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Cited by 297Regulation of Appetite and ObesityAdipokines, Inflammation, and Metabolic DiseasesAdipose Tissue and Metabolism

The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.

Insulin Stimulates Both Leptin Secretion and Production by Rat White Adipose Tissue

Barr, Valarie A., Malide, Daniela, Zarnowski, Mary Jane, Taylor, Simeon I., Cushman, Samuel W.·1997·Endocrinology
DOI 10.1210/endo.138.10.5451
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Abstract

Leptin, the peptide encoded by the obese gene, is secreted by adipose cells and plays a role in regulating food intake, energy expenditure, and adiposity. Because earlier studies suggested that insulin increases the expression of leptin, we investigated the effect of insulin on leptin secretion by adipose tissue. Epididymal fat pads were incubated in vitro in the presence or absence of insulin over a 4-h time course. Insulin increased leptin secretion by about 80% at all time points studied. After 10 min of insulin treatment, the amount of tissue-associated leptin was lower in insulin-stimulated tissue, presumably due to the increased secretion. At later times, both tissue-associated leptin and total leptin production were higher in insulin-treated tissue. In untreated, isolated adipose cells, immunostaining of leptin was detected in the endoplasmic reticulum by confocal microscopy. After insulin treatment, there were two populations of cells. In many cells, leptin staining became fainter and was restricted to a narrow band near the plasma membrane. However, in other cells the leptin-staining pattern was unchanged. Leptin did not colocalize with GLUT4, the glucose transporter isoform found primarily in insulin-responsive cells, in either basal or insulin-stimulated adipose cells. In this study, insulin increased both secretion and production of leptin by adipose tissue fragments. Interestingly, insulin appeared to stimulate the transport of leptin from the endoplasmic reticulum rather than acting on a pool of regulated secretory vesicles. (Endocrinology 138: 4463-4472, 1997)

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Research works printed but not linked: 6

  1. Barr, 1996, Insulin stimulates both the secretion and production of leptin by white fat tissue. Presented at the 36th Annual Meeting of the American Society for Cell Biology., Mol Biol Cell, 7, 448a
  2. Houseknecht, 1996, Leptin secretion correlates with adipocyte size in genetic and dietary obesity. Presented at the 56th Annual Meeting of the American Diabetes Association., Diabetes, 45, 41A
  3. Weber, 1988, Methods for assessment of glucose transport activity and the number of glucose transporters in isolated rat adipose cells and membrane fractions. In: Kahn CR, Harrison LC (eds) The Insulin Receptor., Liss, New York, vol, 2, 171
  4. Malide, 1995, Insulin-stimulated GLUT 4 subcellular traffic in rat adipose cells: a confocal microscopy approach. Presented at the 35th Annual Meeting of the American Society for Cell Biology., Mol Biol Cell, 6, 58a
  5. Malide, 1996, Alteration of GLUT 4 subcellular trafficking in rat adipose cells by the PI3-kinase inhibitor wortmannin: a confocal microscopy approach. Presented at the 56th Annual Meeting of the American Diabetes Association., Diabetes, 45, 88A
  6. Parker, 1996, Leptin subcellular distribution and immunocytochemistry in adipocytes in basal, beta-adrenergic, and insulin stimulated states. Presented at the 56th Annual Meeting of the American Diabetes Association., Diabetes, 45, 153A

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Metadata

Authors
Barr, V. A., Malide, D., Zarnowski, M. J., Taylor, S. I., & Cushman, S. W.
Affiliations
National Institutes of Health (US)
ORCID
Venue
Endocrinology
Publisher
The Endocrine Society
Published
1997-10-01
Volume
138
Issue
10
Pages
4463-4472
ISSN
  • 0013-7227 (ISSN-L)
  • 1945-7170
DOI
10.1210/endo.138.10.5451
OpenAlex
W2126095470
Cited by
297
References
36
Citations by year
2025: 2 · 2024: 8 · 2023: 6 · 2022: 9 · 2021: 5 · 2020: 8 · 2019: 9 · 2018: 9 · 2017: 10 · 2016: 9 · 2015: 11 · 2014: 9 · 2013: 4 · 2012: 11
FWCI
5.77
Type
journal article
Language
en
Open access
closed
Locations
  • Endocrinology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
  • Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
Keywords
insulin, leptin, leptin secretion, white adipose tissue, epididymal adipose tissue, adipocytes, endoplasmic reticulum, GLUT4, obese gene, energy homeostasis
MeSH terms
Adipose Tissue, Animals, Endoplasmic Reticulum, Immunohistochemistry, Insulin, Male, Monosaccharide Transport Proteins, Muscle Proteins, Precipitin Tests, Proteins, Rats, Inbred Strains, Time Factors, Protein Biosynthesis, Microscopy, Confocal, Fluorescent Antibody Technique, Indirect, Leptin, Glucose Transporter Type 4, Rats

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