Effects of Water-Soluble Antioxidant from Spinach, NAO, on Doxorubicin-Induced Heart Injury
Abstract
Doxorubicin (DOX) produces clinically restorative responses in numerous human cancers, but its cardiotoxicity has limited its usefulness. Because reactive oxygen species may affect DOX-induced antitumor activity and cardiotoxicity, we evaluated the prophylactic effect of spinach natural antioxidant (NAO) on DOX-induced cardiotoxicity and oxidative stress in female Balb/c mice using histological, electron microscopical and biochemical parameters. Mice were treated with NAO for 7 days prior to and/or for 6 days after DOX administration. Pretreatment with NAO (cumulative dose: 130 mg/kg) did not hinder the effectiveness of DOX. Light and electron microscopy of DOX-treated heart revealed myocardial degeneration. When administered combined before and after DOX, NAO conferred the most significant cardiac protection. The effects of NAO on the lipid peroxidation product, malondialdehyde, and on H2O2/ hydroperoxides were examined on day 6 following DOX administration; levels of both were elevated in DOX-treated mice, compared to control. Pretreatment with NAO prevented these changes. Pretreatment with NAO before DOX administration decreased catalase and increased superoxide dismutase activities compared to the DOX group. Our results suggest usage of NAO in combination with DOX as a prophylactic strategy to protect heart muscle from DOX-induced cellular damage.
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Spinach is associated with numerous health advantages, such as antioxidant characteristics, lipid-lowering effects, appetite regulation, tissue regeneration, and protection against oxidative stress-induced damage.
“These results show that spinach is associated with numerous health advantages, such as antioxidant characteristics, lipid-lowering effects, appetite regulation, tissue regeneration, and protection against oxidative stress-induced damage [156,162,164,165,166,167,168,169,170].”
From Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition (2024) · cites this paper as [170]
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ENVIRONMENTAL SOLUTIONS GROUP, LLC
Oshita, C. (2011)
- Authors
- Breitbart, E., Lomnitski, L., Nyska, A., Malik, Z., Bergman, M., Sofer, Y., Haseman, J. K., & Grossman, S.
- Affiliations
- Bar-Ilan University (IL)
- National Institute of Environmental Health Sciences (US)
- Ariel University (IL)
- ORCID
- Nyska, A
- Venue
- Human & Experimental Toxicology
- Publisher
- SAGE Publications
- Published
- 2001-07
- Volume
- 20
- Issue
- 7
- Pages
- 337-345
- ISSN
- 0960-3271 (ISSN-L)
- 1477-0903
- OpenAlex
- W2157864692
- Cited by
- 31
- References
- 45
- Citations by year
- 2024: 1 · 2023: 2 · 2021: 1 · 2018: 2 · 2017: 2 · 2014: 1 · 2013: 3
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Human & Experimental Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Environmental Health Sciences
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Chemotherapy-induced cardiotoxicity and mitigation
- Health Sciences › Medicine › Pathology and Forensic Medicine › Chemotherapy-induced organ toxicity mitigation
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Bioactive Compounds and Antitumor Agents
- Keywords
- doxorubicin, cardiotoxicity, spinach, natural antioxidants, oxidative stress, reactive oxygen species, lipid peroxidation, malondialdehyde, hydrogen peroxide, superoxide dismutase, catalase, cardioprotection, myocardial degeneration, chemoprevention
- MeSH terms
- Animals, Antineoplastic Agents, Antioxidants, Doxorubicin, Female, Heart Diseases, Mice, Inbred BALB C, Microscopy, Electron, Myocardium, Plant Extracts, Solubility, Lipid Peroxidation, Reactive Oxygen Species, Oxidative Stress, Spinacia oleracea, Mice
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