Cited by 198Nanoparticle-Based Drug DeliveryExtracellular vesicles in diseaseRheumatoid Arthritis Research and Therapies

Exosome-based biomimetic nanoparticles targeted to inflamed joints for enhanced treatment of rheumatoid arthritis

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Yan, Feili, Zhong, Zhirong, Wang, Yao, Feng, Yue, Mei, Zhiqiang, Li, Hui, Chen, Xiang, Cai, Liang +1 more·2020·Journal of Nanobiotechnology

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Abstract

BACKGROUND: Glucocorticoids (GCs) show powerful treatment effect on rheumatoid arthritis (RA). However, the clinical application is limited by their nonspecific distribution after systemic administration, serious adverse reactions during long-term administration. To achieve better treatment, reduce side effect, we here established a biomimetic exosome (Exo) encapsulating dexamethasone sodium phosphate (Dex) nanoparticle (Exo/Dex), whose surface was modified with folic acid (FA)-polyethylene glycol (PEG)-cholesterol (Chol) compound to attain FPC-Exo/Dex active targeting drug delivery system. RESULTS: The size of FPC-Exo/Dex was 128.43 ± 16.27 nm, with a polydispersity index (PDI) of 0.36 ± 0.05, and the Zeta potential was - 22.73 ± 0.91 mV. The encapsulation efficiency (EE) of the preparation was 10.26 ± 0.73%, with drug loading efficiency (DLE) of 18.81 ± 2.05%. In vitro study showed this system displayed enhanced endocytosis and excellent anti-inflammation effect against RAW264.7 cells by suppressing pro-inflammatory cytokines and increasing anti-inflammatory cytokine. Further biodistribution study showed the fluorescence intensity of FPC-Exo/Dex was stronger than other Dex formulations in joints, suggesting its enhanced accumulation to inflammation sites. In vivo biodistribution experiment displayed FPC-Exo/Dex could preserve the bone and cartilage of CIA mice better and significantly reduce inflamed joints. Next in vivo safety evaluation demonstrated this biomimetic drug delivery system had no obvious hepatotoxicity and exhibited desirable biocompatibility. CONCLUSION: The present study provides a promising strategy for using exosome as nanocarrier to enhance the therapeutic effect of GCs against RA.

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  • Engineered exosomes modified with folic acid, PEG, and cholesterol loaded with dexamethasone sodium phosphate better protected bone and cartilage in CIA mice and reduced joint inflammation without obvious liver toxicity.

    “In vivo biodistribution experiments showed that these engineered exosomes better protected bone and cartilage in CIA mice and reduced joint inflammation without obvious liver toxicity.”

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Authors
Yan, F., Zhong, Z., Wang, Y., Feng, Y., Mei, Z., Li, H., Chen, X., Cai, L., & Li, C.
Affiliations
  • Southwest Medical University (CN)
  • Affiliated Hospital of Southwest Medical University (CN)
  • Sichuan University (CN)
Corresponding author
  • Cai, Liang
  • Li, Chunhong
Venue
Journal of Nanobiotechnology
Published
2020-08-20
Volume
18
Issue
1
Pages
115-115
ISSN
1477-3155 (ISSN-L)
OpenAlex
W3050111167
Cited by
197
References
54
Citations by year
2026: 18 · 2025: 36 · 2024: 43 · 2023: 36 · 2022: 39 · 2021: 24 · 2020: 1
FWCI
6.50
Type
journal article
Language
en
Open access
Locations
  • Journal of Nanobiotechnology (publishedVersion, cc-by)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Major Science and Technology Projects of China (81803478)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Health Sciences › Medicine › Rheumatology › Rheumatoid Arthritis Research and Therapies
Keywords
rheumatoid arthritis, exosomes, biomimetic nanoparticles, dexamethasone, glucocorticoids, targeted drug delivery, folic acid, joint inflammation, anti-inflammatory cytokines, collagen-induced arthritis, tissue distribution, biocompatibility, nanocarriers
MeSH terms
RAW 264.7 Cells, Animals, Anti-Inflammatory Agents, Arthritis, Rheumatoid, Cholesterol, Dexamethasone, Folic Acid, Joints, Male, Polyethylene Glycols, Biomimetic Materials, Mice, Nanoparticles, Exosomes

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