The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Neurosteroids: Biosynthesis of Pregnenolone and Progesterone in Primary Cultures of Rat Glial Cells*
Abstract
Cells dissociated from newborn rat forebrains were established in long term primary cultures. The cultures were made up almost exclusively of oligodendrocytes and astrocytes, as confirmed by indirect immunofluorescence staining with monoclonal antibodies to galactocerebroside and glial fibrillary acidic protein, respectively. After 3 weeks of culture, the oligodendrocytes were also highly immunoreactive to monospecific polyclonal antibodies against cytochrome P450scc, an enzyme involved in the conversion of cholesterol to pregnenolone (P). Biosynthesis of [3H]cholesterol, [3H]P, and [3H]Pregn-5-ene-3 beta, 20 alpha-diol was demonstrated in these primary cultures by incubating cells with [3H]mevalonolactone in the presence of mevinolin and trilostane. The activity of the 2',3'-cyclic nucleotide 3'-phosphodiesterase enzyme, a documented indicator of oligodendrocyte differentiation, increased rapidly after day 10 of culture, together with the onset of steroid biosynthetic activity. Both reached a maximum at 3 weeks of culture and remained stable up to 6.5 weeks. In the absence of trilostane, [3H]P was converted by glial cell cultures to [3H]progesterone, [3H]5 alpha-pregnane-3,20-dione, and [3H]3 alpha-hydroxy-5 alpha-pregnan-20-one. The demonstration of P, pregn-5-ene-3 beta,20 alpha-diol, and progesterone synthesis by normal rat glial cells, once oligodendrocytes have undergone their differentiation process, brings additional support to the concept of "neurosteroids."
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Cites
Steroid hormone metabolites are barbiturate-like modulators of the GABA receptor
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Galactocerebroside is a specific cell-surface antigenic marker for oligodendrocytes in culture
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Pregnenolone and its sulfate ester in the rat brain
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“The transformation of P to progesterone was prevented by trilostane, but P was converted to unpolar compounds, which upon saponification, released P and, thus, were, likewise, P fatty acid esters, which have been previously characterized in the rat brain (5).”
BackgroundMethodNeurosteroids: Cytochrome P-450 scc in Rat Brain
Le Goascogne, C., Robel, P., Gouézou, M., Sananes, N., Baulieu, E.-E., & Waterman, M. (1987) · Science · doi:10.1126/science.3306919 · Cited by 363
Emergence of three myelin proteins in oligodendrocytes cultured without neurons.
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Cited by
Purdy, R. H., Morrow, A. L., Moore, P. H., & Paul, S. M. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.10.4553 · Cited by 951
Neurosteroids: biosynthesis and function of these novel neuromodulators
Compagnone, N. A., & Mellon, S. H. (2000) · Frontiers in Neuroendocrinology · doi:10.1006/frne.1999.0188 · Cited by 820
Multiple extracellular signals are required for long-term oligodendrocyte survival
Barres, B. A., Schmid, R., Sendnter, M., & Raff, M. C. (1993) · Development · doi:10.1242/dev.118.1.283 · Cited by 712
Lütjohann, D., Breuer, O., Ahlborg, G., Nennesmo, I., Sidén, A., Diczfalusy, U., & Björkhem, I. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.18.9799 · Cited by 682
Hojo, Y., Hattori, T.-A., Enami, T., Furukawa, A., Suzuki, K., Ishii, H.-T., Mukai, H., Morrison, J. H., Janssen, W. G. M., Kominami, S., et al. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2630225100 · Cited by 644
Metadata
- Authors
- TESTAS, I. J., HU, Z. Y., BAULIEUf, E. E., & ROBEL, A. P.
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1989-10
- Volume
- 125
- Issue
- 4
- Pages
- 2083-2091
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2008871773
- Cited by
- 366
- References
- 27
- FWCI
- 4.46
- Type
- journal article
- Language
- en
- Open access
- closed
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- TESTAS, I. J., HU, Z. Y., BAULIEUf, E. E., & ROBEL, A. P. (1989). Neurosteroids: Biosynthesis of Pregnenolone and Progesterone in Primary Cultures of Rat Glial Cells*. Endocrinology. https://doi.org/10.1210/endo-125-4-2083
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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