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Germline regulatory element of Oct-4 specific for the totipotent cycle of embryonal cells
Abstract
The totipotential stem cells of the pregastrulation mouse embryo which give rise to all embryonic somatic tissues and germ cells express Oct-4. The expression is downregulated during gastrulation and is thereafter only maintained in the germline lineage. Oct-4/lacZ transgenes were used to determine how this pattern of expression was achieved, and resulted in the identification of two separate regulatory elements. The distal element drives Oct-4 expression in preimplantation embryos, in migratory and postmigratory primordial germ cells but is inactive in cells of the epiblast. In cell lines this element is specifically active in embryonic stem and embryonic germ cells. The proximal element directs the epiblast-specific expression pattern, including downregulation during gastrulation; in cell lines its activity is restricted to epiblast-derived cells. Thus, Oct-4 expression in the germline is regulated separately from epiblast expression. This provides the first marker for the identification of totipotent cells in the embryo, and suggests that expression of Oct-4 in the totipotent cycle is dependent on a set of factors unique to the germline.
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Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe-Nebenius, D., Chambers, I., Schöler, H., & Smith, A. (1998) · Cell · doi:10.1016/s0092-8674(00)81769-9 · Cited by 3,392
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Rada-Iglesias, A., Bajpai, R., Swigut, T., Brugmann, S. A., Flynn, R. A., & Wysocka, J. (2011) · Nature · doi:10.1038/nature09692 · Cited by 2,233
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Kagey, M. H., Newman, J. J., Bilodeau, S., Zhan, Y., Orlando, D. A., van Berkum, N. L., Ebmeier, C. C., Goossens, J., Rahl, P. B., Levine, S. S., et al. (2010) · Nature · doi:10.1038/nature09380 · Cited by 1,939
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Bernstein, E., Kim, S. Y., Carmell, M. A., Murchison, E. P., Alcorn, H., Li, M. Z., Mills, A. A., Elledge, S. J., Anderson, K. V., & Hannon, G. J. (2003) · Nature Genetics · doi:10.1038/ng1253 · Cited by 1,627
Changing potency by spontaneous fusion
Ying, Q.-L., Nichols, J., Evans, E. P., & Smith, A. G. (2002) · Nature · doi:10.1038/nature729 · Cited by 1,494
Derivation of novel human ground state naive pluripotent stem cells
Gafni, O., Weinberger, L., Mansour, A. A., Manor, Y. S., Chomsky, E., Ben-Yosef, D., Kalma, Y., Viukov, S., Maza, I., Zviran, A., et al. (2013) · Nature · doi:10.1038/nature12745 · Cited by 1,046
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Hargreaves, D. C., & Crabtree, G. R. (2011) · Cell Research · doi:10.1038/cr.2011.32 · Cited by 923
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Saitou, M., Barton, S. C., & Surani, M. A. (2002) · Nature · doi:10.1038/nature00927 · Cited by 893
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Koh, K. P., Yabuuchi, A., Rao, S., Huang, Y., Cunniff, K., Nardone, J., Laiho, A., Tahiliani, M., Sommer, C. A., Mostoslavsky, G., et al. (2011) · Cell Stem Cell · doi:10.1016/j.stem.2011.01.008 · Cited by 776
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Pesce, M., & Schöler, H. R. (2001) · STEM CELLS · doi:10.1634/stemcells.19-4-271 · Cited by 771
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Kearns, N. A., Pham, H., Tabak, B., Genga, R. M., Silverstein, N. J., Garber, M., & Maehr, R. (2015) · Nature Methods · doi:10.1038/nmeth.3325 · Cited by 656
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Whyte, W. A., Bilodeau, S., Orlando, D. A., Hoke, H. A., Frampton, G. M., Foster, C. T., Cowley, S. M., & Young, R. A. (2012) · Nature · doi:10.1038/nature10805 · Cited by 602
Zhang, J., Tam, W.-L., Tong, G. Q., Wu, Q., Chan, H.-Y., Soh, B.-S., Lou, Y., Yang, J., Ma, Y., Chai, L., et al. (2006) · Nature Cell Biology · doi:10.1038/ncb1481 · Cited by 568
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Gidekel, S., Pizov, G., Bergman, Y., & Pikarsky, E. (2003) · Cancer Cell · doi:10.1016/s1535-6108(03)00270-8 · Cited by 441
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Bortvin, A., Eggan, K., Skaletsky, H., Akutsu, H., Berry, D. L., Yanagimachi, R., Page, D. C., & Jaenisch, R. (2003) · Development · doi:10.1242/dev.00366 · Cited by 434
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Anderson, R., Copeland, T. K., Schöler, H., Heasman, J., & Wylie, C. (2000) · Mechanisms of Development · doi:10.1016/s0925-4773(99)00271-3 · Cited by 313
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Shi, G., & Jin, Y. (2010) · Stem Cell Research & Therapy · doi:10.1186/scrt39 · Cited by 294
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NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers
Murakami, K., Günesdogan, U., Zylicz, J. J., Tang, W. W. C., Sengupta, R., Kobayashi, T., Kim, S., Butler, R., Dietmann, S., & Azim Surani, M. (2016) · Nature · doi:10.1038/nature16480 · Cited by 180
Knaupp, A. S., Buckberry, S., Pflueger, J., Lim, S. M., Ford, E., Larcombe, M. R., Rossello, F. J., de Mendoza, A., Alaei, S., Firas, J., et al. (2017) · Cell Stem Cell · doi:10.1016/j.stem.2017.11.007 · Cited by 127
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Radzisheuskaya, A., & Silva, J. C. R. (2014) · Trends in Cell Biology · doi:10.1016/j.tcb.2013.11.010 · Cited by 101
Akamatsu, W., DeVeale, B., Okano, H., Cooney, A. J., & van der Kooy, D. (2009) · The Journal of Neuroscience · doi:10.1523/jneurosci.4527-08.2009 · Cited by 72
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Kim, K.-P., Han, D. W., Kim, J., & Schöler, H. R. (2021) · Experimental & Molecular Medicine · doi:10.1038/s12276-021-00637-4 · Cited by 37
Self-citationPatterning and gastrulation defects caused by the tw18 lethal are due to loss of Ppp2r1a
Lange, L., Marks, M., Liu, J., Wittler, L., Bauer, H., Piehl, S., Bläß, G., Timmermann, B., & Herrmann, B. G. (2017) · Biology Open · doi:10.1242/bio.023200 · Cited by 19
Metadata
- Authors
- Yeom, Y. I., Fuhrmann, G., Ovitt, C. E., Brehm, A., Ohbo, K., Gross, M., Hübner, K., & Schöler, H. R.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 1996-03-01
- Volume
- 122
- Issue
- 3
- Pages
- 881-894
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W1957763880
- Cited by
- 864
- References
- 0
- FWCI
- 5.07
- Type
- journal article
- Language
- en
- Open access
- closed
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