The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Effective cultivation of microalgae for biofuel production: a pilot-scale evaluation of a novel oleaginous microalga Graesiella sp. WBG-1
Open accessAbstract
BACKGROUND: Commercial production of microalgal biodiesel is not yet economically viable, largely because of low storage lipid yield in microalgae mass cultivation. Selection of lipid-rich microalgae, thus, becomes one of the key research topics for microalgal biodiesel production. However, the laboratory screening protocols alone cannot predict the ability of the strains to dominate and perform in outdoor ponds. Comprehensive assessment of microalgae species should be performed not only under the laboratory conditions, but also in the fields. RESULTS: Laboratory investigations using a bubbled column photobioreactor indicated the microalga Graesiella sp. WBG-1 to be the most productive species among the 63 Chlorophyta strains. In a 10 L reactor, mimicking the industrial circular pond, Graesiella sp. WBG-1 produced 12.03 g biomass m(-2) day(-1) and 5.44 g lipids (45.23 % DW) m(-2) day(-1) under 15 mol m(-2) day(-1) artificial light irradiations. The lipid content decreased to ~34 % DW when the microalga was cultured in 30 L tank PBR under natural solar irradiations, but the decline of lipid content with scaling up was the minimum among the tested strains. Based on these results, the microalga was further tested for its lipid production and culture competitiveness using a pilot-scale raceway pond (200 m(2) illuminated area, culture volume 40,000 L). Consequently, Graesiella sp. WBG-1 maintained a high lipid content (33.4 % DW), of which ~90 % was storage TAGs. Results from the outdoor experiments indicated the nice adaptability of the Graesiella sp. WBG-1 to strong and fluctuating natural solar irradiance and temperature, and also demonstrated several other features, such as large cell size (easy for harvest and resistant to swallow by protozoa) and tolerance to high culture pH (helpful to CO2 fixation). CONCLUSIONS: Graesiella sp. WBG-1 was a promising strain capable of accumulating large amount of storage lipid under nature solar irradiance and temperature. The high lipid content of 33.4 % DW was achieved for the first time in pilot-scale raceway pond. The results also provide evidence for the feasibility of using low-cost raceway pond for autotrophic cultivation of microalgae for biodiesel production.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Figures
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Low lipid yield from mass (outdoor) cultivation systems is considered as one of the obstacles to the viable commercial production of microalgae biodiesel [41].”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [41]
In the citing paper
“High lipid accumulation is more difficult to achieve in open ponds [41].”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [41]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745
Valuable Products from Biotechnology of Microalgae
Pulz, O., & Gross, W. (2004) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-004-1647-x · Cited by 2,132 · cited 1×
“Background In recent years, mass production of microalgae has been extensively exploited for applications in the industry of food, aquaculture, and bioenergy, and especially for biodiesel production [1, 2].”
BackgroundAn outlook on microalgal biofuels
Wijffels, R. H., & Barbosa, M. J. (2010) · Science · doi:10.1126/science.1189003 · Cited by 1,886
Sheehan, J., Dunahay, T., Benemann, J., & Roessler, P. (1998) · doi:10.2172/15003040 · Cited by 1,753
Lipid productivity as a key characteristic for choosing algal species for biodiesel production
Griffiths, M. J., & Harrison, S. T. L. (2009) · Journal of Applied Phycology · doi:10.1007/s10811-008-9392-7 · Cited by 1,421
Microalgae as a raw material for biofuels production
Gouveia, L., & Oliveira, A. C. (2009) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-008-0495-6 · Cited by 1,284
Shi, Y., Chai, L., Tang, C., Yang, Z., Zhang, H., Chen, R., Chen, Y., & Yu, Z. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-1 · Cited by 1,232
Kumar, A., Ergas, S. J., Yuan, X., Sahu, A., Zhang, Q., Dewulf, J. P., Malcata, F. X., & Langenhove, H. R. V. (2010) · Trends in biotechnology · doi:10.1016/j.tibtech.2010.04.004 · Cited by 752
Marine Polysaccharides from Algae with Potential Biomedical Applications
Raposo, M. F. D. J., Morais, A. M. M. B., & Morais, R. M. S. C. (2015) · Marine Drugs · doi:10.3390/md13052967 · Cited by 712
HANDBOOK OF MICROALGAL CULTURE: BIOTECHNOLOGY AND APPLIED PHYCOLOGY
Henry, E. C. (2004) · Journal of Phycology · doi:10.1111/j.1529-8817.2004.40502.x · Cited by 653
Research works printed but not linked: 3
- Wen XB, Liang F, Geng YH, Li YG. Two-stage characteristics of lipid production in batch culture of two green microalgae. Fresen Environ Bull. 2014;23:2253–8.
- Zhang D, Zhu XY, Wen XB, Geng YH, Li YG. Studies on equilibrium pH values of microalgal medium. Acta Hydrobiol Sin. 2014;3:401–6 (in Chinese with english abstract).
- Li YG, Hu HJ, Gong XM. Studies on the mechanism of pH value change and carbon conversion ratio of Spirulina media. Chin J Biotechnol. 1996;12:242–8 (in Chinese with english abstract).
Cited by
Sorted by citations
Fabris, M., Abbriano, R. M., Pernice, M., Sutherland, D. L., Commault, A. S., Hall, C. C., Labeeuw, L., McCauley, J. I., Kuzhiuparambil, U., Ray, P., et al. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00279 · Cited by 382
Peter, A. P., Koyande, A. K., Chew, K. W., Ho, S., Chen, W., Chang, J., Rambabu, K., BANAT, F. A., & Show, P. L. (2021) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2021.111852 · Cited by 260
Microalgal-based feed: Promising alternative feedstocks for livestock and poultry production
Saadaoui, I., Rasheed, R., Aguilar, A., Cherif, M., Al Jabri, H., Sayadi, S., & Manning, S. R. (2021) · Journal of Animal Science and Biotechnology · doi:10.1186/s40104-021-00593-z · Cited by 223
Microalgae cultivation and harvesting: Growth performance and use of flocculants - A review
Okoro, V., Azimov, U., Munoz, J., Hernández, H. H., & Phan, A. N. (2019) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2019.109364 · Cited by 184
Liu, X.-Y., & Hong, Y. (2021) · Current Pollution Reports · doi:10.1007/s40726-021-00184-6 · Cited by 150
Metadata
- Authors
- Wen, X., Du, K., Wang, Z., Peng, X., Luo, L., Tao, H., Xu, Y., Zhang, D., Geng, Y., & Li, Y.
- Affiliations
- Chinese Academy of Sciences (CN)
- Baylor College of Medicine (US)
- Corresponding author
- Li, Yeguang
- Venue
- Biotechnology for Biofuels
- Published
- 2016-06-13
- Volume
- 9
- Issue
- 1
- Pages
- 123-123
- ISSN
- 1754-6834 (ISSN-L)
- PMC
- PMC4906892
- OpenAlex
- W2429150225
- Cited by
- 142
- References
- 41
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 9 · 2023: 15 · 2022: 20 · 2021: 25 · 2020: 16 · 2019: 20 · 2018: 14 · 2017: 8 · 2016: 1
- FWCI
- 3.89
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Biotechnology for Biofuels (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Ministry of Science and Technology of the People's Republic of China (2012FY112900, 2013AA065805)
- China Petrochemical Corporation (210080, 215104)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Keywords
- oleaginous microalgae, microalgae biodiesel, microalgal lipid production, pilot-scale cultivation, Chlorophyta, photobioreactors, strain screening, cell size, pH tolerance, CO2 fixation, autotrophic cultivation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
