Cited by 373D Printing in Biomedical ResearchGut microbiota and healthTissue Engineering and Regenerative Medicine

Effect of gut microbiome-derived metabolites and extracellular vesicles on hepatocyte functions in a gut-liver axis chip

Open access
Kang, Seong Goo, Choi, Yoon Young, Mo, Sung Jun, Kim, Tae Hyeon, Ha, Jang Ho, Hong, Dong Ki, Lee, Hayera, Park, Soo Dong +3 more·2023·Nano Convergence

Abstract

Metabolism, is a complex process involving the gut and the liver tissue, is difficult to be reproduced in vitro with conventional single cell culture systems. To tackle this challenge, we developed a gut-liver-axis chip consisting of the gut epithelial cell chamber and three-dimensional (3D) uniform-sized liver spheroid chamber. Two cell culture chamber compartments were separated with a porous membrane to prevent microorganisms from passing through the chamber. When the hepG2 spheroids cultured with microbiota-derived metabolites, we observed the changes in the physiological function of hepG2 spheroids, showing that the albumin and urea secretion activity of liver spheroids was significantly enhanced. Additionally, the functional validation of hepG2 spheroids treated with microbiota-derived exosome was evaluated that the treatment of the microbiota-derived exosome significantly enhanced albumin and urea in hepG2 spheroids in a gut-liver axis chip. Therefore, this gut-liver axis chip could be a potentially powerful co-culture platform to study the interaction of microbiota and host cells. Graphical Abstract

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  • EVs are proficient at carrying diverse biomolecules, such as proteins, mRNAs, and non-coding RNAs, to target cells.

    “EVs are proficient at carrying diverse biomolecules, such as proteins, messenger RNAs (mRNAs), and non-coding RNAs, to target cells [45–47].”

    From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [46]

    The Introduction says EVs carry proteins and nucleic acids and transfer genetic material to target cells. It does not identify mRNAs or non-coding RNAs specifically, so those examples are not verified by this source. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

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1 of the 50 references couldn’t be matched to a known paper.

Research works printed but not linked: 4

  1. [1]Bein A Shin W Jalili-Firoozinezhad S Park MH Sontheimer-Phelps A Tovaglieri A Chalkiadaki A Kim HJ Ingber DE Cell Mol. Gastroenter. 2018 5 659 PMC5924739 29713674
  2. [14]Liu J Tan Y Cheng H Zhang D Feng W Peng C Aging Dis. 2022 13 10.14336 PMC9286904 35855347
  3. [22]L. Tomasova, M. Grman, K. Ondrias, and M. Ufnal, Nutr. Metab. 18 (2021). PMC8281717 34266472
  4. [37]M. Chávarri, L. Diez-Gutiérrez, I. Marañón, and L. J. R. Barron, in Advances in Probiotics (Elsevier, 2021), pp. 259.

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Authors
Kang, S. G., Choi, Y. Y., Mo, S. J., Kim, T. H., Ha, J. H., Hong, D. K., Lee, H., Park, S. D., Shim, J.-J., Lee, J.-L., & Chung, B. G.
Affiliations
Sogang University (KR)
Corresponding author
Chung, Bong Geun
Venue
Nano Convergence
Published
2023-01-16
Volume
10
Issue
1
Pages
5-5
ISSN
2196-5404 (ISSN-L)
OpenAlex
W4316499831
Cited by
37
References
50
Citations by year
2026: 10 · 2025: 11 · 2024: 15 · 2023: 1
FWCI
2.92
Type
journal article
Language
en
Open access
Locations
  • Nano Convergence (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
hy Co., Ltd. (2022-00035)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
gut-liver axis, gut microbiota metabolites, extracellular vesicles, exosomes, hepatocyte function, liver spheroids, HepG2 cells, intestinal epithelial cells, albumin secretion, urea secretion, microbiota-host interactions

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