ABCA1 haplodeficiency affects the brain transcriptome following traumatic brain injury in mice expressing human APOE isoforms
Open accessAbstract
Expression of human Apolipoprotein E ( APOE ) modulates the inflammatory response in an isoform specific manner, with APOE4 isoform eliciting a stronger pro-inflammatory response, suggesting a possible mechanism for worse outcome following traumatic brain injury (TBI). APOE lipidation and stability is modulated by ATP-binding cassette transporter A1 (ABCA1), a transmembrane protein that transports lipids and cholesterol onto APOE. We examined the impact of Abca1 deficiency and APOE isoform expression on the response to TBI using 3-months-old, human APOE3 +/ + ( E3/Abca1 +/+ ) and APOE4 +/+ ( E4/Abca1 +/+ ) targeted replacement mice, and APOE3 + /+ and APOE4 +/+ mice with only one functional copy of the Abca1 gene ( E3/Abca1 +/− ; E4/Abca1 +/− ). TBI-treated mice received a craniotomy followed by a controlled cortical impact (CCI) brain injury in the left hemisphere; sham-treated mice received the same surgical procedure without the impact. We performed RNA-seq using samples from cortices and hippocampi followed by genome-wide differential gene expression analysis. We found that TBI significantly impacted unique transcripts within each group, however, the proportion of unique transcripts was highest in E4/Abca1 +/− mice. Additionally, we found that Abca1 haplodeficiency increased the expression of microglia sensome genes among only APOE4 injured mice, a response not seen in injured APOE3 mice, nor in either group of sham-treated mice. To identify gene networks, or modules, correlated to TBI, APOE isoform and Abca1 haplodeficiency, we used weighted gene co-expression network analysis (WGCNA). The module that positively correlated to TBI groups was associated with immune response and featured hub genes that were microglia-specific, including Trem2, Tyrobp, Cd68 and Hexb. The modules positively correlated with APOE4 isoform and negatively to Abca1 haplodeficient mice represented “protein translation” and “oxidation-reduction process”, respectively. Our results reveal E4/Abca1 +/− TBI mice have a distinct response to injury, and unique gene networks are associated with APOE isoform, Abca1 insufficiency and injury.
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BNIP3 encodes BCL2 interacting protein 3, which is increased in hypoxic-ischemic-encephalopathy and physiological ageing, and increases in association with cellular senescence especially in cells over-expressing p21 and p16.
“BNIP3 (FC = 1.17, p = 0.04) encodes BCL2 interacting protein 3, which is increased in hypoxic-ischemic-encephalopathy [18] and physiological ageing [31], and has been shown to increase in association with cellular senescence especially in cells over-expressing p21 and p16 [16].”
From Early onset senescence and cognitive impairment in a murine model of repeated mTBI (2021) · cites this paper as [18]
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Cites
Moderate and severe traumatic brain injury in adults
Maas, A. I., Stocchetti, N., & Bullock, R. (2008) · The Lancet Neurology · doi:10.1016/s1474-4422(08)70164-9 · Cited by 2,123 · see citing works
Mutations in ABC1 in Tangier disease and familial high-density lipoprotein deficiency
Brooks-Wilson, A., Marcil, M., Clee, S. M., Zhang, L.-H., Roomp, K., van Dam, M., Yu, L., Brewer, C., Collins, J. A., Molhuizen, H. O. F., et al. (1999) · Nature Genetics · doi:10.1038/11905 · Cited by 1,687 · see citing works
The role of apolipoprotein E in Alzheimer's disease
Kim, J., Basak, J. M., & Holtzman, D. M. (2009) · Neuron · doi:10.1016/j.neuron.2009.06.026 · Cited by 1,661 · see citing works
The far-reaching scope of neuroinflammation after traumatic brain injury
Simon, D. W., McGeachy, M. J., Bayır, H., Clark, R. S. B., Loane, D. J., & Kochanek, P. M. (2017) · Nature Reviews Neurology · doi:10.1038/nrneurol.2017.13 · Cited by 1,190 · cited 1× · see citing works
“constitute the primary injury, whereas the secondary injury occurs after the inciting traumatic event, and involves multiple pathways and signaling cascades that can cause further damage [3, 13, 39].”
Microglial activation by Alzheimer amyloid precursor protein and modulation by apolipoprotein E
Barger, S. W., & Harmon, A. D. (1997) · Nature · doi:10.1038/42257 · Cited by 622 · see citing works
Epidemiology of traumatic brain injury
Faul, M., & Coronado, V. G. (2015) · Handbook of clinical neurology · doi:10.1016/b978-0-444-52892-6.00001-5 · Cited by 514 · see citing works
Trushina, E., Dutta, T., Persson, X.-M. T., Mielke, M. M., & Petersen, R. C. (2013) · PLoS ONE · doi:10.1371/journal.pone.0063644 · Cited by 438 · see citing works
Weighted Gene Coexpression Network Analysis: State of the Art
Zhao, W., Langfelder, P., Fuller, T. F., Dong, J., Li, A., & Hovarth, S. (2010) · Journal of Biopharmaceutical Statistics · doi:10.1080/10543400903572753 · Cited by 393 · see citing works
Cognitive functioning ten years following traumatic brain injury and rehabilitation.
Draper, K., & Ponsford, J. (2008) · Neuropsychology · doi:10.1037/0894-4105.22.5.618 · Cited by 380 · see citing works
Rapid Erasure of Long-Term Memory Associations in the Cortex by an Inhibitor of PKMζ
Shema, R., Sacktor, T. C., & Dudai, Y. (2007) · Science · doi:10.1126/science.1144334 · Cited by 353 · see citing works
Lack of ABCA1 Considerably Decreases Brain ApoE Level and Increases Amyloid Deposition in APP23 Mice
Koldamova, R., Staufenbiel, M., & Lefterov, I. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m504513200 · Cited by 350 · see citing works
Self-citationMcMillan, T. M., Teasdale, G. M., & Stewart, E. (2012) · Journal of Neurology, Neurosurgery & Psychiatry · doi:10.1136/jnnp-2012-302746 · Cited by 315 · see citing works
New perspectives on central and peripheral immune responses to acute traumatic brain injury
Das, M., Mohapatra, S., & Mohapatra, S. S. (2012) · Journal of Neuroinflammation · doi:10.1186/1742-2094-9-236 · Cited by 282 · see citing works
Pathania, D., Millard, M., & Neamati, N. (2009) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2009.05.010 · Cited by 265 · see citing works
Morganti, J. M., Jopson, T. D., Liu, S., Riparip, L.-K., Guandique, C. K., Gupta, N., Ferguson, A. R., & Rosi, S. (2015) · The Journal of Neuroscience · doi:10.1523/jneurosci.2405-14.2015 · Cited by 253 · cited 1× · see citing works
“mation and inflammatory molecules are strongly correlated with multiple measures of outcome in patients, including neurobehavioral impairments and survival rates [29, 45].”
Whitnall, L. (2006) · Journal of Neurology Neurosurgery & Psychiatry · doi:10.1136/jnnp.2005.078246 · Cited by 252 · see citing works
Müller, W. E., Eckert, A., Kurz, C., Eckert, G. P., & Leuner, K. (2010) · Molecular Neurobiology · doi:10.1007/s12035-010-8141-5 · Cited by 243 · see citing works
APOE genotype influences acquisition and recall following traumatic brain injury
Crawford, F., Vanderploeg, R. D., Freeman, M. J., Singh, S., Waisman, M., Michaels, L., Abdullah, L. I., Warden, D. L., Lipsky, R. H., Salazar, A., et al. (2002) · Neurology · doi:10.1212/wnl.58.7.1115 · Cited by 207 · see citing works
Acosta, S. A., Tajiri, N., Shinozuka, K., Ishikawa, H., Grimmig, B. A., Diamond, D. M., Sanberg, P. R., Bickford, P. C., Kaneko, Y., & Borlongan, C. V. (2013) · PLoS ONE · doi:10.1371/journal.pone.0053376 · Cited by 194 · see citing works
Long-term Neurologic Outcomes After Traumatic Brain Injury
Bazarian, J. J., Černak, I., Noble‐Haeusslein, L. J., Potolicchio, S. J., & Temkin, N. R. (2009) · Journal of Head Trauma Rehabilitation · doi:10.1097/htr.0b013e3181c15600 · Cited by 190 · see citing works
Lynch, J., Morgan, D., Mance, J., Matthew, W. D., & Laskowitz, D. T. (2001) · Journal of Neuroimmunology · doi:10.1016/s0165-5728(00)00459-8 · Cited by 188 · see citing works
Increased Risk of Late Posttraumatic Seizures Associated With Inheritance of APOE ϵ4 Allele
Diaz-Arrastia, R. R., Gong, Y., Fair, S., Scott, K. D., García, M. C., Carlile, M. C., Agostini, M. A., & Ness, P. C. V. (2003) · Archives of Neurology · doi:10.1001/archneur.60.6.818 · Cited by 186 · see citing works
ATP-binding cassette transporter A1: From metabolism to neurodegeneration
Koldamova, R., Fitz, N. F., & Lefterov, I. (2014) · Neurobiology of Disease · doi:10.1016/j.nbd.2014.05.007 · Cited by 144 · cited 1× · see citing works
“ABCA1 is a major regulator of cholesterol transport and an essential mediator of high density lipoprotein generation [22].”
Self-citationSix-month recovery from mild to moderate Traumatic Brain Injury: the role of APOE- 4 allele
Chamelian, L. (2004) · Brain · doi:10.1093/brain/awh296 · Cited by 140 · see citing works
Fitz, N. F., Cronican, A. A., Saleem, M., Fauq, A. H., Chapman, R., Lefterov, I. M., & Koldamova, R. P. (2012) · Journal of Neuroscience · doi:10.1523/jneurosci.1937-12.2012 · Cited by 138 · see citing works
Self-citationτ Proteins in Serum Predict Outcome After Severe Traumatic Brain Injury
Liliang, P., Liang, C.-L., Weng, H., Lu, K., Wang, K., Chen, H., & Chuang, J. (2009) · Journal of Surgical Research · doi:10.1016/j.jss.2008.12.022 · Cited by 137 · see citing works
Therapies targeting lipid peroxidation in traumatic brain injury
Anthonymuthu, T. S., Kenny, E., & Bayır, H. (2016) · Brain Research · doi:10.1016/j.brainres.2016.02.006 · Cited by 137 · see citing works
CDC Grand Rounds: Reducing Severe Traumatic Brain Injury in the United States
Wright, D. W., Kellermann, A. L., McGuire, L., Chen, B., & Popović, T. (2013) · PubMed Central · Cited by 136 · see citing works
Yang, S. H., Gangidine, M. M., Pritts, T. A., Goodman, M. D., & Lentsch, A. B. (2013) · Shock · doi:10.1097/shk.0000000000000037 · Cited by 133 · see citing works
ABCA1 influences neuroinflammation and neuronal death
Karasinska, J. M., de Haan, W., Franciosi, S., Ruddle, P., Fan, J., Kruit, J. K., Stukas, S., Lütjohann, D., Gutmann, D. H., Wellington, C. L., et al. (2013) · Neurobiology of Disease · doi:10.1016/j.nbd.2013.01.018 · Cited by 104 · see citing works
Apolipoprotein E4 Allele Presence and Functional Outcome after Severe Traumatic Brain Injury
Alexander, S. A., Kerr, M. E., Kim, Y., Kamboh, M. I., Beers, S. R., & Conley, Y. P. (2007) · Journal of Neurotrauma · doi:10.1089/neu.2006.0133 · Cited by 96 · see citing works
Effect of high fat diet on phenotype, brain transcriptome and lipidome in Alzheimer’s model mice
Nam, K. N., Mounier, A., Wolfe, C. M., Fitz, N. F., Carter, A. Y., Castranio, E. L., Kamboh, H., Reeves, V. L., Wang, J., Han, X., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-04412-2 · Cited by 94 · see citing works
Self-citationCDC grand rounds: reducing severe traumatic brain injury in the United States.
(2013) · PubMed · Cited by 89 · see citing works
Laskowitz, D. T., Fillit, H. M., Yeung, N., Toku, K., & Vitek, M. P. (2006) · Acta Neurologica Scandinavica · doi:10.1111/j.1600-0404.2006.00680.x · Cited by 82 · see citing works
Role for the pleckstrin homology domain‐containing protein CKIP‐1 in AP‐1 regulation and apoptosis
Zhang, L., Xing, G., Tie, Y., Tang, Y., Tian, C., Li, L., Sun, L., Wei, H., Zhu, Y., & He, F. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600532 · Cited by 78 · see citing works
Mannix, R., Zhang, J., Park, J., Zhang, X., Bilal, K., Walker, K., Tanzi, R. E., Tesco, G., & Whalen, M. J. (2010) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.2010.99 · Cited by 63 · see citing works
Chronic post-traumatic stress disorder-related traits in a rat model of low-level blast exposure
Perez-Garcia, G., Sosa, M. A. G., Gasperi, R. D., Lashof-Sullivan, M. M., Maudlin-Jeronimo, E., Stone, J. R., Haghighi, F. G., Ahlers, S. T., & Elder, G. A. (2016) · Behavioural Brain Research · doi:10.1016/j.bbr.2016.09.061 · Cited by 59 · see citing works
High-Density Lipoprotein Regulation of Mitochondrial Function
White, C. R., Datta, G., & Giordano, S. S. (2017) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-55330-6_22 · Cited by 58 · see citing works
Castranio, E. L., Mounier, A., Wolfe, C. M., Nam, K. N., Fitz, N. F., Letronne, F., Schug, J., Koldamova, R. P., & Lefterov, I. M. (2017) · Neurobiology of Disease · doi:10.1016/j.nbd.2017.05.006 · Cited by 55 · see citing works
Self-citationLi, X., Montine, K. S., Keene, C. D., & Montine, T. J. (2015) · The FASEB Journal · doi:10.1096/fj.14-262683 · Cited by 54 · see citing works
Wang, S., Sheng, T., Ren, S., Tian, T., & Lü, W. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.07.030 · Cited by 52 · see citing works
LAMP5 Fine-Tunes GABAergic Synaptic Transmission in Defined Circuits of the Mouse Brain
Tiveron, M.-C., Beurrier, C., Ceni, C., Andriambao, N., Combes, A. J., Koehl, M., Maurice, N., Gatti, E., Abrous, D. N., Goff, L. K., et al. (2016) · PLoS ONE · doi:10.1371/journal.pone.0157052 · Cited by 46 · see citing works
Blocquel, D., Li, S., Wei, N., Daub, H., Sajish, M., Erfurth, M., Kooi, G., Zhou, J., Bai, G., SCHIMMEL, P. R., et al. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx455 · Cited by 44 · see citing works
Traumatic brain injury and late-life dementia
Plassman, B. L., & Grafman, J. H. (2015) · Handbook of clinical neurology · doi:10.1016/b978-0-444-63521-1.00044-3 · Cited by 40 · see citing works
Yu, H., Lin, X., Wang, D., Zhang, Z., Guo, Y. M., Ren, X., Xu, B., Yuan, J., Liu, J., Spencer, P. S., et al. (2018) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2018.00074 · Cited by 40 · see citing works
Nam, K. N., Mounier, A., Fitz, N. F., Wolfe, C. M., Schug, J., Lefterov, I. M., & Koldamova, R. P. (2016) · Scientific Reports · doi:10.1038/srep24048 · Cited by 36 · see citing works
Self-citationIntegrated approach reveals diet, APOE genotype and sex affect immune response in APP mice
Nam, K. N., Wolfe, C. M., Fitz, N. F., Letronne, F., Castranio, E. L., Mounier, A., Schug, J., Lefterov, I., & Koldamova, R. (2018) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2017.10.018 · Cited by 32 · see citing works
Self-citationImpact of apolipoprotein gene on cognitive impairment and recovery after traumatic brain injury
Ponsford, J. L., Rudzki, D., Bailey, K. A., & Ng, K. T. (2007) · Neurology · doi:10.1212/01.wnl.0000254609.04330.9d · Cited by 31 · see citing works
Carter, A. Y., Letronne, F., Fitz, N. F., Mounier, A., Wolfe, C. M., Nam, K. N., Reeves, V. L., Kamboh, H., Lefterov, I. M., & Koldamova, R. P. (2017) · PLoS ONE · doi:10.1371/journal.pone.0172161 · Cited by 25 · see citing works
Self-citationGhroubi, S., Feki, I., Chelly, H., & Elleuch, M. H. (2016) · Annals of Physical and Rehabilitation Medicine · doi:10.1016/j.rehab.2016.07.302 · Cited by 4 · see citing works
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Jacobo‐Albavera, L., Domínguez‐Pérez, M., Medina-Leyte, D. J., González-Garrido, A., & Villarreal-Molina, M. T. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22041593 · Cited by 163 · see citing works
Chen, Y., Meng, J., Xu, Q., Long, T., Bi, F., Chang, C., & Liu, W. (2019) · Brain Research · doi:10.1016/j.brainres.2019.01.005 · Cited by 94 · see citing works
Wang, M., Wang, L., Pu, L., Li, K., Feng, T., Zheng, P., Li, S., Sun, M., Yao, Y., & Jin, L. (2020) · Genomics · doi:10.1016/j.ygeno.2020.01.001 · Cited by 91 · see citing works
Elder, G. A., Ehrlich, M. E., & Gandy, S. (2019) · Neuroscience Letters · doi:10.1016/j.neulet.2019.134294 · Cited by 73 · see citing works
Lewandowski, C. T., Maldonado Weng, J., & LaDu, M. J. (2020) · Neurobiology of Disease · doi:10.1016/j.nbd.2020.104811 · Cited by 72 · see citing works
Sloley, S. S., Main, B. S., Winston, C. N., Harvey, A. C., Kaganovich, A., Korthas, H. T., Caccavano, A., Zapple, D. N., Wu, J., Partridge, J. G., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-22744-6 · Cited by 62 · see citing works
Early onset senescence and cognitive impairment in a murine model of repeated mTBI
Schwab, N., Ju, Y., & Hazrati, L.-N. (2021) · Acta Neuropathologica Communications · doi:10.1186/s40478-021-01190-x · Cited by 39 · see citing works
The role of the ATP-Binding Cassette A1 (ABCA1) in neurological disorders: a mechanistic review
Paseban, T., Alavi, M. S., Etemad, L., & Roohbakhsh, A. (2023) · Expert opinion on therapeutic targets · doi:10.1080/14728222.2023.2235718 · Cited by 26 · see citing works
Xu, H., Zheng, L., Chen, X., Pang, Q., Yan, Y., Liu, R., Guo, H., Ren, Z., Yang, Y., Gu, Z., et al. (2022) · Brain Pathology · doi:10.1111/bpa.13126 · Cited by 21 · see citing works
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Self-citationZhao, J., Xu, C., Cao, H., Zhang, L., Wang, X., & Chen, S. (2019) · PeerJ · doi:10.7717/peerj.8324 · Cited by 20 · see citing works
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- Authors
- Castranio, E. L., Wolfe, C. M., Nam, K. N., Letronne, F., Fitz, N. F., Lefterov, I., & Koldamova, R.
- Affiliations
- University of Pittsburgh (US)
- Corresponding author
- Lefterov, Iliya
- Koldamova, Radosveta
- ORCID
- Venue
- Acta Neuropathologica Communications
- Published
- 2018-07-26
- Volume
- 6
- Issue
- 1
- Pages
- 69-69
- ISSN
- 2051-5960 (ISSN-L)
- PMC
- PMC6062955
- OpenAlex
- W2884483584
- Cited by
- 23
- References
- 50
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 3 · 2022: 3 · 2021: 5 · 2020: 2 · 2019: 8
- FWCI
- 2.55
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Acta Neuropathologica Communications (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- U.S. Department of Defense (W81XWH-13-1-0384)
- National Institute on Aging (K01AG044490, AG056371, AG057565)
- National Institute of Environmental Health Sciences (ES024233)
- Topics
- Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Health Sciences › Medicine › Epidemiology › Traumatic Brain Injury Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- traumatic brain injury, apolipoprotein E, APOE ε4, microglia, transcriptome, controlled cortical impact, gene co-expression network, TREM2, TYROBP, CD68, HEXB, inflammation, lipid modification, targeted replacement mice
- MeSH terms
- Brain Injuries, Traumatic, Animals, Apolipoproteins E, Brain, Disease Models, Animal, Gene Expression Regulation, Humans, Mice, Inbred C57BL, Mice, Transgenic, RNA, Messenger, Microglia, Protein Isoforms, Mice, Gene Regulatory Networks, ATP Binding Cassette Transporter 1
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