Effective cultivation of microalgae for biofuel production: A pilot-scale evaluation of a novel oleaginous microalga Graesiella sp. WBG-1
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BACKGROUND: Commercial production of microalgal biodiesel is not yet economically viable, largely because of low storage lipid yield in microalgae mass cultivation. Selection of lipid-rich microalgae, thus, becomes one of the key research topics for microalgal biodiesel production. However, the laboratory screening protocols alone cannot predict the ability of the strains to dominate and perform in outdoor ponds. Comprehensive assessment of microalgae species should be performed not only under the laboratory conditions, but also in the fields. RESULTS: Laboratory investigations using a bubbled column photobioreactor indicated the microalga Graesiella sp. WBG-1 to be the most productive species among the 63 Chlorophyta strains. In a 10 L reactor, mimicking the industrial circular pond, Graesiella sp. WBG-1 produced 12.03 g biomass m(-2) day(-1) and 5.44 g lipids (45.23 % DW) m(-2) day(-1) under 15 mol m(-2) day(-1) artificial light irradiations. The lipid content decreased to ~34 % DW when the microalga was cultured in 30 L tank PBR under natural solar irradiations, but the decline of lipid content with scaling up was the minimum among the tested strains. Based on these results, the microalga was further tested for its lipid production and culture competitiveness using a pilot-scale raceway pond (200 m(2) illuminated area, culture volume 40,000 L). Consequently, Graesiella sp. WBG-1 maintained a high lipid content (33.4 % DW), of which ~90 % was storage TAGs. Results from the outdoor experiments indicated the nice adaptability of the Graesiella sp. WBG-1 to strong and fluctuating natural solar irradiance and temperature, and also demonstrated several other features, such as large cell size (easy for harvest and resistant to swallow by protozoa) and tolerance to high culture pH (helpful to CO2 fixation). CONCLUSIONS: Graesiella sp. WBG-1 was a promising strain capable of accumulating large amount of storage lipid under nature solar irradiance and temperature. The high lipid content of 33.4 % DW was achieved for the first time in pilot-scale raceway pond. The results also provide evidence for the feasibility of using low-cost raceway pond for autotrophic cultivation of microalgae for biodiesel production.
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Low lipid yield from mass (outdoor) cultivation systems is one of the obstacles to viable commercial production of microalgae biodiesel.
“Low lipid yield from mass (outdoor) cultivation systems is considered as one of the obstacles to the viable commercial production of microalgae biodiesel [41].”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [41]
High lipid accumulation is more difficult to achieve in open ponds.
“High lipid accumulation is more difficult to achieve in open ponds [41].”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [41]
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- Authors
- Wen, X., Du, K., Wang, Z., Peng, X., Luo, L., Tao, H., Xu, Y., Zhang, D., Geng, Y., & Li, Y.
- Affiliations
- Chinese Academy of Sciences (CN)
- Baylor College of Medicine (US)
- Corresponding author
- Li, Yeguang
- Venue
- Biotechnology for Biofuels
- Published
- 2016-06-13
- Volume
- 9
- Issue
- 1
- Pages
- 123-123
- ISSN
- 1754-6834 (ISSN-L)
- PMC
- PMC4906892
- OpenAlex
- W2429150225
- Cited by
- 142
- References
- 41
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 9 · 2023: 15 · 2022: 20 · 2021: 25 · 2020: 16 · 2019: 20 · 2018: 14 · 2017: 8 · 2016: 1
- FWCI
- 3.89
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Biotechnology for Biofuels (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Ministry of Science and Technology of the People's Republic of China (2012FY112900, 2013AA065805)
- China Petrochemical Corporation (210080, 215104)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Keywords
- oleaginous microalgae, microalgae biodiesel, microalgal lipid production, pilot-scale cultivation, Chlorophyta, photobioreactors, strain screening, cell size, pH tolerance, CO2 fixation, autotrophic cultivation
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