Regional Sex Differences in Cell Nuclear Estrogen-Binding Capacity in the Rat Hypothalamus and Preoptic Area*

BROWN, THEODORE J.; HOCHBERG, RICHARD B.; ZIELINSKI, JAN E.; MACLUSKY, NEIL J.

doi:10.1210/endo-123-4-1761

Cited by 92Estrogen and related hormone effectsHypothalamic control of reproductive hormonesNeuroendocrine regulation and behavior

Regional Sex Differences in Cell Nuclear Estrogen-Binding Capacity in the Rat Hypothalamus and Preoptic Area*

BROWN, THEODORE J., HOCHBERG, RICHARD B., ZIELINSKI, JAN E., MACLUSKY, NEIL J.·1988·Endocrinology

DOI 10.1210/endo-123-4-1761

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Abstract

Estrogen binding was compared in cell nuclear KCl extracts from microdissected brain regions of gonadectomized-adrenalectomized male and female rats treated with a near-saturating dose of 17 beta-estradiol. Injection of 3.6 or 36.0 micrograms 17 beta-estradiol/kg BW, iv, 1 h before death resulted in a higher level of estrogen binding in the periventricular preoptic area (PVPOA), medial preoptic area, and ventromedial nucleus of the hypothalamus (VMN) of the female than in comparable tissue samples from the male. No significant sex differences in nuclear estrogen binding were observed in the arcuate-median eminence region, bed nucleus of the stria terminalis, or corticomedial amygdala. Scatchard analysis of saturation binding data revealed that the sex differences in cell nuclear estrogen binding in the PVPOA, medial preoptic area, and VMN reflect a difference in binding capacity rather than binding affinity. These in vitro biochemical findings were confirmed by autoradiographic studies. Gonadectomized-adrenalectomized animals were injected with 125I-labeled 11 beta-methoxy-16 alpha-iodoestradiol (2.0 micrograms/kg BW). Thin frozen sections (10 microns) through the preoptic area and hypothalamus were thaw-mounted onto microscope slides, then exposed against LKB Ultrofilm for 21 days. The autoradiographic images exhibited similar silver distributions and densities in males and females in the arcuate-median eminence region bed nucleus of the stria terminalis, and amygdala. Howev…

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No sex differences have been found in the number of estrogen receptors in the BST.
“Although no sex differences have been found in the number of estrogen receptors in the BST (Brown et al., 1988(Brown et al., , 1992) )”
From Sex differences in the effects of testosterone and its metabolites on vasopressin messenger RNA levels in the bed nucleus of the stria terminalis of rats (1994) · cites this paper as Brown et al., 1988

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Sex differences in estrogen receptor binding in the rat hypothalamus: effects of subsaturating pulses of estradiol
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Authors: BROWN, T. J., HOCHBERG, R. B., ZIELINSKI, J. E., & MACLUSKY, N. J.
Affiliations: Yale University (US)
ORCID: BROWN, THEODORE J.
MACLUSKY, NEIL J.
Venue: Endocrinology
Publisher: The Endocrine Society
Published: 1988-10
Volume: 123
Issue: 4
Pages: 1761-1770
ISSN: 0013-7227 (ISSN-L)
1945-7170
DOI: 10.1210/endo-123-4-1761
OpenAlex: W2003345618
Cited by: 92
References: 47
Citations by year: 2021: 1 · 2019: 1 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 2 · 2013: 2
FWCI: 3.77
Type: journal article
Language: en
Open access: closed
Locations: Endocrinology (publishedVersion)
PubMed (publishedVersion)
Funders: National Cancer Institute (CA-37799)
National Institute of Neurological Disorders and Stroke (NS-07807)
Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 13587)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
Keywords: sex differences, estrogen binding, nuclear estrogen receptor, hypothalamus, preoptic area, ventromedial hypothalamus, medial preoptic area, estradiol, gonadectomy, adrenalectomy, binding capacity, binding affinity, pituitary function, reproductive behavior
MeSH terms: Adrenalectomy, Animals, Cell Nucleus, Estradiol, Female, Hypothalamus, Male, Orchiectomy, Organ Specificity, Ovariectomy, Preoptic Area, Rats, Inbred Strains, Receptors, Estrogen, Reference Values, Sex Factors, Rats

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Regional Sex Differences in Cell Nuclear Estrogen-Binding Capacity in the Rat Hypothalamus and Preoptic Area*

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