Empirical Bayes analysis of a microarray experiment
Abstract
Microarrays are a novel technology that facilitates the simultaneous measurement of thousands of gene expression levels. A typical microarray experiment can produce millions of data points, raising serious problems of data reduction, and simultaneous inference. We consider one such experiment in which oligonucleotide arrays were employed to assess the genetic effects of ionizing radiation on seven thousand human genes. A simple nonparametric empirical Bayes model is introduced, which is used to guide the efficient reduction of the data to a single summary statistic per gene, and also to make simultaneous inferences concerning which genes were affected by the radiation. Although our focus is on one specific experiment, the proposed methods can be applied quite generally. The empirical Bayes inferences are closely related to the frequentist false discovery rate (FDR) criterion.
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The approach is sound and the local posterior corresponds to the local positive predictive value for a computational tool, an equivalent of the local false discovery rate.
“This approach is sound and the local posterior corresponds to the local positive predictive value for a computational tool, an equivalent of the local false discovery rate.36,37”
From Calibration of computational tools for missense variant pathogenicity classification and ClinGen recommendations for PP3/BP4 criteria (2022) · cites this paper as [36]
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- Authors
- Efron, B., Tibshirani, R., Storey, J. D., & Tusher, V.
- Affiliations
- Stanford University (US)
- Venue
- Journal of the American Statistical Association
- Publisher
- Informa UK Limited
- Published
- 2001-12
- Volume
- 96
- Issue
- 456
- Pages
- 1151-1160
- ISSN
- 0162-1459 (ISSN-L)
- 1537-274X
- OpenAlex
- W2074089196
- Cited by
- 1,733
- References
- 12
- Citations by year
- 2026: 17 · 2025: 34 · 2024: 39 · 2023: 49 · 2022: 46 · 2021: 72 · 2020: 43 · 2019: 51 · 2018: 61 · 2017: 55 · 2016: 47 · 2015: 66 · 2014: 82 · 2013: 74 · 2012: 112
- FWCI
- 17.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the American Statistical Association (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
- Physical Sciences › Mathematics › Statistics and Probability › Statistical Methods and Inference
- Keywords
- empirical Bayes, microarray data, gene expression, oligonucleotide microarrays, ionizing radiation, data reduction, simultaneous inference, false discovery rate, gene selection
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