Increased VLDL-TG Fatty Acid Storage in Skeletal Muscle in Men With Type 2 Diabetes
Open accessAbstract
Context: Lipoprotein lipase (LPL) activity is considered the rate-limiting step of very-low-density-lipoprotein triglycerides (VLDL-TG) tissue storage, and has been suggested to relate to the development of obesity as well as insulin resistance and type 2 diabetes. Objective: The objective of the study was to assess the relationship between the quantitative storage of VLDL-TG fatty acids and LPL activity and other storage factors in muscle and adipose tissue. In addition, we examine whether such relations were influenced by type 2 diabetes. Design: We recruited 23 men (12 with type 2 diabetes, 11 nondiabetic) matched for age and body mass index. Postabsorptive VLDL-TG muscle and subcutaneous adipose tissue (abdominal and leg) quantitative storage was measured using tissue biopsies in combination with a primed-constant infusion of ex vivo triolein labeled [1-14C]VLDL-TG and a bolus infusion of ex vivo triolein labeled [9,10-3H]VLDL-TG. Biopsies were analyzed for LPL activity and cellular storage factors. Results: VLDL-TG storage rate was significantly greater in men with type 2 diabetes compared with nondiabetic men in muscle tissue (P = 0.02). We found no significant relationship between VLDL-TG storage rate and LPL activity or other storage factors in muscle or adipose tissue. However, LPL activity correlated with fractional VLDL-TG storage in abdominal fat (P = 0.04). Conclusions: Men with type 2 diabetes have increased VLDL-TG storage in muscle tissue, potentially contributing to increased intramyocellular triglyceride and ectopic lipid deposition. Neither muscle nor adipose tissue storage rates were related to LPL activity. This argues against LPL as a rate-limiting step in the postabsorptive quantitative storage of VLDL-TG.
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In response to 2 days of fasting, triacylglycerols accumulate in the liver of men but in the muscle of women.
“It has been reported that in response to 2 days of fasting, triacylglycerols accumulate in the liver of men, but in the muscle of women (77)”
From Sex differences in the metabolism of glucose and fatty acids by adipose tissue and skeletal muscle in humans (2025) · cites this paper as [77]
The Protocol describes an overnight fast, not two days of fasting. The Abstract and VLDL-TG storage rate section report muscle storage in men with and without type 2 diabetes; they do not report a fasting-induced liver-versus-muscle difference between men and women. Searches for two-day fasting and sex-specific hepatic and muscle triglyceride accumulation found no passage addressing the claim. (LLM, conf=0.78)
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- Authors
- Andersen, I. R., Søndergaard, E., Sørensen, L. P., Nellemann, B., Gormsen, L. C., Jensen, M. D., & Nielsen, S.
- Affiliations
- Departments of Endocrinology and Internal Medicine and
- Mayo Clinic (US)
- Aarhus University Hospital (DK)
- ORCID
- Venue
- The Journal of Clinical Endocrinology & Metabolism
- Publisher
- The Endocrine Society
- Published
- 2016-11-29
- Volume
- 102
- Issue
- 3
- Pages
- 831-839
- ISSN
- 0021-972X (ISSN-L)
- 1945-7197
- OpenAlex
- W2558169469
- Cited by
- 21
- References
- 45
- Citations by year
- 2025: 3 · 2024: 2 · 2022: 1 · 2021: 1 · 2020: 4 · 2019: 2 · 2018: 5 · 2017: 3
- FWCI
- 2.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Clinical Endocrinology & Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Aarhus Universitet
- Aarhus Universitetshospital
- Sundhed og Sygdom, Det Frie Forskningsråd
- Novo Nordisk
- Novo Nordisk Fonden (NNF09OC1012073, NNF13OC0007893)
- Medical Research Council
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
- Keywords
- VLDL triglycerides, lipoprotein lipase, skeletal muscle, type 2 diabetes, fatty acid storage, intramyocellular triglyceride, ectopic fat deposition, adipose tissue, insulin resistance, men
- MeSH terms
- Adult, Aged, Body Composition, Carbon Radioisotopes, Coenzyme A Ligases, Diabetes Mellitus, Type 2, Humans, Lipoprotein Lipase, Lipoproteins, VLDL, Male, Middle Aged, Tomography, X-Ray Computed, Triglycerides, Tritium, Absorptiometry, Photon, Case-Control Studies, Muscle, Skeletal, CD36 Antigens, Subcutaneous Fat, Diacylglycerol O-Acyltransferase
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