Cited by 1,733Gene expression and cancer classificationBioinformatics and Genomic NetworksStatistical Methods and Inference

Empirical Bayes analysis of a microarray experiment

Efron, Bradley, Tibshirani, Robert, Storey, John D, Tusher, Virginia·2001·Journal of the American Statistical Association

Abstract

Microarrays are a novel technology that facilitates the simultaneous measurement of thousands of gene expression levels. A typical microarray experiment can produce millions of data points, raising serious problems of data reduction, and simultaneous inference. We consider one such experiment in which oligonucleotide arrays were employed to assess the genetic effects of ionizing radiation on seven thousand human genes. A simple nonparametric empirical Bayes model is introduced, which is used to guide the efficient reduction of the data to a single summary statistic per gene, and also to make simultaneous inferences concerning which genes were affected by the radiation. Although our focus is on one specific experiment, the proposed methods can be applied quite generally. The empirical Bayes inferences are closely related to the frequentist false discovery rate (FDR) criterion.

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Crossref · 10.1198/016214501753382129

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Authors
Efron, B., Tibshirani, R., Storey, J. D., & Tusher, V.
Affiliations
Stanford University (US)
Venue
Journal of the American Statistical Association
Published
2001-12
Volume
96
Issue
456
Pages
1151-1160
ISSN
  • 0162-1459 (ISSN-L)
  • 1537-274X
OpenAlex
W2074089196
Cited by
1,733
References
12
Citations by year
2026: 17 · 2025: 34 · 2024: 39 · 2023: 49 · 2022: 46 · 2021: 72 · 2020: 43 · 2019: 51 · 2018: 61 · 2017: 55 · 2016: 47 · 2015: 66 · 2014: 82 · 2013: 74 · 2012: 112
FWCI
17.81
Type
journal article
Language
en
Open access
closed
Locations
Journal of the American Statistical Association (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
  • Physical Sciences › Mathematics › Statistics and Probability › Statistical Methods and Inference
Keywords
empirical Bayes, microarray data, gene expression, oligonucleotide microarrays, ionizing radiation, data reduction, simultaneous inference, false discovery rate, gene selection

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