cheyns, K., Cubadda, F., Dumit, V. I., Escher, S. E., Fessard, V., Gutleb, A. C., Léger, T., Liu, Y., et al. (2024) · EFSA Supporting Publications · doi:10.2903/sp.efsa.2024.en-8826 · Cited by 47
Subtoxic cell responses to silica particles with different size and shape
Kersting, M., Olejnik, M., Rosenkranz, N., Loza, K., Breisch, M., Rostek, A., Westphal, G., Bünger, J., Ziegler, N., Ludwig, A., et al. (2020) · Scientific Reports · doi:10.1038/s41598-020-78550-5 · Cited by 43
Human lung cell models to study aerosol delivery – considerations for model design and development
Rothen‐Rutishauser, B., Gibb, M., He, R., Petri‐Fink, A., & Sayes, C. M. (2022) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2022.106337 · Cited by 39
An inter-laboratory effort to harmonize the cell-delivered in vitro dose of aerosolized materials
Bannuscher, A., Schmid, O., Drašler, B., Rohrbasser, A., Braakhuis, H., Meldrum, K., Zwart, E., Gremmer, E. R., Birk, B., Rissel, M., et al. (2022) · NanoImpact · doi:10.1016/j.impact.2022.100439 · Cited by 38
Self-citation
Methodological considerations when conducting in vitro , air–liquid interface exposures to engineered nanoparticle aerosols
Secondo, L. E., Liu, N. J., & Lewinski, N. A. (2016) · Critical Reviews in Toxicology · doi:10.1080/10408444.2016.1223015 · Cited by 38
Pulmonary toxicity in rats following inhalation exposure to poorly soluble particles: The issue of impaired clearance and the relevance for human health hazard and risk assessment
Bos, P. M. J., Gosens, I., Geraets, L., Delmaar, C. J. E., & Cassee, F. R. (2019) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2019.104498 · Cited by 38
Surface reactivity measurements as required for grouping and read-across: An advanced FRAS protocol
Gandon, A., Werle, K., Neubauer, N., & Wohlleben, W. (2017) · Journal of Physics Conference Series · doi:10.1088/1742-6596/838/1/012033 · Cited by 36
Refining in vitro models for nanomaterial exposure to cells and tissues
Guggenheim, E. J., Milani, S., Röttgermann, P. J. F., Dusinska, M., Saout, C., Salvati, A., Rädler, J. O., & Lynch, I. (2018) · NanoImpact · doi:10.1016/j.impact.2018.02.008 · Cited by 36
Toxicity mechanism of engineered nanomaterials: Focus on mitochondria
Yao, Y., Zhang, T., & Tang, M. X. (2023) · Environmental Pollution · doi:10.1016/j.envpol.2023.123231 · Cited by 35
Parametric Optimization of an Air–Liquid Interface System for Flow-Through Inhalation Exposure to Nanoparticles: Assessing Dosimetry and Intracellular Uptake of CeO2 Nanoparticles
Leibrock, L., Jungnickel, H., Tentschert, J., Katz, A., Toman, B., Petersen, E. J., Bierkandt, F. S., Singh, A. V., Laux, P., & Luch, A. (2020) · Nanomaterials · doi:10.3390/nano10122369 · Cited by 34
Comparison of quantitation methods in proteomics to define relevant toxicological information on AhR activation of HepG2 cells by BaP
Wang, Z., Karkossa, I., Großkopf, H., Rolle-Kampczyk, U. E., Jörg Hackermüller, A., von Bergen�, M., & Schubert, K. A. (2020) · Toxicology · doi:10.1016/j.tox.2020.152652 · Cited by 32
The Use of Nanomaterial In Vivo Organ Burden Data for In Vitro Dose Setting
Ma‐Hock, L., Sauer, U. G., Ruggiero, E., Keller, J. G., Wohlleben, W., & Landsiedel, R. (2021) · Small · doi:10.1002/smll.202005725 · Cited by 33
Self-citation
Assessing the in vitro toxicity of airborne (nano)particles to the human respiratory system: from basic to advanced models
Bessa, M. J., Brandão, F., Rosário, F., Moreira, L., Reis, A. T., Valdiglesias, V., Laffón, B., Fraga, S., & Teixeira, J. P. (2023) · Journal of Toxicology and Environmental Health Part B · doi:10.1080/10937404.2023.2166638 · Cited by 36
Revisiting the stress paradigm for silica nanoparticles: decoupling of the anti-oxidative defense, pro-inflammatory response and cytotoxicity
Fritsch‐Decker, S., Marquardt, C., Stoeger, T., Diabaté, S., & Weiß, C. (2018) · Archives of Toxicology · doi:10.1007/s00204-018-2223-y · Cited by 32
An in-depth multi-omics analysis in RLE-6TN rat alveolar epithelial cells allows for nanomaterial categorization
Karkossa, I., Bannuscher, A., Hellack, B., Bahl, A., Buhs, S., Nollau, P., Luch, A., Schubert, K. A., von Bergen�, M., & Haase, A. (2019) · Particle and Fibre Toxicology · doi:10.1186/s12989-019-0321-5 · Cited by 32
A quantitative framework to group nanoscale and microscale particles by hazard potency to derive occupational exposure limits: Proof of concept evaluation
Drew, N. M., Kuempel, E. D., Pei, Y., & Yang, F. (2017) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2017.08.003 · Cited by 30
In Vitro and In Vivo Short-Term Pulmonary Toxicity of Differently Sized Colloidal Amorphous SiO2
Wiemann, M., Sauer, U. G., Vennemann, A., Bäcker, S., Keller, J. G., Ma‐Hock, L., Wohlleben, W., & Landsiedel, R. (2018) · Nanomaterials · doi:10.3390/nano8030160 · Cited by 30
Self-citation
Effects of Ultrasonic Dispersion Energy on the Preparation of Amorphous SiO2 Nanomaterials for In Vitro Toxicity Testing
Wiemann, M., Vennemann, A., Stintz, M., Marín, R. R. R., Babick, F., Lindner, G., Schuster, T., Brinkmann, U., & Krueger, N. (2018) · Nanomaterials · doi:10.3390/nano9010011 · Cited by 30
Self-citation
Nanomaterials induce different levels of oxidative stress, depending on the used model system: Comparison of in vitro and in vivo effects
Karkossa, I., Bannuscher, A., Hellack, B., Wohlleben, W., Laloy, J., Stan, M. S., Dinischiotu, A., Wiemann, M., Luch, A., Haase, A., et al. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.149538 · Cited by 30
Self-citation
Barium sulfate micro- and nanoparticles as bioinert reference material in particle toxicology
Loza, K., Föhring, I., Bünger, J., Westphal, G., Köller, M., Epple, M., & Sengstock, C. (2016) · Nanotoxicology · doi:10.1080/17435390.2016.1235740 · Cited by 29
Comparing α-Quartz-Induced Cytotoxicity and Interleukin-8 Release in Pulmonary Mono- and Co-Cultures Exposed under Submerged and Air-Liquid Interface Conditions
Friesen, A., Fritsch‐Decker, S., Hufnagel, M., Mülhopt, S., Stapf, D., Hartwig, A., & Weiß, C. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23126412 · Cited by 28
Differences in the toxicity of cerium dioxide nanomaterials after inhalation can be explained by lung deposition, animal species and nanoforms
Dekkers, S., Ma‐Hock, L., Lynch, I., Russ, M., Miller, M. R., Schins, R. P. F., Keller, J., Römer, I., Küttler, K., Strauss, V., et al. (2018) · Inhalation Toxicology · doi:10.1080/08958378.2018.1516834 · Cited by 28
Self-citation
Rationale and decision rules behind the ECETOC NanoApp to support registration of sets of similar nanoforms within REACH
Janer, G., Landsiedel, R., & Wohlleben, W. (2020) · Nanotoxicology · doi:10.1080/17435390.2020.1842933 · Cited by 28
Self-citation
Systematic Review of Multi-Omics Approaches to Investigate Toxicological Effects in Macrophages
Karkossa, I., Raps, S., von Bergen�, M., & Schubert, K. A. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21249371 · Cited by 29
Protein and lipid homeostasis altered in rat macrophages after exposure to metallic oxide nanoparticles
Doumandji, Z. M., Safar, R., Leroux, M., Nahle, S., Cassidy, H., Matallanas, D., Rihn, B. H., Ferrari, L., & Joubert, O. (2019) · Cell Biology and Toxicology · doi:10.1007/s10565-019-09484-6 · Cited by 28
Combined In Vitro and In Vivo Approaches to Propose a Putative Adverse Outcome Pathway for Acute Lung Inflammation Induced by Nanoparticles: A Study on Carbon Dots
Weiss, M., Fan, J., Claudel, M., Lebeau, L., Pons, F., & Ronzani, C. (2021) · Nanomaterials · doi:10.3390/nano11010180 · Cited by 26
Differential Effects of Surface-Functionalized Zirconium Oxide Nanoparticles on Alveolar Macrophages, Rat Lung, and a Mouse Allergy Model
Vennemann, A., Alessandrini, F., & Wiemann, M. (2017) · Nanomaterials · doi:10.3390/nano7090280 · Cited by 26
Self-citation
A cross-species and model comparison of the acute toxicity of nanoparticles used in the pigment and ink industries
Brown, D. M., Johnston, H. J., Gaiser, B. K., Pinna, N., Caputo, G., Çulha, M., Keleştemur, S., Altunbek, M., Stone, V., Roy, J. C., et al. (2018) · NanoImpact · doi:10.1016/j.impact.2018.02.001 · Cited by 27
Genes expression profiling of alveolar macrophages exposed to non-functionalized, anionic and cationic multi-walled carbon nanotubes shows three different mechanisms of toxicity
Nahle, S., Cassidy, H., Leroux, M. M., Mercier, R., Ghanbaja, J., Doumandji, Z., Matallanas, D., Rihn, B., Joubert, O., & Ferrari, L. (2020) · Journal of Nanobiotechnology · doi:10.1186/s12951-020-0587-7 · Cited by 24
Kinetics of chemotaxis, cytokine, and chemokine release of NR8383 macrophages after exposure to inflammatory and inert granular insoluble particles
Schremmer, I., Brik, A., Weber, D. G., Rosenkranz, N., Rostek, A., Loza, K., Brüning, T., Johnen, G., Epple, M., Bünger, J., et al. (2016) · Toxicology Letters · doi:10.1016/j.toxlet.2016.08.014 · Cited by 25
Time-resolved characterization of the mechanisms of toxicity induced by silica and amino-modified polystyrene on alveolar-like macrophages
Deville, S., Honrath, B., Tran, Q. T. D., Fejér, G., Lambrichts, I., Nelissen, I., Dolga, A. M., & Salvati, A. L. (2019) · Archives of Toxicology · doi:10.1007/s00204-019-02604-5 · Cited by 25
Graphite particles induce ROS formation in cell free systems and human cells
Zerbi, G., Barbon, A., Bengalli, R. D., Lucotti, A., Catelani, T., Tampieri, F., Gualtieri, M., D’Arienzo, M., Morazzoni, F., & Camatini, M. (2017) · Nanoscale · doi:10.1039/c7nr02540h · Cited by 24
Specific Surface Modifications of Silica Nanoparticles Diminish Inflammasome Activation and In Vivo Expression of Selected Inflammatory Genes
Marzaioli, V., Groß, C. J., Weichenmeier, I., Schmidt‐Weber, C. B., Gutermuth, J., Groß, O., & Alessandrini, F. (2017) · Nanomaterials · doi:10.3390/nano7110355 · Cited by 23
Silica Nanoparticles Provoke Cell Death Independent of p53 and BAX in Human Colon Cancer Cells
Fritsch‐Decker, S., An, Z., Yan, J., Hansjosten, I., Al‐Rawi, M., Peravali, R., Diabaté, S., & Weiß, C. (2019) · Nanomaterials · doi:10.3390/nano9081172 · Cited by 23
Use of Cause-and-Effect Analysis to Optimize the Reliability of In Vitro Inhalation Toxicity Measurements Using an Air–Liquid Interface
Petersen, E. J., Sharma, M., Clippinger, A. J., Gordon, J., Katz, A., Laux, P., Leibrock, L., Luch, A., Matheson, J. M., Stucki, A., et al. (2021) · Chemical Research in Toxicology · doi:10.1021/acs.chemrestox.1c00080 · Cited by 22
Interaction of rat alveolar macrophages with dental composite dust
LANDUYT, K. L. V., Čokić, S. M., Asbach, C., Hoet, P., Godderis, L., Reichl, F., MEERBEEK, B. V., Vennemann, A., & Wiemann, M. (2016) · Particle and Fibre Toxicology · doi:10.1186/s12989-016-0174-0 · Cited by 22
Self-citation
Lung Toxicity Analysis of Nano-Sized Kaolin and Bentonite: Missing Indications for a Common Grouping
Wiemann, M., Vennemann, A., & Wohlleben, W. (2020) · Nanomaterials · doi:10.3390/nano10020204 · Cited by 22
Self-citation
Revealing Silver Nanoparticle Uptake by Macrophages Using SR-μXRF and LA-ICP-MS
Reifschneider, O., Vennemann, A., Buzanich, G., Radtke, M., Reinholz, U., Riesemeier, H., Hogeback, J., Köppen, C., Großgarten, M., Sperling, M., et al. (2020) · Chemical Research in Toxicology · doi:10.1021/acs.chemrestox.9b00507 · Cited by 21
Self-citation
Multi-walled carbon nanotubes induce stronger migration of inflammatory cells in vitro than asbestos or granular particles but a similar pattern of inflammatory mediators
Westphal, G., Rosenkranz, N., Brik, A., Weber, D. G., Föhring, I., Monsé, C., Kaiser, N., Hellack, B., Mattenklott, M., Brüning, T., et al. (2019) · Toxicology in Vitro · doi:10.1016/j.tiv.2019.03.036 · Cited by 20
Toxicity of TiO2 Nanoparticles: Validation of Alternative Models
Leroux, M., Doumandji, Z. M., Chézeau, L., Gaté, L., Nahle, S., Hocquel, R., Zhernovkov, V., Migot, S., Ghanbaja, J., Bonnet, C., et al. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21144855 · Cited by 20
Evaluation of the NLRP3 Inflammasome Activating Effects of a Large Panel of TiO2 Nanomaterials in Macrophages
Kolling, J., Tigges, J., Hellack, B., Albrecht, C., & Schins, R. P. F. (2020) · Nanomaterials · doi:10.3390/nano10091876 · Cited by 20
Aerogels are not regulated as nanomaterials, but can be assessed by tiered testing and grouping strategies for nanomaterials
Keller, J. G., Wiemann, M., Gröters, S., Werle, K., Vennemann, A., Landsiedel, R., & Wohlleben, W. (2021) · Nanoscale Advances · doi:10.1039/d1na00044f · Cited by 19
Self-citation
A Low-Serum Culture System for Prolonged in Vitro Toxicology Experiments on a Macrophage System
Dalzon, B., Torres, A., Devcic, J., Fenel, D., Sergent, J., & Rabilloud, T. (2021) · Frontiers in Toxicology · doi:10.3389/ftox.2021.780778 · Cited by 20
Immediate and Sustained Effects of Cobalt and Zinc-Containing Pigments on Macrophages
Devcic, J., Dussol, M., Collin‐Faure, V., Pérard, J., Fenel, D., Schoehn, G., Carrière, M., Rabilloud, T., & Dalzon, B. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.865239 · Cited by 19
Predicting nanomaterials pulmonary toxicity in animals by cell culture models: Achievements and perspectives
Ianni, E. D., Jacobsen, N. R., Vogel, U. B., & Möller, P. (2022) · Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology · doi:10.1002/wnan.1794 · Cited by 19
Phosphonate coating of SiO2 nanoparticles abrogates inflammatory effects and local changes of the lipid composition in the rat lung: a complementary bioimaging study
Großgarten, M., Holzlechner, M., Vennemann, A., Balbekova, A., Wieland, K., Sperling, M., Lendl, B., Marchetti‐Deschmann, M., Kärst, U., & Wiemann, M. (2018) · Particle and Fibre Toxicology · doi:10.1186/s12989-018-0267-z · Cited by 18
Self-citation
The influence of exposure approaches to in vitro lung epithelial barrier models to assess engineered nanomaterial hazard
Meldrum, K., Evans, S. J., Vogel, U. B., Tran, L., Doak, S. H., & Clift, M. J. D. (2022) · Nanotoxicology · doi:10.1080/17435390.2022.2051627 · Cited by 17
Morphometric Characterization of Rat and Human Alveolar Macrophage Cell Models and their Response to Amiodarone using High Content Image Analysis
Hoffman, E., Patel, A., Ball, D. I., Klapwijk, J. C., Millar, V., Kumar, A., Martin, A., Mahendran, R., Dailey, L. A., Forbes, B., et al. (2017) · Pharmaceutical Research · doi:10.1007/s11095-017-2176-5 · Cited by 17
Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation and Inhalation of Different Variants of Amorphous Silica Nanomaterials (aSiO2 NM)
Brandão, F., Costa, C., Bessa, M. J., Dumortier, E., Debacq‐Chainiaux, F., Hubaux, R., Salmon, M., Laloy, J., Stan, M. S., Hermenean, A. O., et al. (2021) · Nanomaterials · doi:10.3390/nano11061502 · Cited by 16
Carbon nanotubes: A review on risks assessment, mechanism of toxicity and future directives to prevent health implication
Babele, P. K., Verma, M. K., & Bhatia, R. K. (2021) · Biocell · doi:10.32604/biocell.2021.013409 · Cited by 16
Resolution of MoS 2 Nanosheets‐Induced Pulmonary Inflammation Driven by Nanoscale Intracellular Transformation and Extracellular‐Vesicle Shuttles
Peña, N. O., Cherukula, K., Even, B., Ji, D., Razafindrakoto, S., Peng, S., Silva, A., Ménard‐Moyon, C., Hillaireau, H., Bianco, A., et al. (2023) · Advanced Materials · doi:10.1002/adma.202209615 · Cited by 15
Pro-inflammatory and genotoxic responses by metal oxide nanomaterials in alveolar epithelial cells and macrophages in submerged condition and air-liquid interface: An in vitro-in vivo correlation study
Ianni, E. D., Erdem, J. S., Narui, S. Z., Wallin, H., Lynch, I., Vogel, U. B., Jacobsen, N. R., & Möller, P. (2024) · Toxicology in Vitro · doi:10.1016/j.tiv.2024.105897 · Cited by 15
Classes of organic pigments meet tentative PSLT criteria and lack toxicity in short-term inhalation studies
Stratmann, H., Wohlleben, W., Wiemann, M., Vennemann, A., End, N., Veith, U., Ma‐Hock, L., & Landsiedel, R. (2021) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2021.104988 · Cited by 13
Self-citation
Use of new approach methodologies (NAMs) to meet regulatory requirements for the assessment of tobacco and other nicotine-containing products
Miller‐Holt, J., Behrsing, H. P., Clippinger, A. J., Hirn, C., Stedeford, T. J., & Stucki, A. (2022) · Frontiers in Toxicology · doi:10.3389/ftox.2022.943358 · Cited by 13
Cell-Biological Response and Sub-Toxic Inflammatory Effects of Titanium Dioxide Particles with Defined Polymorphic Phase, Size, and Shape
Breisch, M., Olejnik, M., Loza, K., Prymak, O., Rosenkranz, N., Bünger, J., Sengstock, C., Köller, M., Westphal, G., & Epple, M. (2023) · Nanomaterials · doi:10.3390/nano13101621 · Cited by 12
Possibilities to group nanomaterials across different substances – A case study on organic pigments
Jeliazkova, N., Ma‐Hock, L., Janer, G., Stratmann, H., & Wohlleben, W. (2022) · NanoImpact · doi:10.1016/j.impact.2022.100391 · Cited by 12
Self-citation
LA-ICP-MS and Immunohistochemical Staining with Lanthanide-Labeled Antibodies to Study the Uptake of CeO2 Nanoparticles by Macrophages in Tissue Sections
Seiffert, S. B., Vennemann, A., Nordhorn, I. D., Kröger, S., Wiemann, M., & Kärst, U. (2022) · Chemical Research in Toxicology · doi:10.1021/acs.chemrestox.1c00433 · Cited by 11
Self-citation
Updated Aspects of Safety Regulations for Biomedical Applications of Aerogel Compounds—Compendia-Like Evaluation
Neagu, M. T., Grisi, F., Pulvirenti, A., Simón‐Vázquez, R., García‐González, C. A., & Boccia, A. C. (2023) · Safety · doi:10.3390/safety9040080 · Cited by 12
Risk Assessment and Risk Management
Landsiedel, R., Sauer, U. G., & de Jong, W. H. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-809199-9.00008-2 · Cited by 11
Self-citation
Assessing biological oxidative damage induced by graphene-based materials: An asset for grouping approaches using the FRAS assay
Achawi, S., Feneon, B., Pourchez, J., & Forest, V. (2021) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2021.105067 · Cited by 10
Serum Lowers Bioactivity and Uptake of Synthetic Amorphous Silica by Alveolar Macrophages in a Particle Specific Manner
Wiemann, M., Vennemann, A., Venzago, C., Lindner, G., Schuster, T., & Krueger, N. (2021) · Nanomaterials · doi:10.3390/nano11030628 · Cited by 10
Self-citation
Application of Cell Membrane-Coated Nanomaterials for Tumor Treatment
Zhu, Y., Cui, H., Zhang, J. C., Bei, Y., Huang, Y., Li, M., Liu, J., Wu, Y., & Gao, J. (2023) · Mini-Reviews in Medicinal Chemistry · doi:10.2174/1389557523666230203145645 · Cited by 9
PLATOX: Integrated In Vitro/In Vivo Approach for Screening of Adverse Lung Effects of Graphene-Related 2D Nanomaterials
Creutzenberg, O., Oliveira, H., Farcal, L. R., Schaudien, D., Mendes, A., Menezes, A. C., Tischler, T., Burla, S., & Ziemann, C. (2022) · Nanomaterials · doi:10.3390/nano12081254 · Cited by 9
Towards characterization of cell culture conditions for reliable proteomic analysis: in vitro studies on A549, differentiated THP-1, and NR8383 cell lines
Ledwith, R., Stobernack, T., Bergert, A., Bahl, A., Pink, M., Haase, A., & Dumit, V. I. (2024) · Archives of Toxicology · doi:10.1007/s00204-024-03858-4 · Cited by 8
Key Role of Choline Head Groups in Large Unilamellar Phospholipid Vesicles for the Interaction with and Rupture by Silica Nanoparticles
Leibe, R., Fritsch‐Decker, S., Gußmann, F., Wågbø, A. M., Wadhwani, P., Diabaté, S., Wenzel, W., Ulrich, A. S., & Weiß, C. (2023) · Small · doi:10.1002/smll.202207593 · Cited by 8
In vitro assessment of the pulmonary toxicity of particulate matter emitted during haze events in Chiang Mai, Thailand via investigation of macrophage responses
Maciaszek, K., Gillies, S. L. J., Kawichai, S., Prapamontol, T., Santijitpakdee, T., Kliengchuay, W., Sahanavin, N., Mueller, W., Vardoulakis, S., Samutrtai, P., et al. (2022) · Environmental Research Health · doi:10.1088/2752-5309/ac9748 · Cited by 7
Application of in vitro pulmonary models for hazard screening of silica particles
Ruijter, N., Braakhuis, H., Katsumiti, A., Garriz, I. P., Carrière, M., Zanoni, I., Candalija, A., Marshall, J., Vermeulen, J. P., Cassee, F. R., et al. (2025) · Archives of Toxicology · doi:10.1007/s00204-025-04100-5 · Cited by 6
In chemico methodology for engineered nanomaterial categorization according to number, nature and oxidative potential of reactive surface sites
Alcolea-Rodriguez, V., Portela, R., Casilda, V. C., & Bañares, M. Á. (2024) · Environmental Science Nano · doi:10.1039/d3en00810j · Cited by 6
Evaluation of a bioprinted 3D airway tissue model for toxicity testing of nanomaterials; Pathway to integration into a tiered testing strategy for hazard assessment to support safety-by-design
Lee, Y., McAllister, K., Lee, H., Jung, S., & Murphy, F. A. (2025) · Nano Today · doi:10.1016/j.nantod.2025.102655 · Cited by 6

Authors: Wiemann, M., Vennemann, A., Sauer, U. G., Wiench, K., Ma-Hock, L., & Landsiedel, R.
Affiliations: BASF (Germany) (DE)
Corresponding author: Wiemann, Martin
ORCID: Wiemann, Martin
Vennemann, Antje
Sauer, Ursula G.
Wiench, Karin
Landsiedel, Robert
Venue: Journal of Nanobiotechnology
Publisher: Springer Science and Business Media LLC
Published: 2016-03-05
Volume: 14
Issue: 1
Pages: 16-16
ISSN: 1477-3155 (ISSN-L)
DOI: 10.1186/s12951-016-0164-2
PMC: PMC4779246
OpenAlex: W2290248210
Cited by: 152
References: 120
Citations by year: 2026: 6 · 2025: 13 · 2024: 12 · 2023: 12 · 2022: 18 · 2021: 14 · 2020: 18 · 2019: 18 · 2018: 17 · 2017: 18 · 2016: 6
FWCI: 8.76
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
Locations: Journal of Nanobiotechnology (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Funders: Bundesministerium für Bildung und Forschung (03X0105, 0315483A)
Topics: Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Occupational and environmental lung diseases
Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
Keywords: alveolar macrophages, NR8383 cells, in vitro-in vivo correlation, zirconia nanoparticles, amorphous silica nanoparticles, surface functionalization, hazard assessment, cytotoxicity, oxidative stress
MeSH terms: Animals, Cell Line, Ferric Compounds, Graphite, Hydrogen Peroxide, Lung, Particle Size, Silicon Dioxide, Titanium, Macrophages, Alveolar, Inhalation Exposure, Nanostructures, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

An in vitro alveolar macrophage assay for predicting the short-term inhalation toxicity of nanomaterials

Abstract

Overview

Integrity signals

Figures

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks