The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Insulin Stimulates Both Leptin Secretion and Production by Rat White Adipose Tissue
Abstract
Leptin, the peptide encoded by the obese gene, is secreted by adipose cells and plays a role in regulating food intake, energy expenditure, and adiposity. Because earlier studies suggested that insulin increases the expression of leptin, we investigated the effect of insulin on leptin secretion by adipose tissue. Epididymal fat pads were incubated in vitro in the presence or absence of insulin over a 4-h time course. Insulin increased leptin secretion by about 80% at all time points studied. After 10 min of insulin treatment, the amount of tissue-associated leptin was lower in insulin-stimulated tissue, presumably due to the increased secretion. At later times, both tissue-associated leptin and total leptin production were higher in insulin-treated tissue. In untreated, isolated adipose cells, immunostaining of leptin was detected in the endoplasmic reticulum by confocal microscopy. After insulin treatment, there were two populations of cells. In many cells, leptin staining became fainter and was restricted to a narrow band near the plasma membrane. However, in other cells the leptin-staining pattern was unchanged. Leptin did not colocalize with GLUT4, the glucose transporter isoform found primarily in insulin-responsive cells, in either basal or insulin-stimulated adipose cells. In this study, insulin increased both secretion and production of leptin by adipose tissue fragments. Interestingly, insulin appeared to stimulate the transport of leptin from the endoplasmic reticulum rather than acting on a pool of regulated secretory vesicles. (Endocrinology 138: 4463-4472, 1997)
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“therefore, some of the positive effects of insulin on the reproductive system might derive from its ability to stimulate leptin secretion.106”
From Pubertal development and regulation (2016) · cites this paper as [106]
In the citing paper
“Insulin is a major regulator of leptin production; therefore, some of the positive effects of insulin on the reproductive system might derive from its ability to stimulate leptin secretion.106”
From Pubertal development and regulation (2016) · cites this paper as [106]
Citations
Cites
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Research works printed but not linked: 6
- Barr, 1996, Insulin stimulates both the secretion and production of leptin by white fat tissue. Presented at the 36th Annual Meeting of the American Society for Cell Biology., Mol Biol Cell, 7, 448a
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- Parker, 1996, Leptin subcellular distribution and immunocytochemistry in adipocytes in basal, beta-adrenergic, and insulin stimulated states. Presented at the 56th Annual Meeting of the American Diabetes Association., Diabetes, 45, 153A
Cited by
Sorted by citations
Ahima, R. S., & Flier, J. S. (2000) · Annual Review of Physiology · doi:10.1146/annurev.physiol.62.1.413 · Cited by 1,428
Regulated transport of the glucose transporter GLUT4
Bryant, N. J., Govers, R., & James, D. E. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm782 · Cited by 1,183
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Pancreatic regulation of glucose homeostasis.
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Obesity and the polycystic ovary syndrome
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Metadata
- Authors
- Barr, V. A., Malide, D., Zarnowski, M. J., Taylor, S. I., & Cushman, S. W.
- Affiliations
- National Institutes of Health (US)
- Venue
- Endocrinology
- Publisher
- The Endocrine Society
- Published
- 1997-10-01
- Volume
- 138
- Issue
- 10
- Pages
- 4463-4472
- ISSN
- 0013-7227 (ISSN-L)
- 1945-7170
- OpenAlex
- W2126095470
- Cited by
- 297
- References
- 36
- Citations by year
- 2025: 2 · 2024: 8 · 2023: 6 · 2022: 9 · 2021: 5 · 2020: 8 · 2019: 9 · 2018: 9 · 2017: 10 · 2016: 9 · 2015: 11 · 2014: 9 · 2013: 4 · 2012: 11
- FWCI
- 5.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Keywords
- insulin, leptin, leptin secretion, white adipose tissue, epididymal adipose tissue, adipocytes, endoplasmic reticulum, GLUT4, obese gene, energy homeostasis
- MeSH terms
- Adipose Tissue, Animals, Endoplasmic Reticulum, Immunohistochemistry, Insulin, Male, Monosaccharide Transport Proteins, Muscle Proteins, Precipitin Tests, Proteins, Rats, Inbred Strains, Time Factors, Protein Biosynthesis, Microscopy, Confocal, Fluorescent Antibody Technique, Indirect, Leptin, Glucose Transporter Type 4, Rats
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