Synergistic activity of gold nanoparticles with amphotericin B on persister cells of Candida tropicalis biofilms
Open accessAbstract
Aim: The antifungal activity was studied on sessile and persister cells (PCs) of Candida tropicalis biofilms of gold nanoparticles (AuNPs) stabilized with cetyltrimethylammonium bromide (CTAB-AuNPs) and those conjugated with cysteine, in combination with Amphotericin B (AmB). Materials/methods: The PC model was used and synergistic activity was tested by the checkerboard assay. Biofilms were studied by crystal violet and scanning electron microscopy. Results/Conclusions: After the combination of both AuNPs and AmB the biofilm biomass was reduced, with significant differences in architecture being observed with a reduced biofilm matrix. In addition, the CTAB-AuNPs-AmB combination significantly reduced PCs. Understanding how these AuNPs aid in the fight against biofilms and the development of new approaches to eradicate PCs has relevance for chronic infection treatment.
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Inhibiting biofilms helps eradicate C. tropicalis persister cells.
“Inhibiting biofilms helps eradicate C. tropicalis persister cells [126].”
From Insight into the Mechanisms and Clinical Relevance of Antifungal Heteroresistance (2025) · cites this paper as [126]
The Discussion (page unavailable) says that gold nanoparticles combined with amphotericin B damaged C. tropicalis biofilm architecture, thereby decreasing the number of persister cells. This supports a link between disrupting biofilms and reducing persister cells, but the paper does not show that biofilm inhibition generally eradicates them; it reports a decrease with a specific combination treatment. (LLM, conf=0.88)
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Self-citation
- Authors
- Dasilva, M. A., Andrada, K. F. C., Torales, M. M., Hughes, I. M., Pez, P., García-Martínez, J. C., & Paraje, M. G.
- Affiliations
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Universidad Nacional de Córdoba (AR)
- University of Castilla-La Mancha (ES)
- Corresponding author
- Paraje, María Gabriela
- Venue
- Journal of Nanobiotechnology
- Published
- 2024-05-16
- Volume
- 22
- Issue
- 1
- Pages
- 254-254
- ISSN
- 1477-3155 (ISSN-L)
- PMC
- PMC11097580
- OpenAlex
- W4396975652
- Cited by
- 16
- References
- 53
- Citations by year
- 2026: 6 · 2025: 9 · 2024: 1
- FWCI
- 5.50
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Journal of Nanobiotechnology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Greater South Information System
- Funders
- Consejo Nacional de Investigaciones Científicas y Técnicas (PIP)
- Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (2021)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Keywords
- gold nanoparticles, amphotericin B, Candida tropicalis, biofilm, persister cells, antifungal synergy, biofilm matrix, chronic fungal infection
- MeSH terms
- Cetrimonium, Amphotericin B, Antifungal Agents, Cetrimonium Compounds, Drug Synergism, Gold, Microbial Sensitivity Tests, Biofilms, Candida tropicalis, Metal Nanoparticles
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