The DOI matches its Crossref record; 90 of the 91 references it cites were found in a registry or an index.
Identification of Prolactin-Sensitive GABA and Kisspeptin Neurons in Regions of the Rat Hypothalamus Involved in the Control of Fertility
Abstract
High levels of circulating prolactin are known to cause infertility, but the precise mechanisms by which prolactin influences the neuroendocrine axis are yet to be determined. We used dual-label in situ hybridization to investigate whether prolactin-receptor (PRLR) mRNA is expressed in GnRH neurons. In addition, because γ-aminobutyric acidergic and kisspeptin neurons in the rostral hypothalamus are known to regulate GnRH neurons and, hence, might mediate the actions of prolactin, we investigated whether these neurons coexpress PRLR mRNA. (35)S-labeled RNA probes to detect PRLR mRNA were hybridized together with digoxigenin-labeled probes to detect either GnRH, Gad1/Gad2, or Kiss1 mRNA in the rostral hypothalamus of ovariectomized (OVX), estradiol-treated rats. Additional sets of serial sections were cut through the arcuate nucleus of OVX rats, without estradiol replacement, to examine coexpression of PRLR mRNA in the arcuate population of kisspeptin neurons. PRLR mRNA was highly expressed throughout the rostral preoptic area, particularly in periventricular regions surrounding the third ventricle, and there was a high degree of colocalization of PRLR mRNA in both Gad1/Gad2 and Kiss1 mRNA-containing cells (86 and 85.5%, respectively). In contrast, only a small number of GnRH neurons (<5%) was found to coexpress PRLR mRNA. In the arcuate nucleus of OVX rats, the majority of Kiss1 mRNA-containing cells also coexpressed PRLR mRNA. These data are consistent with the hypothesis that, in addition to a direct action on a small subpopulation of GnRH neurons, prolactin actions on GnRH neurons are predominantly mediated indirectly, through known afferent pathways.
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“only a few GnRH neurons express prolactin receptors (13-15), suggesting the existence of PRL-sensitive afferents.”
From Kisspeptin Overcomes GnRH Neuronal Suppression Secondary to Hyperprolactinemia in Humans (2022) · cites this paper as [14]
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“However, the localization of PRL receptors on kisspeptin neurons suggested that kisspeptin might mediate the inhibitory effects of hyperprolactinemia on GnRH secretion (13, 14).”
From Kisspeptin Overcomes GnRH Neuronal Suppression Secondary to Hyperprolactinemia in Humans (2022) · cites this paper as [14]
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Messager, S., Chatzidaki, E. E., Ma, D., Hendrick, A. G., Zahn, D., Dixon, J., Thresher, R. R., Malinge, I., Lomet, D., Carlton, M. B. L., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0409330102 · Cited by 1,242
A role for kisspeptins in the regulation of gonadotropin secretion in the mouse
Gottsch, M. L., Cunningham, M. J., Smith, J. T., Popa, S. M., Acohido, B. V., Crowley, W. F., Seminara, S., Clifton, D. K., & Steiner, R. A. (2004) · Endocrinology · doi:10.1210/en.2004-0431 · Cited by 1,161
Regulation of Kiss1 gene expression in the brain of the female mouse
Smith, J. T., Cunningham, M. J., Rissman, E. F., Clifton, D. K., & Steiner, R. A. (2005) · Endocrinology · doi:10.1210/en.2005-0488 · Cited by 1,102
Han, S.-K., Gottsch, M. L., Lee, K. J., Popa, S. M., Smith, J. T., Jakawich, S. K., Clifton, D. K., Steiner, R. A., & Herbison, A. E. (2005) · The Journal of Neuroscience · doi:10.1523/jneurosci.3328-05.2005 · Cited by 1,036
Irwig, M. S., Fraley, G. S., Smith, J. T., Acohido, B. V., Popa, S. M., Cunningham, M. J., Gottsch, M. L., Clifton, D. K., & Steiner, R. A. (2004) · Neuroendocrinology · doi:10.1159/000083140 · Cited by 930
Clarkson, J., & Herbison, A. E. (2006) · Endocrinology · doi:10.1210/en.2006-0787 · Cited by 875
Navarro, V. M., Gottsch, M. L., Chavkin, C., Okamura, H., Clifton, D. K., & Steiner, R. A. (2009) · The Journal of Neuroscience · doi:10.1523/jneurosci.1569-09.2009 · Cited by 779
Multimodal Influence of Estrogen upon Gonadotropin-Releasing Hormone Neurons
Herbison, A. E. (1998) · Endocrine Reviews · doi:10.1210/edrv.19.3.0332 · Cited by 618
Smith, J. T., Popa, S. M., Clifton, D. K., Hoffman, G. E., & Steiner, R. A. (2006) · Journal of Neuroscience · doi:10.1523/jneurosci.1618-06.2006 · Cited by 605
Wintermantel, T. M., Campbell, R. E., Porteous, R. W., Bock, D., Gröne, H., Todman, M. G., Korach, K. S., Greiner, E. F., Pérez, C. A., Schütz, G., et al. (2006) · Neuron · doi:10.1016/j.neuron.2006.07.023 · Cited by 598
Research works printed but not linked: 2
- Evans, 1982, Hypogonadism in hyperprolactinemia: proposed mechanisms, 77
- Petersen, 1993, GABAA receptor subunit mRNAs in cells of the preoptic area: colocalization with LHRH mRNA using dual label in situ hybridization histochemistry., Endocr J, 1, 29
Cited by
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Kisspeptins and Reproduction: Physiological Roles and Regulatory Mechanisms
Pinilla, L., Aguilar, E., Diéguez, C., Millar, R. P., & Tena‐Sempere, M. (2012) · Physiological Reviews · doi:10.1152/physrev.00037.2010 · Cited by 843
60 YEARS OF NEUROENDOCRINOLOGY: The hypothalamo-prolactin axis
Grattan, D. R. (2015) · Journal of Endocrinology · doi:10.1530/joe-15-0213 · Cited by 379
New insights in prolactin: pathological implications
Bernard, V., Young, J., Chanson, P., & Binart, N. (2015) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2015.36 · Cited by 244
Metabolic regulation of kisspeptin — the link between energy balance and reproduction
Navarro, V. M. (2020) · Nature Reviews Endocrinology · doi:10.1038/s41574-020-0363-7 · Cited by 240
Hyperprolactinemia-induced ovarian acyclicity is reversed by kisspeptin administration
Sonigo, C., Bouilly, J., Carré, N., Tolle, V., Caraty, A., Tello, J., Simony-Conesa, F.-J., Millar, R., Young, J., & Binart, N. (2012) · Journal of Clinical Investigation · doi:10.1172/jci63937 · Cited by 197
Metadata
- Authors
- Kokay, I. C., Petersen, S. L., & Grattan, D. R.
- Affiliations
- University of Otago (NZ)
- University of Massachusetts Amherst (US)
- Corresponding author
- Grattan, David R.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 2010-12-22
- Volume
- 152
- Issue
- 2
- Pages
- 526-535
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2133123261
- Cited by
- 131
- References
- 91
- Citations by year
- 2025: 5 · 2024: 14 · 2023: 7 · 2022: 4 · 2021: 6 · 2020: 12 · 2019: 10 · 2018: 13 · 2017: 8 · 2016: 11 · 2015: 10 · 2014: 10 · 2013: 5 · 2012: 9
- FWCI
- 7.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Neuroendocrine regulation and behavior
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Keywords
- prolactin, prolactin receptor, kisspeptin neurons, GABAergic neurons, GnRH neurons, hypothalamus, arcuate nucleus, preoptic area, fertility control, neuroendocrine regulation, infertility, estradiol, ovariectomy
- MeSH terms
- Animals, Female, Fertility, gamma-Aminobutyric Acid, Glutamate Decarboxylase, Hypothalamus, Neurons, Ovariectomy, Proteins, Receptors, Prolactin, Rats, Sprague-Dawley, In Situ Hybridization, Rats, Kisspeptins
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