The DOI matches its Crossref record.
Circulating DNA and DNase Activity in Human Blood
Abstract
Abstract: The concentration of circulating DNA (cirDNA) and deoxyribonuclease activity in blood plasma of healthy donors and patients with colon or stomach cancer were analyzed. The concentration of DNA was measured using Hoechst 33258 fluorescent assay after the isolation by the glass–milk protocol. A 1‐kbp PCR product labeled with biotinylated forward and fluorescein‐labeled reverse primers was used as a substrate for DNase. DNase activity was estimated from the data of immunochemical detection of the nonhydrolyzed amplicon. The average concentration of cirDNA in the plasma of healthy donors was low (34 ± 34 ng/mL), and was accompanied with high DNase activity (0.356 ± 0.410 U/mL). The increased concentrations of cirDNA in blood plasma of patients with colon and stomach cancer were accompanied by a decrease in DNase activity below the detection level of the assay. The data obtained demonstrate that low DNase activity in blood plasma of cancer patients can cause an increase in the concentration of cirDNA.
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Cited by
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Kustanovich, A., Schwartz, R., Peretz, T., & Grinshpun, A. (2019) · Cancer Biology & Therapy · doi:10.1080/15384047.2019.1598759 · Cited by 606
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Bronkhorst, A. J., Ungerer, V., & Holdenrieder, S. (2019) · Biomolecular Detection and Quantification · doi:10.1016/j.bdq.2019.100087 · Cited by 566
Dyrskjøt, L., Hansel, D. E., Efstathiou, J. A., Knowles, M. A., Galsky, M. D., Teoh, J., & Theodorescu, D. (2023) · Nature Reviews Disease Primers · doi:10.1038/s41572-023-00468-9 · Cited by 544
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Metadata
- Authors
- TAMKOVICH, S. N., CHEREPANOVA, A. V., KOLESNIKOVA, E. V., RYKOVA, E. Y., PYSHNYI, D. V., VLASSOV, V. V., & LAKTIONOV, P. P.
- Venue
- Annals of the New York Academy of Sciences
- Publisher
- Wiley
- Published
- 2006-09
- Volume
- 1075
- Issue
- 1
- Pages
- 191-196
- ISSN
- 0077-8923 (ISSN-L)
- 1749-6632
- OpenAlex
- W2086261989
- Cited by
- 232
- References
- 0
- FWCI
- 2.40
- Type
- journal article
- Language
- en
- Open access
- closed
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- TAMKOVICH, S. N., CHEREPANOVA, A. V., KOLESNIKOVA, E. V., RYKOVA, E. Y., PYSHNYI, D. V., VLASSOV, V. V., & LAKTIONOV, P. P. (2006). Circulating DNA and DNase Activity in Human Blood. Annals of the New York Academy of Sciences. https://doi.org/10.1196/annals.1368.026
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