Simultaneous quantification of cholesterol sulfate, androgen sulfates, and progestagen sulfates in human serum by LC-MS/MS
Open accessAbstract
Steroids are primarily present in human fluids in their sulfated forms. Profiling of these compounds is important from both diagnostic and physiological points of view. Here, we present a novel method for the quantification of 11 intact steroid sulfates in human serum by LC-MS/MS. The compounds analyzed in our method, some of which are quantified for the first time in blood, include cholesterol sulfate, pregnenolone sulfate, 17-hydroxy-pregnenolone sulfate, 16-α-hydroxy-dehydroepiandrosterone sulfate, dehydroepiandrosterone sulfate, androstenediol sulfate, androsterone sulfate, epiandrosterone sulfate, testosterone sulfate, epitestosterone sulfate, and dihydrotestosterone sulfate. The assay was conceived to quantify sulfated steroids in a broad range of concentrations, requiring only 300 μl of serum. The method has been validated and its performance was studied at three quality controls, selected for each compound according to its physiological concentration. The assay showed good linearity (R(2) > 0.99) and recovery for all the compounds, with limits of quantification ranging between 1 and 80 ng/ml. Averaged intra-day and between-day precisions (coefficient of variation) and accuracies (relative errors) were below 10%. The method has been successfully applied to study the sulfated steroidome in diseases such as steroid sulfatase deficiency, proving its diagnostic value. This is, to our best knowledge, the most comprehensive method available for the quantification of sulfated steroids in human blood.
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16α-hydroxy-DHEAS, 5-diol-sulfate, AST-sulfate, and EpiAST-sulfate can be measured in nM concentrations in serum of both men and women.
“16α-hydroxy-DHEAS, 5-diol-sulfate, AST-sulfate, EpiAST-sulfate can be measured in nM concentrations in serum of both men and women (Labrie et al., 1997b, Sanchez-Guijo et al., 2015, Zang et al., 2017).”
From Intracrine androgen biosynthesis, metabolism and action revisited (2018) · cites this paper as Sanchez-Guijo et al., 2015
Diagnosis of STS deficiency can readily be achieved by UHPLC-MS/MS analysis of the intact conjugate.
“Serum cholesterol sulfate is highly elevated in STS deficiency (79), and diagnosis can readily be achieved by UHPLC-MS/MS analysis of the intact conjugate (80, 81).”
From Steroid Metabolome Analysis in Disorders of Adrenal Steroid Biosynthesis and Metabolism (2019) · cites this paper as [81]
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Steroid Metabolome Analysis in Disorders of Adrenal Steroid Biosynthesis and Metabolism
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- Authors
- Sánchez-Guijo, A., Oji, V., Hartmann, M. F., Traupe, H., & Wudy, S. A.
- Affiliations
- University of Münster (DE)
- Justus-Liebig-Universität Gießen (DE)
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 2015-09
- Volume
- 56
- Issue
- 9
- Pages
- 1843-1851
- ISSN
- 0022-2275 (ISSN-L)
- PMC
- PMC4548788
- OpenAlex
- W2142627757
- Cited by
- 73
- References
- 20
- Citations by year
- 2025: 4 · 2024: 4 · 2023: 2 · 2022: 5 · 2021: 9 · 2020: 8 · 2019: 11 · 2018: 8 · 2017: 10 · 2016: 11 · 2015: 1
- FWCI
- 4.34
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Keywords
- steroid sulfates, cholesterol sulfate, human serum, pregnenolone sulfate, dehydroepiandrosterone sulfate, steroid sulfatase deficiency, steroid quantification
- MeSH terms
- Cholesterol Esters, Chromatography, Liquid, Humans, Progestins, Sulfates, Testosterone Congeners, Tandem Mass Spectrometry
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