RETRACTED ARTICLE: Dietary garcinol supplementation improves diarrhea and intestinal barrier function associated with its modulation of gut microbiota in weaned piglets
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Abstract Background The effects of dietary garcinol on diarrhea and intestinal barrier function associated with its modulation of gut microbiota in weaned piglets were investigated. Method One hundred forty four weaned piglets (Duroc × Yorkshire × Landrace) from 16 pens (9 piglets per pen) were randomly divided into four treatment groups: controls (CON) or those supplemented with 200 mg/kg (LOW), 400 mg/kg (MID), or 600 mg/kg (HIGH) diet garcinol. After 14-day trial, three piglets per pen were chosen to collect plasma, intestinal tissue and colonic digesta samples. Results We demonstrated for the first time that garcinol promoted growth performance, as increased average daily feed intake (ADFI) and decreased feed/gain ratio (F/G); and reduced diarrhea incidence (P < 0.05); and strengthened antioxidant capacity, as an increased antioxidative index (P < 0.05). Additionally, garcinol ameliorated intestinal barrier dysfunction, as an increased villus height to crypt depth ratio, increased zonula occludens protein 1 (ZO-1), occludin and claudin-1 expression in the jejunum and ileum (P < 0.05), and decreased intestinal permeability (P < 0.05); and reduced inflammation, as decreased cytokine interleukin (IL)-6, IL-10, IL-1β and tumor necrosis factor-α (TNF-α) levels in the mucosa of the jejunum and ileum, and NF-κB p65 translocation (P < 0.05). Moreover, garcinol inhibited the growth of most harmful bacteria in the gut, especially Escherichia coli, and increased the growth of the beneficial bacteria Lactobacillus. Conclusion This work provides a fundamental basis for the future development of garcinol-functional food use for improving diarrhea and intestinal barrier function in weaned piglets and for understanding the biological effects of garcinol and its potential as a functional feed additive.
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Supplementation of dietary garcinol inhibited intestinal barrier dysfunction via elevated ZO-1, CLDN-1, and OCLN expression.
“Supplementation of dietary garcinol inhibited intestinal barrier dysfunction via elevated ZO-1, CLDN-1, and OCLN expression.204”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [204]
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- Authors
- Wang, T., Yao, W., Li, J., Shao, Y., He, Q., Xia, J., & Huang, F.
- Affiliations
- Huazhong Agricultural University (CN)
- Corresponding author
- Huang, Feiruo
- Venue
- Journal of Animal Science and Biotechnology
- Published
- 2020-03-02
- Volume
- 11
- Issue
- 1
- Pages
- 12-12
- ISSN
- 1674-9782 (ISSN-L)
- 2049-1891
- PMC
- PMC7050124
- OpenAlex
- W3012014146
- Cited by
- 51
- References
- 45
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 9 · 2023: 11 · 2022: 9 · 2021: 13 · 2020: 3
- FWCI
- 6.46
- Type
- journal article
- Language
- en
- Status
- Retracted
- Update notices
- retraction (2024-12-02)
- Open access
- gold
- doi.org
- Locations
- Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Key Research and Development Program (2018YFD0500600)
- National Natural Science Foundation of China (31572409)
- Hubei Provincial Natural Science Foundation of China (2018CFA071)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Health Sciences › Veterinary › Small Animals › Veterinary medicine and infectious diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- garcinol, dietary supplementation, weaned piglets, diarrhea, intestinal barrier function, gut microbiota, intestinal permeability, tight junction proteins, intestinal inflammation, NF-κB signaling, Escherichia coli, Lactobacillus, functional feed additives, antioxidant capacity
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