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Cited by 228

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Microsurgical and laser ablation analysis of interactions between the zones and layers of the tomato shoot apical meristem

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Reinhardt, Didier, Frenz, Martin, Mandel, Therese, Kuhlemeier, Cris·2003·Development
DOI 10.1242/dev.00596
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Abstract

Plants exhibit life-long organogenic and histogenic activity in a specialised organ, the shoot apical meristem. Leaves and flowers are formed within the ring-shaped peripheral zone, which surrounds the central zone, the site of the stem cells. We have undertaken a series of high-precision laser ablation and microsurgical tissue removal experiments to test the functions of different parts of the tomato meristem, and to reveal their interactions. Ablation of the central zone led to ectopic expression of the WUSCHELgene at the periphery, followed by the establishment of a new meristem centre. After the ablation of the central zone, organ formation continued without a lag. Thus, the central zone does not participate in organogenesis, except as the ultimate source of founder cells. Microsurgical removal of the external L1 layer induced periclinal cell divisions and terminal differentiation in the subtending layers. In addition, no organs were initiated in areas devoid of L1, demonstrating an important role of the L1 in organogenesis. L1 ablation had only local effects, an observation that is difficult to reconcile with phyllotaxis theories that invoke physical tension operating within the meristem as a whole. Finally, regeneration of L1 cells was never observed after ablation. This shows that while the zones of the meristem show a remarkable capacity to regenerate after interference, elimination of the L1layer is irreparable and causes terminal differentiation.

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Research works printed but not linked: 37

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Cited by

Metadata

Authors
Reinhardt, D., Frenz, M., Mandel, T., & Kuhlemeier, C.
Venue
Development
Publisher
The Company of Biologists
Published
2003-09-01
Volume
130
Issue
17
Pages
4073-4083
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
DOI
10.1242/dev.00596
OpenAlex
W1967250093
Cited by
228
References
3
FWCI
12.24
Type
journal article
Language
en
Open access
bronze

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