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Cited by 624

The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.

Mutations affecting the formation and function of the cardiovascular system in the zebrafish embryo

Open access
Stainier, Didier Y. R., Fouquet, Bernadette, Chen, Jau-Nian, Warren, Kerri S., Weinstein, Brant M., Meiler, Steffen E., Mohideen, Manzoor-Ali P. K., Neuhauss, Stephan C. F., Solnica-Krezel, Liliana, Schier, Alexander F., Zwartkruis, Fried, Stemple, Derek L., Malicki, Jarema, Driever, Wolfgang, Fishman, Mark C.·1996·Development
DOI 10.1242/dev.123.1.285
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Abstract

As part of a large-scale mutagenesis screen of the zebrafish genome, we have identified 58 mutations that affect the formation and function of the cardiovascular system. The cardiovascular system is particularly amenable for screening in the transparent zebrafish embryo because the heart and blood vessels are prominent and their function easily examined. We have classified the mutations affecting the heart into those that affect primarily either morphogenesis or function. Nine mutations clearly disrupt the formation of the heart. cloche deletes the endocardium. In cloche mutants, the myocardial layer forms in the absence of the endocardium but is dysmorphic and exhibits a weak contractility. Two loci, miles apart and bonnie and clyde, play a critical role in the fusion of the bilateral tubular primordia. Three mutations lead to an abnormally large heart and one to the formation of a diminutive, dysmorphic heart. We have found no mutation that deletes the myocardial cells altogether, but one, pandora, appears to eliminate the ventricle selectively. Seven mutations interfere with vascular integrity, as indicated by hemorrhage at particular sites. In terms of cardiac function, one large group exhibits a weak beat. In this group, five loci affect both chambers and seven a specific chamber (the atrium or ventricle). For example, the weak atrium mutation exhibits an atrium that becomes silent but has a normally beating ventricle. Seven mutations affect the rhythm of the heart causing, for example, a slow rate, a fibrillating pattern or an apparent block to conduction. In several other mutants, regurgitation of blood flow from ventricle to atrium is the most prominent abnormality, due either to the absence of valves or to poor coordination between the chambers with regard to the timing of contraction. The mutations identified in this screen point to discrete and critical steps in the formation and function of the heart and vasculature.

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Metadata

Authors
Stainier, D. Y. R., Fouquet, B., Chen, J.-N., Warren, K. S., Weinstein, B. M., Meiler, S. E., Mohideen, M.-A. P. K., Neuhauss, S. C. F., Solnica-Krezel, L., Schier, A. F., Zwartkruis, F., Stemple, D. L., Malicki, J., Driever, W., & Fishman, M. C.
Venue
Development
Publisher
The Company of Biologists
Published
1996-12-01
Volume
123
Issue
1
Pages
285-292
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
DOI
10.1242/dev.123.1.285
OpenAlex
W2141279088
Cited by
624
References
26
FWCI
7.70
Type
journal article
Language
en
Open access
green

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